A histological, immunohistochemical and biochemical study of the effects of pomegranate peel extracts on gibberellic acid induced oxidative stress in adult rat testes

Khalaf, Hanaa A; Arafat, Eetmad A.; Ghoneim, Fatma M

doi:10.1080/10520295.2019.1602884

Cited by 11 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This support our finding concerning the α-smooth muscle actin (α-SMA) in which NAC reversed the TGF-β1-stimulated α -SMA expression (Meurer et al, 2005) also, Kopp et al (2006) proposed that NAC repeals TGFβ1 signaling and consequent fibrogenesis adjective of proteins related with Dupuytren's disease. Our finding revealed that GA3 treated group shows a significant increase of TNFα and NFkB, this result was in agreement with (Khalaf et al, 2019). Our data revealed that GA3…”

Section: Discussionsupporting

confidence: 91%

Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity

et al. 2021

View full text Add to dashboard Cite

The extensive usage of gibberellic acid (GA3) in agriculture and plant growth is generally associated with enormous human and public health hazards. The present research assesses the impact of n-acetyl cysteine (NAC) on the hepatorenal injury persuaded by GA3 for this purpose, After two weeks of adaptation twenty-four rats allocated into four groups (6 rats/group) as follows: control group, supplied with saline only; n-acetyl cysteine (NAC) group, provided with 150 mg/kg/bw by stomach tube (orally) dissolved in saline; Positive GA3 group, received GA3 (55 mg/kg/bw) orally; Protective group received NAC (150 mg/kg/bw) and GA3 (55 mg/kg/bw) as in NAC and GA3 groups. Rats received their treatments for consecutive 3 weeks.On day 22, rats were anesthetized, then euthanized. Blood and tissue samples were obtained for biochemical, antioxidants markers analysis, gene expression, and histopathological examination. Our results revealed significant changes in serum AST, ALT, urea, uric acid, total protein, and albumin levels with a substantial rise of MDA and NO concentration in GA3 treated rats along with a considerable decrease of the GSH and overexpression of the inflammatory hepatic and renal cytokines (IL-10, TNFα, NOS) and fibrotic gene expression TGF-β1, and α-SMA, with boost expression of nuclear factor-kappa (NF k B). NAC co-administered with GA3 significantly normalized the kidney and liver function and the antioxidant state, besides normal histological structure of both liver and kidney tissue and downregulated expression of the proinflammatory cytokines as well as, fibrogenic gene expression. How to cite this article: Soliman MM, Aldhahrani A, Gaber A, et al. Impacts of n-acetyl cysteine on gibberellic acid-induced hepatorenal dysfunction through modulation of proinflammatory cytokines, antifibrotic and antioxidant activity. J

show abstract

Section: Discussionsupporting

confidence: 91%

Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The increased testicular SOD activity by PP is similar to (Dkhil et al., 2013; Khalaf et al., 2019) where PP was more potent antioxidant than pomegranate juice. The potent antioxidant activity of PP explains its enhancement effect on semen quality.…”

Section: Discussionsupporting

confidence: 81%

“…Pomegranate and Aloe Vera were formerly reported to possess antioxidant activity, that is variable according to the type of extract and part of the plant used (Avdatek et al., 2018; Khalaf, Arafat, & Ghoneim, 2019; Zeweil, Elnagar, Zahran, Ahmed, & El‐Gindy, 2013). Pomegranate peels were found to possess more potent antioxidant properties than pomegranate juice (Dkhil, Al‐Quraishy, & Moneim, 2013; Zeweil et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary pomegranate peel (Punica granatum L.) and Aloe vera gel (Aloe barbadensis miller) supplementation on testicular antioxidant biomarkers and spermatogenesis enzymes in mature V‐Line rabbit bucks

Bakeer

El-Attrouny

Abdelatty

2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

Rabbit meat is considered as an economic source of white meat, increasing its production is limited by the buck fertility, since one rabbit can be used to inseminate up to 15 female. The aim of the current study is to enhance male rabbit fertility by using dietary antioxidants including Aloe vera gel (AVG) and pomegranate peels (PP). In a 60 days experiment, 48 V‐Line 5‐month‐old rabbit bucks of average body weight (2,300 ± 20) kg were allocated into four dietary treatments (n = 12/group) as follow: CON (fed on control diet), ALOE (received AVG in drinking water; 500 mg/L drinking water), POM3 (fed on basal diet + 3% of pomegranate) and POM5 (fed on basal diet + 5% of pomegranate). Semen samples were collected at d30 and d60 of the experiment and used for analysis of semen quality. Sexual behaviour was reported in terms of latency to first mating and ejaculation interval. At the end of the experiment, six bucks were euthanized from each group, blood samples were collected and used for testosterone level determination and testicular tissue samples were collected and used for key antioxidant and spermatogenesis enzymes assessment, and testes histopathological evaluation. The UNIVARIATE procedures of SAS 9.4 were used to analyse the data, significance was declared at p ≤.05. PP supplementation improved percentage of progressive motile sperms while AVG negatively impacted it (p = .04), sperm concentration and metabolically active sperm cells were the highest in PP and lowest in ALOE supplemented bucks (p = .01 and .01; respectively). Testicular alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) decreased in AVG supplemented group (p = .01 and .02; respectively). From our findings, AVG in its fresh form decreased fertility of rabbit bucks, while PP is potent fertility boosting for rabbit bucks.

show abstract

“…Our data are in agreement with those of other studies (Soliman et al, 2021b) that revealed a decrease in the activity of antioxidants in adult rats (Khalaf et al, 2019). GSH and SOD are oxidative stress biomarkers that are required for testes integrity (H. Yang et al, 2011).…”

Section: Discussionsupporting

confidence: 93%

Chrysin abrogates gibberellic acid‐induced testicular oxidative stress and dysfunction via the regulation of antioxidants and steroidogenesis‐ and apoptosis‐associated genes

Soliman

Aldhahrani

Ghamry

et al. 2022

Journal of Food Biochemistry

View full text Add to dashboard Cite

GA3 is widely used as a growth stimulant in agricultural regions. The long‐term use of GA3 can cause organs damage. Chrysin is a flavonoid found in nature that is commonly used to treat organ toxicity. In this study, we examined the effect of chrysin on the testes function of GA3‐affected rats. A total of 24 male Wistar rats were divided into 4 groups. Saline was given to the control group. The chrysin group was given orally 50 mg/kg/BW of chrysin in saline. The GA3 group received a daily oral gavage of GA3 (55 mg/kg/BW). The protective group (chrysin + GA3) was given chrysin and GA3 as those described in chrysin and GA3 groups. There were an increase in MDA levels in the serum and testicular tissue of GA3‐treated group. Catalase, GSH, and SOD levels were all lowered in the GA3‐treated rats. Chrysin dramatically reduced the harmful effects of GA3 by restoring reproductive hormone levels, altered sperm parameters, and antioxidant capabilities. Furthermore, GA3 reduced the quantitative expression of steroidogenesis genes StAR and 3‐HSD, as well as Bcl2 genes, while it increased the apoptotic marker BAX; all were alleviated by the pre‐administration of chrysin. The pre‐administration of chrysin protected the GA3 group from spermatogenic vacuolation, interstitial edema, necrosis, and depletion. Chrysin inhibited oxidative stress and modulated antioxidant activity, as well as apoptosis‐/anti‐apoptosis‐related mediators in the testes. Chrysin has the potential to repair GA3‐induced testicular dysfunctions. This suggests that chrysin is preferable as a medication to mitigate GA3‐induced oxidative damage in the testes. Practical applications Chrysin has the potential to repair GA3‐induced testicular dysfunctions. This suggests that chrysin is preferable as a medication to mitigate GA3‐induced oxidative damage in the testes.

show abstract

A histological, immunohistochemical and biochemical study of the effects of pomegranate peel extracts on gibberellic acid induced oxidative stress in adult rat testes

Cited by 11 publications

References 42 publications

Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity

Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity

Effect of dietary pomegranate peel (Punica granatum L.) and Aloe vera gel (Aloe barbadensis miller) supplementation on testicular antioxidant biomarkers and spermatogenesis enzymes in mature V‐Line rabbit bucks

Chrysin abrogates gibberellic acid‐induced testicular oxidative stress and dysfunction via the regulation of antioxidants and steroidogenesis‐ and apoptosis‐associated genes

Contact Info

Product

Resources

About