A highly specific competitive direct enzyme immunoassay for sterigmatocystin as a tool for rapid immunochemotaxonomic differentiation of mycotoxigenic<i>Aspergillus</i>species

Wegner, S.; Bauer, Julia; Dietrich, Richard; Märtlbauer, Erwin; Usleber, Ewald; Gottschalk, Christoph; Groß, Matthias

doi:10.1111/lam.12702

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…For mycotoxin analysis of PBMA samples, EIA were performed using microtiter plates (MaxiSorp, Nunc, Roskilde, Denmark) as described earlier for AFB 1 (Gathumbi et al 2001 ), STC (Wegner et al 2016 ), OTA (Schneider et al 2001 ), DON (Curtui et al 2003 ), and T-2/HT-2 (Esgin et al 1989 ). All EIA were performed based on competitive direct test format, using the double antibody method for DON and T-2/HT-2.…”

Section: Methodsmentioning

confidence: 99%

Enzyme immunoassays for the detection of mycotoxins in plant-based milk alternatives: pitfalls and limitations

et al. 2022

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Plant-based milk alternatives (PBMAs) are a potential source of mycotoxin uptake. To ensure food safety, simple and rapid testing methods of PBMAs for mycotoxins are therefore required. This study investigated the applicability of enzyme immunoassay (EIA) methods for direct testing of PBMAs without sample extraction. Mycotoxin analyses included aflatoxin B1 (AFB1), sterigmatocystin (STC), ochratoxin A (OTA), deoxynivalenol (DON), and T-2/HT-2-toxin (T-2/HT-2). It was found that the PBMA matrix negatively affected the EIA to varying degrees, thus affecting the reliability of the results. A dilution of PBMAs of at least 1:8 was necessary to overcome matrix interference. This resulted in calculated detection limits of 0.4 µg/L (AFB1), 2 µg/L (STC), 0.08 µg/L (OTA), 16 µg/L (DON), and 0.4 µg/L (T-2/HT-2). After analysis of 54 PBMA products from German retail stores, positive results in at least one test system were obtained for 23 samples. However, most positive results were near the calculated detection limit. Control analyses of selected samples by LC–MS/MS for AFB1, STC, and OTA qualitatively confirmed the presence of trace amounts of STC in some samples, but quantitative agreement was poor. It was concluded that the high diversity of ingredients used in PBMAs led to a highly variable degree of sample matrix interference even in a 1:8 dilution. Since the use of higher dilutions conflicts with the need to achieve low detection limits, the application of EIA for routine mycotoxin analysis in PBMA for mycotoxins requires further study on the development of a feasible sample preparation method.

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Section: Methodsmentioning

confidence: 99%

Enzyme immunoassays for the detection of mycotoxins in plant-based milk alternatives: pitfalls and limitations

et al. 2022

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“…Herein, based on the high selectivity of MIPs and the fluorescence properties of the upconversion nanoparticles, MIPs with both specificity and fluorescent signals are fabricated to recognize trace sterigmatocystin (ST) with high selectivity and sensitivity. Sterigmatocystin (ST), a secondary metabolite produced by fungi, appeared as the significant contaminates of grains and feeds, with potential carcinogenic, teratogenic, and mutagenic risks [36,37,38,39,40,41]. The analytical performance of the conventional methods for determination of ST, including high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS), was insufficient in terms of speed, simplicity, sensitivity, as well as the specificity against a complex sample matrix [42,43,44,45,46].…”

Section: Introductionmentioning

confidence: 99%

Upconversion Nanophosphor-Involved Molecularly Imprinted Fluorescent Polymers for Sensitive and Specific Recognition of Sterigmatocystin

et al. 2017

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Abstract:Originated from the bottom-up synthetic strategy, molecularly imprinted polymers (MIPs) possess the inherent ability of selective and specific recognition and binding of the target analytes, with their structural cavities that can match the target molecules in respect to size, shape, and functional groups. Herein, based on the high selectivity of MIPs and the fluorescence properties of the β-NaYF 4 :Yb 3+ , Er 3+ upconversion nanoparticles, MIPs with both specificity and fluorescent signals are fabricated to recognize trace sterigmatocystin (ST) with high selectivity and sensitivity. The structure analogue of ST, 1,8-dihydroxyanthraquinone (DT), was employed as the template molecule, acrylamide as the functional monomer, 3-methacryloyloxypropyltrimethoxysilane as the crosslinking agent, and a new molecular imprinting technique of non-aqueous sol-gel method is used to synthesize a molecularly imprinted material with high selectivity to ST. Under optimal conditions, the fluorescence enhancement of fluorescent MIPs increased as the concentration of ST increased. In the range of 0.05-1.0 mg L −1 , fluorescence enhancement and the concentration showed a good linear relationship with a detection limit of 0.013 mg L −1 . Real sample analysis achieved the recoveries of 83.8-88.8% (RSD 5.1%) for rice, 82.1-87.5% (RSD 4.6%) for maize, and 80.6-89.2% (RSD 3.0%) for soybeans, respectively, revealing the feasibility of the developed method.

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“…Sterigmatocystin (ST), a carcinogenic mycotoxin, is a secondary metabolite produced mainly by Aspergillus versicolor , Aspergillus flavus , Aspergillus nidulans , fine wrinkles and other fungi Aspergillus [1,2,3]. With a basic structure composed of two furan rings with oxygen hetero anthraquinone, ST possess a similar structure to Aflatoxin B1 and its toxicity is second only to Aflatoxin that can induce liver cancer, lung cancer and other cancers [4,5].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Core–Shell Magnetic Molecularly Imprinted Polymers for Solid-Phase Extraction and Determination of Sterigmatocystin in Food

et al. 2017

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Magnetic molecularly imprinted polymers (MMIPs), combination of outstanding magnetism with specific selective binding capability for target molecules, have proven to be attractive in separation science and bio-applications. Herein, we proposed the core-shell magnetic molecularly imprinted polymers for food analysis, employing the Fe 3 O 4 particles prepared by co-precipitation protocol as the magnetic core and MMIP film onto the silica layer as the recognition and adsorption of target analytes. The obtained MMIPs materials have been fully characterized by scanning electron microscope (SEM), Fourier transform infrared spectrometer (FT-IR), vibrating sample magnetometer (VSM), and re-binding experiments. Under the optimal conditions, the fabricated Fe 3 O 4 @MIPs demonstrated fast adsorption equilibrium, a highly improved imprinting capacity, and excellent specificity to target sterigmatocystin (ST), which have been successfully applied as highly efficient solid-phase extraction materials followed by high-performance liquid chromatography (HPLC) analysis. The MMIP-based solid phase extraction (SPE) method gave linear response in the range of 0.05-5.0 mg·L −1 with a detection limit of 9.1 µg·L −1 . Finally, the proposed method was used for the selective isolation and enrichment of ST in food samples with recoveries in the range 80.6-88.7% and the relative standard deviation (RSD) <5.6%.

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A highly specific competitive direct enzyme immunoassay for sterigmatocystin as a tool for rapid immunochemotaxonomic differentiation of mycotoxigenicAspergillusspecies

Cited by 5 publications

References 19 publications

Enzyme immunoassays for the detection of mycotoxins in plant-based milk alternatives: pitfalls and limitations

Enzyme immunoassays for the detection of mycotoxins in plant-based milk alternatives: pitfalls and limitations

Upconversion Nanophosphor-Involved Molecularly Imprinted Fluorescent Polymers for Sensitive and Specific Recognition of Sterigmatocystin

Preparation and Evaluation of Core–Shell Magnetic Molecularly Imprinted Polymers for Solid-Phase Extraction and Determination of Sterigmatocystin in Food

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