A highly sensitive naphthalimide‐based fluorescent probe for detection of Cu²⁺ via selective hydrolysis reaction and its application in practical samples

Abstract

A novel fluorescent probe CN3, containing 1,8‐naphthalimide and picolinate units, was synthesized, and its structure was characterized by 1H nuclear magnetic resonance spectroscopy (H NMR), 13C nuclear magnetic resonance spectroscopy (C NMR), and mass spectroscopy techniques. The detection property of CN3 toward copper ions (Cu2+) has been investigated in ethanol–HEPES buffer (v/v = 1/1, pH = 7.40) solution by UV–Vis absorption and fluorescence emission spectra. The results showed that CN3 had a highly selective and sensitive fluorescence quenching response to Cu2+, which was attributed to the generation of weak fluorescent N‐ethyl‐4‐hydroxyphenyl‐1,8‐ naphthalimide (compound 2) in polar ethanol–HEPES buffer (v/v = 1/1, pH = 7.40) via selective hydrolysis reaction. The detection of CN3 for Cu2+ was not influenced in the presence of other competing metal ions, and the limit of detection was as low as 50.0 nM. Therefore, the color of CN3 changed from colorless to yellowish when the Cu2+ was added. Furthermore, the fluorescent probe CN3 was utilized to detect Cu2+ in real water samples with fine performance.

Research Progress on Naphthalimide Fluorescent Probes

“…The new fluorescent probe 5 , which contains 1,8‐naphthalimide and picolinate moiety, was prepared for detecting Cu 2+ by Zhu's team (Figure 2). [ 17 ] Probe 5 shows a fluorescence quenching response to Cu 2+ with a LOD (52 nM). Furthermore, upon binding with Cu 2+ , an obvious color change in the sample solution from colorless to pale yellow can be observed.…”

A mixed-valence [CoII 4CoIII 2] cluster with defect disk-shaped topology

“…Polynuclear coordination compounds of 3d transition metals have attracted continued attention for several decades due to their structural novelty, interesting catalytic (Dastidar & Chattopadhyay, 2022;Shul'pin & Shul'pina, 2021;Nesterov & Nesterova, 2018;Jing et al, 2020) and biological properties (Hazari et al, 2017;Amtul et al, 2002;Azizian et al, 2012;Tanaka et al, 2003), and their potential as single-molecule magnets (SMMs) (Radu et al, 2017;Pattacini et al, 2011). Among numerous polynuclear 3d complexes, cobalt clusters have received particular interest because of their pleasing topological aesthetics (Brechin et al, 1997;Cao et al, 2013), their relevance to dioxygen reduction (Monte-Pe ´rez et al, 2017) and their fascinating magnetic properties (Liu et al, 2020;Sarto et al, 2018;Li et al, 2020;Ma et al, 2012).…”

Quantitative Assessment of Copper(II) in Wilson’s Disease Based on Photoacoustic Imaging and Ratiometric Surface-Enhanced Raman Scattering

“…Copper is an essential trace metal element for the human body. It plays a variety of important roles in the body, such as catalyzing, maintaining the health of the nervous system, and helping to maintain the body’s hematopoietic function. − A physiological imbalance of copper can lead to many serious diseases, such as kidney damage, liver damage, Alzheimer’s disease, and Wilson’s disease (WD). ,− WD is an autosomal recessive hereditary disease, which is a congenital disorder of copper metabolism. Specifically, mutations in the gene encoding the copper transporter ATP7B protein in organisms prevent the synthesis of copper cyanine, leading to excessive accumulation of Cu 2+ in the liver, kidney, and brain, , which causes liver damage, mental disorders, and corneal pigmentation in patients.…”

“…At present, the common detection methods for Cu 2+ include colorimetry, ICP-MS, fluorescence imaging, and so on. ,,,− However, the former two methods are expensive and require cumbersome testing procedures, making them unsuitable for in vivo detection. − For fluorescence imaging, its penetration depth (about 1 mm) in living subjects is too small for in vivo detection due to the common optical attenuation phenomenon caused by the influence of solvents, reaction conditions, and other factors . Photoacoustic (PA) imaging is an emerging noninvasive imaging modality with a large penetration depth (generally around 10 cm), − making it suitable for in vivo imaging of the structure of biological tissues, − biomolecules, , enzymes, etc . , For example, Cha et al .…”