A histochemical demonstration of calcium in the maturation stage enamel organ of rat incisors.

Takano, Yoshiro; Matsuo, Sadashige; Wakisaka, Satoshi; Ichikawa, Hiroyuki; Nishikawa, S.; Akai, M.

doi:10.1679/aohc.51.241

Cited by 17 publications

(12 citation statements)

References 32 publications

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“…To observe reactive structures more in detail, Takano et al (1988) applied rapid freezing, freeze substitution, and resin embedding to this original method. Although the resulting resin sections can be used for transmission electron microscopy as well as light microscopy, the preparation takes several days from freezing to sectioning.…”

Section: Discussionmentioning

confidence: 99%

“…Calcium was detected by glyoxal bis(2-hydroxyanil) (GBHA) staining that visualizes calcium 6 as red calcium-GBHA complex in histological sections (Kashiwa 1966;Takano et al 1988). The procedures conformed to Takano et al (1988).…”

Section: Calciummentioning

confidence: 99%

“…The procedures conformed to Takano et al (1988). Sections were stained with 5% GBHA (Fluka, Switzerland) in 75% ethanol containing 3.4% NaOH for 5 min at room temperature.…”

Section: Calciummentioning

confidence: 99%

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Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Yamamoto

Domon

Takahashi

et al. 2006

Histochem Cell Biol

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This study was designed to detect tissue non-specific alkaline phosphatase (TNSALP) by Azo-dye staining, calcium by glyoxal bis(2-hydroxyanil) (GBHA) staining, bone sialoprotein (BSP) and osteopontin (OPN) by immunoperoxidase staining in developing rat molars, and also to discuss the mineralization process during acellular cementogenesis. To restrain a reduction in histochemical and immunohistochemical reactions, fresh-frozen undemineralized sections were prepared. Where the epithelial sheath was intact, TNSALP reaction was observed in the dental follicle, but not in the epithelial sheath. With the onset of dentin mineralization, the BSP-and OPN-immunoreactive, initial cementum layer appeared. At this point cementoblasts had shown intense TNSALP reaction and GBHA reactive particles (=calcium-GBHA complex) appeared on the root surface. With further development, the reaction of TNSALP and GBHA became weak on the root surface. Previous studies have shown that the initial cementum is fibril-poor and that matrix vesicles and calciferous spherules appear on the root surface only during the initial cementogenesis. The findings mentioned above suggest that: During the initial cementogenesis cementoblasts release matrix vesicles which result in calciferous spherules, corresponding to the GBHA reactive particles. The calciferous spherules trigger the mineralization of the initial cementum. After principal fiber attachment mineralization advances along collagen fibrils without matrix vesicles.

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Section: Discussionmentioning

confidence: 99%

Section: Calciummentioning

confidence: 99%

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Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Yamamoto

Domon

Takahashi

et al. 2006

Histochem Cell Biol

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“…Furthermore, because of the inherent limitations associated with chemical fixation, it is important that the current understanding of the ultrastructure and organization of ameloblasts be verified and confirmed by cryostabilization methods, generally believed to preserve tissues close to the living state (Gilkey and Staehelin, 1986;Knoll et al, 1987;Kellenberger et al, 1992). Despite numerous morphological and cytochemical studies using conventional approaches, there are only few reports on the application of cryoprocessing to examine ameloblasts at the ultrastructural level (mainly related to calcium handling by these cells; Takano et al, 1990;Takano, 1992). An important technical consideration for the cryofixation of ameloblasts is that isolation and dissection of teeth in order to expose these cells may result in substantial mechanical damage.…”

mentioning

confidence: 99%

Ultrastructural studies and immunolocalization of enamel proteins in rodent secretory stage ameloblasts processed by various cryofixation methods

1994

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“…Many researchers in the field of tooth morphogenesis agree that the maturation ameloblasts and the papillary layer including the vascular network pursuit a role as a functional unit related to the removal of water and the organic materials from the maturing enamel and transfer of minerals into the enamel (Kallenbach 1966;Garant 1972;Takano and Ozawa 1980;Sasaki and Higashi 1983;Takano et al 1988). Salivary gland duct cells (Topilko and Caillou 1985A), intestinal epithelium (Sine et al 1991), and vascular endothelial cells (Lan et al 1996) are some of the examples for fully differentiated non-neuronal cells, which show ChE activity, each functioning mainly in ion exchange and absorption.…”

Section: Discussionmentioning

confidence: 99%

Histochemical localization of cholinesterase activity in the dental epithelium of guinea pig teeth

Jayawardena

Takano

2004

Anat Embryol

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Cholinesterase is known for its remarkable diversity in distribution and function. An association of this enzyme with proliferative and morpho-differentiating tissues has been reported in several species. Here we report on the first evidence of the presence of cholinesterase in the enamel organ of continuously erupting incisors and molars of the guinea pig. Frozen sections of the incisors and molars of the guinea pig were incubated for histochemical demonstration of cholinesterase activity by means of the thiocholine method as described by Karnovsky and Root. The cholinesterase activity was observed in several types of cells of the dental epithelium; cells forming the basal portion of the enamel organ, outer enamel epithelium and maturation stage ameloblasts of both the incisors and molars. In the crown analogue side, the outer enamel epithelial cells gained strong reactions for cholinesterase and maintained the reaction throughout the secretory and maturation stages of amelogenesis. In contrast, cholinesterase reactions were lacking in the inner enamel epithelium, pre-ameloblasts, and secretory ameloblasts. In the early stage of enamel maturation, ameloblasts began to show positive reactions for cholinesterase, which was upregulated in the incisal direction. Although both tooth types showed similar reactive patterns for cholinesterase at the growing ends, maturation ameloblasts depicted a different pattern of staining displaying the reactions only sporadically in molars. These data indicate the role of cholinesterase in the enamel organ in tooth morphogenesis and function of guinea pig teeth.

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A histochemical demonstration of calcium in the maturation stage enamel organ of rat incisors.

Cited by 17 publications

References 32 publications

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Ultrastructural studies and immunolocalization of enamel proteins in rodent secretory stage ameloblasts processed by various cryofixation methods

Histochemical localization of cholinesterase activity in the dental epithelium of guinea pig teeth

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