A highly sensitive and quantitative time resolved fluorescent microspheres lateral flow immunoassay for streptomycin and dihydrostreptomycin in milk, honey, muscle, liver, and kidney

Jiang, Jiali; Luo, Pengjie; Liang, Jinxuan; Shen, Xing; Lei, Hongtao; Li, Xiangmei

doi:10.1016/j.aca.2021.339360

Cited by 36 publications

(13 citation statements)

References 31 publications

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“…Although signal labeling can improve the sensitivity of LFA, unfortunately, the detection method is still limited to the detection of AGs residues in partial foods of animal origin. In order to expand the application scope of this method, Jiang et al established a time-resolved fluorescence microsphere lateral-flow immunoassay (TRFM-LFIA) with a portable fluorometer for rapid detection of STR and DHS in the foods of animal origin [ 158 ]. With this method, the sample pretreatment is very simple and can be completed quickly in 8 min.…”

Section: Affinity-based Analysis Methods For Aminoglycoside Residuesmentioning

confidence: 99%

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Liu

Chen

et al. 2023

Foods

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With the increasingly serious problem of aminoglycoside antibiotic residues, it is imperative to develop rapid, sensitive and efficient detection methods. This article reviews the detection methods of aminoglycoside antibiotics in animal-derived foods, including enzyme-linked immunosorbent assay, fluorescent immunoassay, chemical immunoassay, affinity sensing assay, lateral flow immunochromatography and molecular imprinted immunoassay. After evaluating the performance of these methods, the advantages and disadvantages were analyzed and compared. Furthermore, development prospects and research trends were proposed and summarized. This review can serve as a basis for further research and provide helpful references and new insights for the analysis of aminoglycoside residues. Accordingly, the in-depth investigation and analysis will certainly make great contributions to food safety, public hygiene and human health.

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Section: Affinity-based Analysis Methods For Aminoglycoside Residuesmentioning

confidence: 99%

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Liu

Chen

et al. 2023

Foods

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“…Consequently, when the sample is containing sufficient ENR, it will fully bind with the detection reagent which in turn blocks the reaction of the detection reagent with the capture reagent by capturing the detection reagent completely. This leads to the invisibility of the T line on the NC membrane which indicates positive results ( 46 ). To confirm the validity of the testing procedure of the test strip, the control line should be visible always.…”

Section: Methodsmentioning

confidence: 99%

A highly sensitive lateral flow immunoassay for the rapid and on-site detection of enrofloxacin in milk

et al. 2022

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Enrofloxacin (ENR) is a veterinary antibiotic used to treat bacterial infections in livestock. It chiefly persists in foods and dairy products, which in turn pose severe risks to human health. Hence it is very important to detect the ENR in foods and dairy products to safeguard human health. Herein, we attempted to develop a single-step detection lateral flow immunochromatographic assay (LFIA) using gold nanoparticles (AuNPs) for the rapid and on-site detection of ENR in milk samples. An anti-enrofloxacin monoclonal antibody (ENR-Ab) was conjugated with AuNPs for the specific detection of ENR in milk samples. For sensitivity improvement, many optimization steps were conducted on LFIA test strips. The visual limit of detection (vLOD) was found to be 20 ng/ml with a cut-off value of 50 ng/ml in the milk samples. The obtained LOD and cut-off value were within the safety limit guidelines of the Ministry of food and drug safety, South Korea. The test strip showed negligible cross-reactivity with ENR analogs, and other components of antibiotics, this indicates the high specificity of the LFIA test strip towards ENR. The designed test strip showed good reliability. The visual test results can be seen within 10 min without the need for special equipment. Therefore, the test strip can be employed as a potential detection strategy for the qualitative on-site detection of enrofloxacin in milk samples.

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“…Streptomycin (STR) and its derivative dihydrostreptomycin (DSM) are aminoglycoside antibiotics (AGs) antibiotics show broad‐spectrum antimicrobial activities and are used to prevent and treat diseases of animal diseases. It is also used by beekeepers to treat rot diseases and the European Foulbrood (EFB) of bees [6,7] . Derivatives of streptomycin are also used to treat fire blight disease seen in apple trees.…”

Section: Introductionmentioning

confidence: 99%

Detection of Multiple Antibiotic Residues in Turkish Pine and Blossom Honeys Using LC–MS/MS Method

Gün,

Dursun,

Arıcı

et al. 2024

Chemistry & Biodiversity

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Due to the high demand for honey, beekeepers often feed the bees with antibiotics to protect honeybees against illnesses; determination of veterinary drugs and their residues in bee products especially in honey is gaining importance. In this study, commercially available 15 different brands, a total of 22 honey (14 blossoms and 8 pines) samples obtained from 5 chain supermarkets in city of Bingöl and Diyarbakır, Turkey were analysed for 29 antibiotic residues. These antibiotics belonging to 10 different categories, including tetracyclines, aminoglycosides, macrolides, sulfonamides, fluoroquinolones, benzimidazoles, anthelmintic, amphenicols, quinolines, and oxazolidines. For the qualitative and quantitative determination of the antibiotics, a triple quadrupole liquid chromatography tandem mass spectrometry (LC‐MS/MS) was used. A total of 10 out of 22 honey (8 blossom, 57.14% and 2 pine, 25%) samples were found to be positive for antibiotics. Among the tested antibiotics, tetracycline, dihydrostreptomycin, streptomycin, erythromycin, and sulfadimidine were detected in the honey samples. Dihydrostreptomycin and sulfadimidine were detected in 6 samples, erythromycin was determined in 4 samples, streptomycin was found in 2 samples, and lastly tetracycline was detected only in one sample. Good linearities were also achieved ranging between R2 = 0.987 and 0.999.

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A highly sensitive and quantitative time resolved fluorescent microspheres lateral flow immunoassay for streptomycin and dihydrostreptomycin in milk, honey, muscle, liver, and kidney

Cited by 36 publications

References 31 publications

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

Affinity-Based Analysis Methods for the Detection of Aminoglycoside Antibiotic Residues in Animal-Derived Foods: A Review

A highly sensitive lateral flow immunoassay for the rapid and on-site detection of enrofloxacin in milk

Detection of Multiple Antibiotic Residues in Turkish Pine and Blossom Honeys Using LC–MS/MS Method

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