A histone deacetylase gene, SlHDA3, acts as a negative regulator of fruit ripening and carotenoid accumulation

Abstract

SlHDA3 functions as an inhibitor and regulates tomato fruit ripening and carotenoid accumulation. Post-translational modifications, including histones acetylation, play a pivotal role in the changes of chromatin structure dynamic modulation and gene activity. The regulation of histone acetylation is achieved by the action of histone acetyltransferases and deacetylases, which play crucial roles in the regulation of transcription activation. There is an increasing research focus on histone deacetylation in crops, but the role of histone deacetylase genes (HDACs) in tomato has not been elucidated. With the aim of characterizing the tomato RPD3/HDA1 family histone deacetylase genes, SlHDA3 was isolated and its RNA interference (RNAi) lines was obtained. The fruit of SlHDA3 RNAi lines exhibited accelerated ripening process along with short shelf life characteristics. The accumulation of carotenoid was increased due to the alteration of the carotenoid pathway flux. Climacteric ethylene production also stimulated along with significantly up-regulated expression of ethylene biosynthetic genes (ACS2, ACS4, ACO1 and ACO3) and fruit ripening-associated genes (RIN, E4, E8, PG, Pti4, LOXB, Cnr and TAGL1) in SlHDA3 RNAi lines. Besides, fruit cell wall metabolism-associated genes (HEX, MAN, TBG4, XTH5 and XYL) were enhanced in transgenic lines. Relative to wild type (WT) plants, SlHDA3 RNAi seedlings displayed shorter hypocotyls and more sensitivity to ACC (1-aminocyclopropane-1-carboxylate). These results indicated that SlHDA3 is involved in the regulation of fruit ripening by affecting ethylene biosynthesis and carotenoid accumulation.

Transcriptome analysis provides new insight into the molecular mechanisms in regulating carotenoid accumulation in pericarp of fruits from two pummelo cultivars Citrus grandis (L.) Osbeck

“…SlHDA3, a member of tomato RPD3/HDA1 family of histone deacetylases, played a negative role in regulating carotenoid accumulation in tomato fruits [87]. It can be ovbserved that members from different histone deacetylase families may paly contradictory roles in modulating carotenoid metabolic pathway.…”

Hypomethylation of the aquatic invasive plant, Ludwigia grandiflora subsp. hexapetala mimics the adaptive transition into the terrestrial morphotype

“…This addition is provided de novo and maintained by methyltransferase enzymes through three different sequence contexts in plants: CG, CHG and CHH (Meyer 2015). Over recent years, many studies have highlighted the role of DNA methylation in many biological and ecological mechanisms in plants, such as development (Guo et al 2018, responses to biotic and abiotic stressors (Tricker 2015, Crisp et al 2016, Lämke and Bäurle 2017, cellular-stress response memory and priming (Mauch-Mani et al 2017, Sow et al 2018, phenotypic plasticity (Gourcilleau et al 2010, Baulcombe and Dean 2014, Kooke et al 2015, Conde et al 2017, and possibly adaptation (Bräutigam et al 2013, Kawakatsu et al 2016, Rey et al 2016, Schmid et al 2018, Gourcilleau et al 2019. Kooke et al (2015) used 99 Epigenetic Recombinant Inbred Lines (epiRILs) of Arabidopsis thaliana, consisting of crosses between the parents Col-0 and the hypomethylated mutant lines ddm1 and ddm2, to compare the morphological responses of the plants exposed to saline conditions.…”

Genome-Wide Identification of Histone Acetyltransferases and Deacetylases in Epiphytic Orchid Plant Dendrobium Catenatum

“…The genome-wide identi cation of HATs and HDACs has been performed in a group of species: Arabidopsis thaliana (12 HATs, 18 HDACs) [5], Oryza sativa (8 HATs,18 HDACs) [6,7], Vitis vinifera (7 HATs, 13 HDACs), [8], Solanum lycopersicum (32 HATs, 15 HDACs) [9], Citrus sinensis (50 HATs, 16 HDACs) [10], Litchi chinensis (6 HATs,11 HDACs) [11], Malus domestica (57 HATs,26 HDACs) [12], Zea mays (12 HATs,16 HDACs) [12], and lower plant Marchantia polymorpha (8 HATs,12 HDACs) [13]. Additionally, There are 11 HDACs in Populus trichocarpa [14], 28 HDACs in Glycine max [15], 30 HDACs including 15 in each of the A-and D-subgenomes of Gossypium hirsutum [16], 9 HATs in either G. raimondi or G. arboretum, and 18 HATs in G. hirsutum [17].…”

“…Tomato histones acetylation status affects ethylene-dependent fruit ripening and carotenoid accumulation. Knockdown of SlHDA1 or SlHDA3 results in accelerated fruit ripening process along with short shelf life characteristics, but knockdown of SlHDT3 results in delayed ripening along with prolonged shelf life [27][28][29]. In terms of environmental stimulus and stress response, histone acetyltransferase TAF1/HAF2 and GCN5 and histone deacetylase HD1 (HDA19) are involved in light regulation of growth and gene expression [30].…”