A homogeneous fluorescence polarization assay for detection of antibody to Brucella abortus

Nielsen, Klaus; Gall, D.; Jolley, Michael E.; Leishman, G; Balsevicius, S.; Smith, Phillip D.; Nicoletti, P; Thomas, F C

doi:10.1016/0022-1759(96)00116-0

Cited by 107 publications

(85 citation statements)

References 20 publications

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“…Apesar de ainda pouco utilizadas no Brasil, onde não têm seus respectivos pontos de corte devidamente estabelecidos pelo PNCEBT, os resultados da FC e da FPA são, de modo geral , compatíveis com diagnóstico positivo de acordo com Alton et al (1988) e Nielsen et al (1996). Os altos títulos obtidos na SAL/2-ME e na FC e os valores de milipolarização demonstraram concordância entre os resultados encontrados nas diferentes provas.…”

Section: Discussionunclassified

“…(Brasil, 2006) e, posteriormente, à fixação de complemento (FC) (Alton et al, 1988) e à polarização fluorescente (FPA) (Nielsen et al, 1996), sendo utilizado o "Brucella abortus Antibody Test Kit", produzido por Diachemix, USA, e o equipamento de leitura de polarização fluorescente modelo Sentry 100® (Diachemix, USA). Uma vez reagentes ao teste confirmatório do 2-ME, os animais foram sacrificados na propriedade, conforme recomenda o PNCEBT.…”

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Confirmação de infecção por Brucella abortus em um rebanho bovino certificado livre em Minas Gerais: relato de caso

Filho

Wanderley

Faria

et al. 2012

Arq. Bras. Med. Vet. Zootec.

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Section: Discussionunclassified

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Confirmação de infecção por Brucella abortus em um rebanho bovino certificado livre em Minas Gerais: relato de caso

Filho

Wanderley

Faria

et al. 2012

Arq. Bras. Med. Vet. Zootec.

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“…An unbound antigen that has a fluorescence marker will spin at a greater speed than an antibody-bound-antigen [97]. This measurement is taken in consideration with background emittance and produces a millipolarization value that can be used to classify an animal as negative, suspect, or reactor for brucellosis.…”

Section: Diagnosticsmentioning

confidence: 99%

Comparisons of brucellosis between human and veterinary medicine

Hull

Schumaker

2018

Infection Ecology & Epidemiology

152

129

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Brucellosis is the world’s most widespread zoonosis, but also ranks as one of the seven most neglected diseases, according to the World Health Organization. Additionally, it is recognized as the world’s most common laboratory-acquired infection. There are a reported 500,000 incident cases of human brucellosis per year. However, true incidence is estimated to be 5,000,000 to 12,500,000 cases annually. Once diagnosed, focus is directed at treating individual patients with antibiotic regimes, yet overall neglecting the animal reservoir of disease. Countries with the highest incidence of human brucellosis are Syria (1,603.4 cases per 1,000,000 individuals), Mongolia (391.0), and Tajikistan (211.9). Surveillance on animal populations is lacking in many developed and developing countries. According to the World Animal Health Information Database, Mexico had the largest number of reported outbreaks, 5,514 in 2014. Mexico is followed by China (2,138), Greece (1,268), and Brazil (1,142). The majority of these outbreaks is Brucella abortus, the etiologic agent of bovine brucellosis. Brucellosis is an ancient disease that still plagues the world. There are still knowledge gaps and a need for better diagnostics and vaccines to make inroads towards control and eradication.

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“…Once the blank/background read is performed (2-3 minutes), this antigen is added to diluted serum and a measure of the antibody content is obtained in about 2 minutes after the addition of antigen using a fluorescence polarisation analyser (FPM) (Nielsen et al, 1996).…”

Section: Infection With Brucella In Pigsmentioning

confidence: 99%

“…The FPA is capable of reducing but not fully eliminating the reactions due to residual antibody produced in response to vaccination (Nielsen et al, 1996). Moreover, the specificity of FPA in FPSR conditions is currently unknown in cattle and small ruminants, but it has been clearly shown that it does not resolve the FPSR problem in swine (Praud et al, 2012).…”

Section: I)mentioning

confidence: 99%

Isolation of Brucella pinnipedialis from Grey Seals (Halichoerus grypus) in the Baltic Sea

Hirvelä-Koski

Nylund

Skrzypczak

et al. 2017

Journal of Wildlife Diseases

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Description of the disease: Brucellosis is the generic name used for the animal and human infections caused by several species of the genusCausal pathogens: Genetic and immunological evidence indicates that all members of the Brucella genus are closely related. Nevertheless, based on relevant differences in host preference and epidemiology displayed by the major variants, as well as molecular evidence of genomic variation, the International Committee on Systematics of Prokaryotes, Subcommittee on the Taxonomy of Brucella took a clear position in 2005 on a return to pre-1986 Brucella taxonomic opinion; the consequences of this statement imply the re-approval of the six classical Brucella nomenspecies with their corresponding recognised biovars. The classical names related to the six Brucella nomenspecies are validly published in the Approved Lists of Bacterial Names, 1980, and the designated type strains are attached to these validly published names: B. abortus, B. melitensis, B. suis, B. neotomae, B. ovis and B. canis 1 . The first three of these are subdivided into biovars based on cultural and serological properties (see Tables 2 and 3). Strains of Brucella have been isolated in the last decade from marine mammals but these strains cannot be ascribed to any of the above-recognised species. Investigations are continuing to establish their proper position in the taxonomy of the genus and it has been proposed that they be classified into two new species: B. ceti and B. pinnipedialis (Foster et al., 2007). A new species, named B. microti, was also isolated from the common vole (Microtus arvalis) as well as from foxes and soil in Central Europe (Scholz et al., 2008). Novel isolates from human breast implant and lung infections (strains BO1 and BO2) and from baboons that had delivered stillborn offspring have also been described, although the natural reservoir of these isolates remains unclear. While only two isolates of each new type have been described, they have been formally published as the tenth and eleventh Brucella species, B. inopinata and B. papionis respectively (Scholz et al., 2010;Whatmore et al., 2014). Finally, strains isolated from rodents, foxes and frogs were characterised as atypical Brucella strains distinct from the currently described species. They have not yet been approved as new Brucella species.Brucella is a member of the Brucellaceae family, in the order Rhizobiales, class Alphaproteobacteria. It shows close genetic relatedness to some plant pathogens and symbionts of the genera Agrobacterium and Rhizobium, as well as animal pathogens (Bartonella) and opportunistic or soil bacteria (Ochrobactrum).Infection with Brucella in cattle is usually caused by biovars (bv.) of Brucella abortus.

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A homogeneous fluorescence polarization assay for detection of antibody to Brucella abortus

Cited by 107 publications

References 20 publications

Confirmação de infecção por Brucella abortus em um rebanho bovino certificado livre em Minas Gerais: relato de caso

Confirmação de infecção por Brucella abortus em um rebanho bovino certificado livre em Minas Gerais: relato de caso

Comparisons of brucellosis between human and veterinary medicine

Isolation of Brucella pinnipedialis from Grey Seals (Halichoerus grypus) in the Baltic Sea

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