A Histone Code Reader and a Transcriptional Activator Interact to Regulate Genes for Salt Tolerance

Wei, Wei; Tao, Jian-Jun; Chen, Haowei; Li, Qingtian; Zhang, Wan–Ke; Ma, Biao; Lin, Qing; Zhang, Jinsong; Chen, Shouyi

doi:10.1104/pp.16.01764

Cited by 55 publications

(49 citation statements)

References 68 publications

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“…The main roots were cut and the seedlings were moved to water until the hairy roots grew to 10 cm. Then, the seedlings were transferred to 150 m m mannitol for 24 h. All the trifoliates were cut and dried at 65°C for 2 days to measure water content: RWC (%) = (water content after salt treatment)/(water content in water) (Kereszt et al ., ; Wei et al ., ).…”

Section: Methodsmentioning

confidence: 97%

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GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

et al. 2019

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† These authors contributed equally to this work. SUMMARYWRKY transcription factors play important roles in response to various abiotic stresses. Previous study have proved that soybean GmWRKY54 can improve stress tolerance in transgenic Arabidopsis. Here, we generated soybean transgenic plants and further investigated roles and biological mechanisms of GmWRKY54 in response to drought stress. We demonstrated that expression of GmWRKY54, driven by either a constitutive promoter (pCm) or a drought-induced promoter (RD29a), confers drought tolerance. GmWRKY54 is a transcriptional activator and affects a large number of stress-related genes as revealed by RNA sequencing. Gene ontology (GO) enrichment and co-expression network analysis, together with measurement of physiological parameters, supported the idea that GmWRKY54 enhances stomatal closure to reduce water loss, and therefore confers drought tolerance in soybean. GmWRKY54 directly binds to the promoter regions of genes including PYL8, SRK2A, CIPK11 and CPK3 and activates them. Therefore GmWRKY54 achieves its function through abscisic acid (ABA) and Ca 2+ signaling pathways. It is valuable that GmWRKY54 activates an ABA receptor and an SnRK2 kinase in the upstream position, unlike other WRKY proteins that regulate downstream genes in the ABA pathway. Our study revealed the role of GmWRKY54 in drought tolerance and further manipulation of this gene should improve growth and production in soybean and other legumes/crops under unfavorable conditions.

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Section: Methodsmentioning

confidence: 97%

“…Conductivity (K2) was measured again until water temperature dropped to room temperature. Relative EL = K1/K2, and five plants of each transgenic and null line were measured (Wei et al ., ).…”

Section: Methodsmentioning

confidence: 97%

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

et al. 2019

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“…PHD proteins affect the processes of growth and development by changing transcription and reading epigenetic histone modifications, but their functions in abiotic stress responses remain largely unclear. Transgenic Arabidopsis plants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants [31,50].…”

Section: Identification Of Ghphd Respond To Abiotic Stresses and Varimentioning

confidence: 98%

Response of phytohormone mediated plant homeodomain (PHD) family to abiotic stress in upland cotton (Gossypium hirsutum spp.)

Zheng

Qanmber

et al. 2020

Preprint

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Background The sequencing and annotations of cotton genomes provide strong theoretical support to reveal more physiological phenomena and functions. Plant homeodomain (PHD) protein family have been reported to be involved in regulating diverse biological processes in plants. However, their functions have not yet been carried out in cotton. Results In this study, we performed a genome-wide analysis of the PHD genes in cotton, including the chromosomal location, phylogenetic relationship, gene structure, and conserved domains. Using a phylogenetic analysis, we divided the 297 PHD genes into five subgroups. The GhPHDs were unevenly distributed across all 26 chromosomes in upland cotton, and whole genome duplication events analyses showed that purifying selection might contributed greatly to the maintenance of function in the GhPHD family. Expression pattern analysis based on RNA-seq data and qRT-PCR results showed that the most of GhPHD genes have significant tissue-specific spatial and temporal expression patterns, indicating GhPHD have multiple functions in growth and development. We further summarized the cis -acting elements in response to abiotic stresses and plant hormones, and treated cotton seedlings with abiotic stresses and plant hormones, respectively. Then, GhPHD gene expression level were detected by qRT-PCR, which indicated that GhPHD could response to stresses and plant hormones. Co-expression network analysis also indicated that GhPHDs were essential for plant growth and development, and phytohormone mediate GhPHD response to abiotic stress can improve plant tolerance to adverse environment. Conclusion This study provides useful information to facilitate the further study of the function of the GhPHD family.

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“…The pCAMBIA3301-miR398c vector was used as an overexpression vector. The hypocotyls of 5-day-old soybean seedlings were injected with Agrobacterium rhizogenes (K599) harboring the pCas9-U6-sgRNAs or pCAMBIA3301-miR398c plasmid [50]. The seedlings were cultivated in humidity for 6 days, after which the top leaves were removed and the wound sites were covered with vermiculite for about 15 days until hairy roots about 10 cm in length had developed ( Figure S8).…”

Section: Agrobacterium Rhizogenes-mediated Soybean Hairy Roots Transfmentioning

confidence: 99%

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

Zhou

Liu

et al. 2019

Preprint

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Background: Drought conditions adversely affect soybean growth, resulting in severe yield losses worldwide. Increasing experimental evidence indicates miRNAs are important post-transcriptional regulators of gene expression. However, the drought-responsive molecular mechanism underlying miRNA–mRNA interactions remains largely uncharacterized in soybean. Meanwhile, the miRNA-regulated drought response pathways based on multi-omics approaches remain elusive. Results: We combined sRNA, transcriptome and degradome sequencing to elucidate the complex regulatory mechanism mediating soybean drought resistance. One-thousand transcripts from 384 target genes of 365 miRNAs, which were enriched in the peroxisome, were validated by degradome-seq. An integrated analysis showed 42 miRNA–target pairs exhibited inversely related expression profiles. Among these pairs, a strong induction of gma-miR398c as a hub gene negatively regulates multiple peroxisome-related genes ( GmCSD 1a/b, GmCSD 2a/b/c and GmCCS ). Meanwhile, we detected that alternative splicing of GmCSD1a/b might affect soybean drought tolerance by bypassing gma-miR398c regulation. Overexpressing gma-miR398c in Arabidopsis thaliana L. resulted in decreased percentage germination, increased leaf water loss, and reduced survival under water deficiency, which displayed sensitivity to drought during seed germination and seedling growth. Furthermore, overexpressing gma-miR398c in soybean decreased GmCSD 1a/b, GmCSD 2a/b/c and GmCCS expression, which weakened the ability to scavenge O 2 .− , resulting in increased relative electrolyte leakage and stomatal opening compared with knockout miR398c and wild-type soybean under drought conditions. Conclusion: The study indicates that gma-miR398c negatively regulates soybean drought tolerance, and provides novel insights useful for breeding programs to improve drought resistance by CRISPR technology.

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A Histone Code Reader and a Transcriptional Activator Interact to Regulate Genes for Salt Tolerance

Cited by 55 publications

References 68 publications

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

Response of phytohormone mediated plant homeodomain (PHD) family to abiotic stress in upland cotton (Gossypium hirsutum spp.)

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

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A Histone Code Reader and a Transcriptional Activator Interact to Regulate Genes for Salt Tolerance

Cited by 55 publications

References 68 publications

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca2+ signaling pathways in transgenic soybean

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca2+ signaling pathways in transgenic soybean

Response of phytohormone mediated plant homeodomain (PHD) family to abiotic stress in upland cotton (Gossypium hirsutum spp.)

Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

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GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean