A Highly Sensitive, Multiplex Broad-Spectrum PCR-DNA-Enzyme Immunoassay and Reverse Hybridization Assay for Rapid Detection and Identification of Chlamydia trachomatis Serovars

Quint, Koen D.; Doorn, Leen Jan Van; Kleter, Bernhard; Koning, Maurits N.C. de; Munckhof, Henk A. M. van den; Morré, Servaas A.; Harmsel, Bram ter; Weiderpass, Elisabete; Harbers, Gonneke; Melchers, Willem J. G.; Quint, Wim

doi:10.2353/jmoldx.2007.070011

Cited by 43 publications

(47 citation statements)

References 28 publications

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“…Importantly, these mixed preparations were arbitrarily prepared with a 100-fold difference in bacterial load, and it should be emphasized that a natural mixed infection could comprise a severalfold-higher difference between coinfecting serovars, which could ultimately affect the detection of a serovar at lower levels. The frequency of multiple serovars identified in this study (2.4%) is comparable to those in other C. trachomatis typing studies of between 1.5% and 5.4% (8,15,19,20,33). Interestingly, some studies have described multiple infections with frequencies as high as 8.7% and 13% (14,31) which principally involved use of PCR followed by reverse line blot procedures.…”

Section: Discussionsupporting

confidence: 68%

“…C. trachomatis serovars can be subdivided into three distinct phylogenetic clades based on the ompA gene: the B group (comprising B/Ba, D, E, L1, and L2), the C group (comprising A, C, H, I, J, K, and L3), and the intermediate (I) group (comprising F and G). Although there is no correlation of phylogenetic clades with characteristic disease, these clades/groups have value for the development of more rapid screening assays for predicting serovars (19,20,33).…”

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confidence: 99%

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Development and Evaluation of anompAQuantitative Real-Time PCR Assay forChlamydia trachomatisSerovar Determination

et al. 2010

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Section: Discussionsupporting

confidence: 68%

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confidence: 99%

Development and Evaluation of anompAQuantitative Real-Time PCR Assay forChlamydia trachomatisSerovar Determination

et al. 2010

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“…Rijswijk, The Netherlands) and as described previously (12,14). Briefly, amplification was 130 performed with the Ct-DT-PCR, followed by Ct detection with the Ct-DT-DEIA.…”

Section: Ct-dt Amplification Detection and Genotyping 127mentioning

confidence: 99%

“…The Ct-Detection genoTyping (DT) assay 79 consists of a Ct amplification step (PCR), a Ct Detection step (DNA Enzyme Immuno Assay; 80 DEIA) and a Ct genotyping step (Reverse Hybridization Assay; RHA). This assay is an 81 alternative for Omp1 sequencing by differentiating between the 14 major serovars (12,13). 82…”

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confidence: 99%

“…Because LGV Ct 75 infections require longer antibiotic treatment, it is highly recommended to differentiate them 76 from non-LGV serovars (9). 77 A number of reverse line blot assays were developed making genotyping faster and less 78 laborious, compared to sequencing (10)(11)(12). The Ct-Detection genoTyping (DT) assay 79 consists of a Ct amplification step (PCR), a Ct Detection step (DNA Enzyme Immuno Assay; 80 DEIA) and a Ct genotyping step (Reverse Hybridization Assay; RHA).…”

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Comparison of three genotyping methods to identify Chlamydia trachomatis genotypes in positive men and women

Quint

Bom

Bruisten

et al. 2010

Molecular and Cellular Probes

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General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

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Prevalence, incidence and clearance of human papillomavirus infection among young primiparous pregnant women in Kampala, Uganda

Banura

Franceschi

Doorn

et al. 2008

Intl Journal of Cancer

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The proportion of women who have already been exposed to human papillomavirus (HPV) infection by the time they first become pregnant, and the influence of pregnancy and delivery on the course of HPV infection are unclear. In Kampala, Uganda, 987 young primiparous pregnant women aged <25 years had gynaecological examination and liquid-based cytology. In the follow-up, women acted as their own controls, i.e., 1st/2nd versus 3rd trimesters (105 women), and during pregnancy versus after delivery (289 women). HPV was assessed using highly sensitive PCR assays. Prevalence of HPV and HIV infections at baseline were 60.0% and 7.3%, respectively. HPV16 and 18 were detected in 8.4% and 5.8%, respectively, i.e., less frequently than HPV51 (8.7%) and 52 (12.1%). At follow-up new HPV infections were detected in 42.9% of women between the 1st/2nd and 3rd trimesters, and 38.1% between pregnancy and delivery, but 50.4% and 71.8% of HPV infections, respectively, cleared, leaving HPV prevalence unchanged in the different periods. Prevalence of cytological abnormalities diminished after delivery (from 21.2% to 12.4%). Presence of genital warts and sexually transmitted infections other than HPV were the strongest risk factors for prevalent or incident HPV infection. Clearance was lower among HIV-positive women. In conclusion, HPV prevalence was high in primiparous women in Uganda, but pregnancy did not seem to be a period of special vulnerability to the infection. ' 2008 Wiley-Liss, Inc.Key words: human papillomavirus; HIV; Uganda; pregnant adolescents; epidemiology Prophylactic vaccines against human papillomavirus (HPV) 16 and 18 are recommended for girls who are not yet sexually active.1 In low-resource countries where the burden of cervical cancer is high, however, a catching up of less young women when they have contacts with medical facilities (e.g., at first delivery) may be worth considering if the number of young women not yet infected by HPV vaccine types is low. Furthermore, although high parity and young age at first birth are risk factors for cervical cancer, even after adjustment for sexual behavior, 2 relatively few studies have provided information on the influence of pregnancy status on the incidence and clearance of HPV infection. Depending upon the study, HPV detection in pregnant women was shown to be higher than, 3,4 or similar to 5-8 HPV infection among nonpregnant women.To further elucidate this issue, we evaluated prevalence, incidence and clearance of HPV infection between the 1st/2nd and 3rd trimesters of pregnancy, and between pregnancy and after delivery, among young primiparous pregnant women in Kampala, Uganda. Material and methods Study population and follow-upPrimiparous pregnant women aged below 25 years residing within a 20 km radius for the previous 6 months and presenting themselves for antenatal services at the Naguru Health Centre, located in a suburb of Kampala, Uganda, between May and November 2004, were invited to join the study. Refusals to participate were few (approximately 2%).Trained m...

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A Highly Sensitive, Multiplex Broad-Spectrum PCR-DNA-Enzyme Immunoassay and Reverse Hybridization Assay for Rapid Detection and Identification of Chlamydia trachomatis Serovars

Cited by 43 publications

References 28 publications

Development and Evaluation of anompAQuantitative Real-Time PCR Assay forChlamydia trachomatisSerovar Determination

Development and Evaluation of anompAQuantitative Real-Time PCR Assay forChlamydia trachomatisSerovar Determination

Comparison of three genotyping methods to identify Chlamydia trachomatis genotypes in positive men and women

Prevalence, incidence and clearance of human papillomavirus infection among young primiparous pregnant women in Kampala, Uganda

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