A highly specific pathogen‐responsive promoter element from the immediate‐early activated <i>CMPG1</i> gene in <i>Petroselinum crispum</i>

Kirsch, Christoph; Logemann, Elke; Lippok, Bernadette; Schmelzer, Elmon; Hahlbrock, Klaus

doi:10.1046/j.1365-313x.2001.01015.x

Cited by 84 publications

(74 citation statements)

References 45 publications

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“…On the other hand, CaPUB1 shared a relatively low degree of sequence identity with Arabidopsis AtPUB24 (At3g11840; 32% identity), tobacco Avr9/Cf-9 elicited protein 74 (GenBank accession no. AAP03884; 34% identity), and parsley (Petroselinum crispum) CMPG1 (31% identity; Kirsch et al, 2001), consistent with the notion that U-box proteins are encoded by a large multigene family (Azevedo et al, 2001;Mudgil et al, 2004). As was found in other U-boxcontaining proteins, CaPUB1 possessed a single U-box domain near the N-terminal region.…”

Section: Isolation and Identification Of Full-length Capub1 Cdnasupporting

confidence: 78%

“…The sequences of U-box motifs in hot pepper CaPUB1, Arabidopsis AtPUB22, AtPUB23, and AtPUB24, tobacco ACRE74 (GenBank accession no. AAP03884), parsley CMPG1 (Kirsch et al, 2001), rice SPL11 (Zeng et al, 2004), and human hCHIP and Prp19 (Ohi et al, 2003) are shown. Amino acid residues that are conserved in at least seven of the nine sequences are shaded.…”

Section: Isolation and Identification Of Full-length Capub1 Cdnamentioning

confidence: 99%

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Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

et al. 2006

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The U-box motif is a conserved domain found in the diverse isoforms of E3 ubiquitin ligase in eukaryotes. From water-stressed hot pepper (Capsicum annuum L. cv Pukang) plants, we isolated C. annuum putative U-box protein 1 (CaPUB1), which encodes a protein containing a single U-box motif in its N-terminal region. In vitro ubiquitination and site-directed mutagenesis assays revealed that CaPUB1 possessed E3 ubiquitin ligase activity and that the U-box motif was indeed essential for its enzyme activity. RNA gel-blot analysis showed that CaPUB1 mRNA was induced rapidly by a broad spectrum of abiotic stresses, including drought, high salinity, cold temperature, and mechanical wounding, but not in response to ethylene, abscisic acid, or a bacterial pathogen, suggesting its role in the early events in the abiotic-related defense response. Because transgenic work was extremely difficult in hot pepper, in this study we overexpressed CaPUB1 in Arabidopsis (Arabidopsis thaliana) to provide cellular information on the function of this gene in the development and plant responses to abiotic stresses. Transgenic Arabidopsis plants that constitutively expressed the CaPUB1 gene under the control of the cauliflower mosaic virus 35S promoter had markedly longer hypocotyls and roots and grew more rapidly than the wild type, leading to an early bolting phenotype. Microscopic analysis showed that 35STCaPUB1 roots had increased numbers of small-sized cells, resulting in disordered, highly populated cell layers in the cortex, endodermis, and stele. In addition, CaPUB1-overexpressing plants displayed increased sensitivity to water stress and mild salinity. These results indicate that CaPUB1 is functional in Arabidopsis cells, thereby effectively altering cell and tissue growth and also the response to abiotic stresses. Comparative proteomic analysis showed that the level of RPN6 protein, a non-ATPase subunit of the 26S proteasome complex, was significantly reduced in 35STCaPUB1 seedlings as compared to the wild type. Pull-down and ubiquitination assays demonstrated that RPN6 interacted physically with CaPUB1 and was ubiquitinated in a CaPUB1-dependent manner in vitro. Although the physiological function of CaPUB1 is not yet clear, there are several possibilities for its involvement in a subset of physiological responses to counteract dehydration and high-salinity stresses in transgenic Arabidopsis seedlings.

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Section: Isolation and Identification Of Full-length Capub1 Cdnasupporting

confidence: 78%

Section: Isolation and Identification Of Full-length Capub1 Cdnamentioning

confidence: 99%

Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

et al. 2006

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“…To date, several U-box proteins have been shown to be involved in plant defense against biotic and abiotic stresses ( Table 1). The transcripts of U-box genes CMPG1 and CMPG2 were shown to accumulate extremely rapidly in parsley cell cultures after elicitor treatment, and the accumulation was localized around the pathogen infection sites in plants inoculated with Phythophthora sojae [66]. Arabidopsis homologs of CMPG1 and CMPG2 showed a similar expression pattern after challenged by elicitors [67].…”

Section: U-box-type E3 Ligases Are Newly Identified Members Of the Ubmentioning

confidence: 95%

Ubiquitination-mediated protein degradation and modification: an emerging theme in plant-microbe interactions

et al. 2006

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Post-translational modification is central to protein stability and to the modulation of protein activity. Various types of protein modification, such as phosphorylation, methylation, acetylation, myristoylation, glycosylation, and ubiquitination, have been reported. Among them, ubiquitination distinguishes itself from others in that most of the ubiquitinated proteins are targeted to the 26S proteasome for degradation. The ubiquitin/26S proteasome system constitutes the major protein degradation pathway in the cell. In recent years, the importance of the ubiquitination machinery in the control of numerous eukaryotic cellular functions has been increasingly appreciated. Increasing number of E3 ubiquitin ligases and their substrates, including a variety of essential cellular regulators have been identified. Studies in the past several years have revealed that the ubiquitination system is important for a broad range of plant developmental processes and responses to abiotic and biotic stresses. This review discusses recent advances in the functional analysis of ubiquitination-associated proteins from plants and pathogens that play important roles in plant-microbe interactions.

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“…Again, all relevant materials and methods, including treatments with UV light (20) or with the P. sojae-derived synthetic peptide elicitor, PEP25 (11,19), used here at 0.33 g/ml, have been described. Each assay contained 5 g Sca-linearized plasmid per Ϸ2 ϫ 10 6 parsley protoplasts.…”

Section: Methodsmentioning

confidence: 99%

Crosstalk among stress responses in plants: Pathogen defense overrides UV protection through an inversely regulated ACE/ACE type of light-responsive gene promoter unit

Logemann

Hahlbrock

2002

Proc. Natl. Acad. Sci. U.S.A.

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Plants often have to cope with two or more environmental hazards simultaneously. Such coincidences require instantaneous decisions on relative severity and consequential crosstalk between the respective signaling cascades. Among the frequently encountered threats are pathogen infections and UV irradiation, both of which trigger specifically targeted defense responses by means of changes in gene transcription rates. In Petroselinum crispum, pathogen defense has been shown to be associated with extensive metabolic reprogramming, including strong repression of the UVprotective flavonoid biosynthetic pathway. Here we show that one of the involved genes, encoding acyl-CoA oxidase, responds positively to UV light and negatively to a pathogen-derived elicitor through an inversely regulated promoter unit consisting of two almost identical ACGT-containing elements (ACEs). This unit, when either introduced into an unrelated promoter or generated by mutation of a differently composed unit, confers the same type of response pattern on the recipient genes, confirming its general functionality at a convergence site of two largely distinct signaling pathways. Similarly large, rapid, and partly inverse effects of UV light and elicitor were observed for several mRNAs encoding common plant regulatory factors (CPRFs) that exhibit distinct dimerization and DNA-binding properties. This striking coincidence suggests a major role of common plant regulatory factors in mediating the apparent switch in the function of ACGTcontaining elements from positive UV light to negative elicitor or pathogen responsiveness.

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A highly specific pathogen‐responsive promoter element from the immediate‐early activated CMPG1 gene in Petroselinum crispum

Cited by 84 publications

References 45 publications

Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

Ubiquitination-mediated protein degradation and modification: an emerging theme in plant-microbe interactions

Crosstalk among stress responses in plants: Pathogen defense overrides UV protection through an inversely regulated ACE/ACE type of light-responsive gene promoter unit

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