A Highly Sensitive Nanostructure-Based Surface Covalently Modification of Gold for Electrochemical Sensing of Epinephrine in Presence of Uric Acid and Acetaminophen

Abstract

In the present paper, the use of a gold electrode modified by 2-hydroxy-N′1-[(E)-1-(3-methyl-2-thienyl) methylidene] benzohydrazide self assembled monolayer (TMBH) for the determination of Epinephrine (Epi) in the presence of Uric acid (UA) and Acetaminophen (ACOP) was described. Initially, the formation of the TMBH at the surface of gold electrode investigated by electrochemical methods. At the optimum pH of 6.0, the oxidation of Epi occurs at a potential about 28 mV less positive than that of an unmodified gold electrode. The values of electron transfer coefficients (α = 0.55), catalytic rate constant (k = 1.2 × 10−2 M−1 s−1) and diffusion coefficient (D = 3.6 × 10−6 cm2 s−1) were calculated for Epi, using electrochemical approaches. Based on hydrodynamic differential pulse amprometry, the oxidation of Epi exhibited a dynamic range between 3.0 and 100.0 μM and a detection limit (3σ) of 0.08 μM. Furthermore, simultaneous determination of Epi and UA or ACOP at the modified electrode was described. The Au-TMBH SAM-modified electrode was shown agreeable recoveries in two types of serum; healthy and disease human blood serum.

Immobilization of a novel synthesized Schiff base on the surface of a gold electrode for the electrochemical study of adrenaline

“…The strong p-p interactions between AD and SITP, unlike the weak interactions between AA or UA and this molecule, may accelerate the electron transfer. 32…”

Graphene-Palladium Composite for the Simultaneous Electrochemical Determination of Epinephrine, Ascorbic acid and Uric Acid

“…Studies on the electrochemistry of EP is important because it regulates heart beat and plays a significant role in treatment of diseases such as cardiac arrest, anaphylaxis, hypertension, glaucoma, bronchitis, bronchial asthma and other allergic conditions. 1 Uric acid (UA) is the primary product of purine metabolism 2 and its level ranges from 41-88 mgmL −1 in serum. 3 Abnormal concentration level of UA is an indication of several diseases, such as gout, hyperuricemia and Lesch -Nyhan syndrome, Leukemia, Pneumonia etc.…”

“…Among the different nanomaterials graphene based materials received great interest because of their fascinating surface structure and versatile electrical and mechanical properties. [25][26][27] Graphene is one atom thick planar sheets of sp 2 bonded carbon atoms that are densely packed in a honeycomb crystal lattice, which is primarily obtained from reduction of graphene oxide. 28 Functionalization of graphene leads to improvement of surface area and related properties resulting in enhanced electrode kinetics, when used for electrode modification.…”