A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection

Wetzel, Thierry; Candresse, Thierry; Macquaire, G.; Ravelonandro, Michel; Dunez, Jean

doi:10.1016/0166-0934(92)90122-t

Cited by 259 publications

(127 citation statements)

References 10 publications

Supporting

Mentioning

115

Contrasting

Unclassified

Order By: Relevance

“…The presence of PPV in leaf extracts from interspecific hybrids and selections was verified by immunocapturereverse transcription-polymerase chain reaction (IC-RT-PCR) in the last two years of evaluation, in 2007 and 2008. The IC-RT-PCR protocol of Wetzel et al (1992) was used. The Robust II RT-PCR kit (Finnzymes, Espoo, Finland ) and the pair of oligonucleotide primers P1/P2 (Candresse et al 1995) were applied in IC-RT-PCR detection.…”

Section: Methodsmentioning

confidence: 99%

Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection

Polák¹,

Oukropec²

2010

Plant Prot. Sci.

View full text Add to dashboard Cite

Polák J., Oukropec I. (2010): Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection. Plant Protect. Sci., 46: 139-144.Interspecific hybrids of Prunus persica, Barier, Fire, Cadaman, GF-677, and Prunus sp. hybrids and selections, MRS, NBS 540-73, and Pumiselect were evaluated for resistance to Plum pox virus. Hybrids were grafted onto trees of a peach cultivar artificially infected with PPV and evaluated for six years for resistance to the virus. The relative concentration of PPV protein was determined by semiquantitative ELISA in June every year. The presence of PPV in peach hybrids was confirmed by IC- RT-PCR in 2007-2008. The presence and intensity of PPV symptoms were evaluated monthly from May to September. The hybrid GF-677 (P. amygdalus × P. persica) was confirmed as highly resistant to PPV. Hybrids Cadaman (P. davidiana × P. persica) and Fire (P. amygdalus × P. persica) were characterized as resistant to PPV. Hybrids GF-677, Cadaman and Fire were selected as candidate sources of resistance to be crossed with peach cultivars susceptible to PPV.

show abstract

Section: Methodsmentioning

confidence: 99%

Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection

Polák¹,

Oukropec²

2010

Plant Prot. Sci.

View full text Add to dashboard Cite

show abstract

“…The application of Polymerase Chain Reaction (PCR) for Plum pox virus improved the detection and characterization of this pathogen (Wetzel et al, 1991b;Wetzel et al, 1992). The most frequently targeted region in PPV analysis is the highly variable 5'-terminal part of the CP gene.…”

Section: Plum Pox Virus Strain Variabilitymentioning

confidence: 99%

Plum pox virus strains: Diversity and geographical distribution in Serbia

Jevremović

Paunović

2014

Pesticidi i fitomedicina

View full text Add to dashboard Cite

Plum pox virus (PPV) is the causal agent of Sharka disease. Since its discovery, Sharka has been considered as a calamity in plum orchards. PPV is present worldwide in many Prunus species, causing great economic losses. In highly susceptible plum varieties, such as Pozegaca, PPV causes a premature fruit drop and reduces fruit quality, which leads to total yield loss. Eight PPV strains (PPV-M, PPV-D, PPV-EA, PPV-C, PPV-Rec, PPV-W, PPV-T and PPVCR) have been recognized so far. Three major strains (PPV-M, PPV-D and PPV-Rec) are the most widely dispersed and occur frequently in many European countries. Other strains are of minor importance due to their limited host preferences or geographic distribution. So far, all three major strains have been identified in Serbia. In this paper, we provide a comprehensive overview of the research into Plum pox virus variability in Serbia. [Projekat Ministarstva nauke Republike Srbije, br TR-20013A i br. TR-31064. This study was partially supported by the ECONET grant no. 10159PL, the FP7 project SharCo]

show abstract

“…The standard sample extraction procedure for RT-PCR detection of ASGV is based on nucleic acid isolation (total RNA) (7,8,11), which is time-consuming and not amenable for processing large numbers of samples. Potentially improved sample processing procedures for plant virus detection by PCR have been reported and include immunocapture (24), direct binding (19), print capture (17), or direct application of clarified plant extracts in reaction tubes (25). This paper reports the design of specific primers based on the comparison of partial nucleotide sequences established for four European ASGV isolates and that of the Japanese isolate published by Yoshikawa et al (26).…”

mentioning

confidence: 99%

Detection of Apple Stem Grooving Virus in Dormant Apple Trees with Crude Extracts as Templates for One-Step RT-PCR

et al. 1998

View full text Add to dashboard Cite

Partial nucleotide sequences of amplification products obtained from four European apple stem grooving virus (ASGV) isolates using degenerate primers showed 80 to 85% similarity with the published ASGV sequence of a Japanese strain but 98 to 100% identities among themselves. Based on these sequences, two ASGV-specific primers (ASGV4F-ASGV4R) were designed to amplify a 574-bp fragment located in the putative viral RNA polymerase. With these primers, six European and five American ASGV isolates, maintained in herbaceous hosts (Chenopodium quinoa, Nicotiana glutinosa, and N. occidentalis) or in apple trees, were readily detected by reverse transcription-polymerase chain reaction (RT-PCR). Using these specific ASGV primers, dsRNA preparations have been shown to constitute good templates for reliable amplification of ASGV sequences from leaves and bark tissues of apple trees, both in a two-step RT-PCR protocol and in the one-step Titan One-Tube RT-PCR. System. Furthermore, the one-step RT-PCR system allowed a specific amplification of ASGV sequences directly from clarified crude extracts of leaves and bark tissues of apple trees during both active growth and the dormant season.

show abstract

A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection

Cited by 259 publications

References 10 publications

Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection

Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection

Plum pox virus strains: Diversity and geographical distribution in Serbia

Detection of Apple Stem Grooving Virus in Dormant Apple Trees with Crude Extracts as Templates for One-Step RT-PCR

Contact Info

Product

Resources

About