We derive a blow-up formula for holomorphic Koszul-Brylinski homologies of compact holomorphic Poisson manifolds. As applications, we investigate the invariance of the E1-degeneracy of the Dolbeault-Koszul-Brylinski spectral sequence under Poisson blowups, and compute the holomorphic Koszul-Brylinski homology for del Pezzo surfaces and two complex nilmanifolds with holomorphic Poisson structures.Question. For a non-trivial holomorphic Poisson structure, can we describe explicitly the variance of the holomorphic Koszul-Brylinski homology under a Poisson blow-up?Using a sheaf-theoretic approach, we establish a blow-up formula for holomorphic Koszul-Brylinski homology as follows.Theorem 1.1. Suppose (X, π) is a compact holomorphic Poisson manifold of complex dimension n ≥ 2, and (Z, π| Z ) ⊂ (X, π) is a closed holomorphic Poisson submanifold of codimension c ≥ 2 with trivial transverse Poisson structure. Let ϕ : X → X be the blow-up of X along Z and π be the unique holomorphic Poisson structure on X such that ϕ is a Poisson morphism, i.e., ϕ ⋆ π = π. Then there exists an isomorphism of holomorphic Koszul-Brylinski homologies:for any 0 ≤ k ≤ 2n. In particular, there exists an isomorphismObserve that the first page of the Dolbeault-Koszul-Brylinski spectral sequence of the holomorphic Poisson manifold (X, π) is the Dolbeault cohomology:The study of the degeneracy of the Dolbeault-Koszul-Brylinski spectral sequence at E 1 -page may be of independent interest. As an application of Theorem 1.1, we investigate the invariance of such degeneracy under Poisson blow-ups.Theorem 1.2. With the assumption of Theorem 1.1, the Dolbeault-Koszul-Brylinski spectral sequence for ( X, π) degenerates at E 1 -page if and only if it does so for (X, π) and (Z, π| Z ).This paper is organized as follows. In §2, we review some basics on holomorphic Poisson manifolds and the holomorphic Koszul-Brylinski homology. We devote §3 to Poisson blow-ups and modifications. In §4 we derive the Poisson projective bundles formula for holomorphic Koszul-Brylinski homology, a key part of the proof of the main theorems. In §5, the proofs of the main theorems are given. In §6, the holomorphic Koszul-Brylinski homologies of some compact holomorphic Poisson manifolds are computed. Finally, the Appendix A gives the Hodge diamond of a six-dimensional complex nilmanifold in § §6.3.