A HOPS protein, CmVps39, is required for vacuolar morphology, autophagy, growth, conidiogenesis and mycoparasitic functions of <i>Coniothyrium minitans</i>

Yang, Xiaoxiang; Hui, Chen; Chéng, Jiāsēn; Xiè, Jiǎtāo; Hsiang, Tom; Fù, Yànpíng

doi:10.1111/1462-2920.13334

Cited by 18 publications

(21 citation statements)

References 48 publications

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“…Generally, SNARE associated homotypic fusion processes are mainly regulated by homotypic fusion and protein sorting complex (HOPS) (Hong and Lev, 2014), according to Chen (2016), the HOPS complex principally consists of core subunit (Vps11, Vps16, Vps18, and Vps33) and an accessory subunit (Vps39 and Vps41). Previous research demonstrations have shown that Vps39 and Vps41 inactivity impacted negatively on vacuolar morphology and aggregation (Raymond et al, 1992; Guerra and Bucci, 2016; Medvedev et al, 2016), furthermore, the accessory subunit of the HOPS complex are crucially involved in the promotion of autophagy vegetative growth, conidiation and virulence of many filamentous fungi (Liu et al, 2006; Yang et al, 2016). Previous research findings have shown that MoVps39 a component of the HOPS complex crucially regulate conidiogenesis, appressorium formation, and development of blast infection in M. oryzae.…”

Section: Introductionmentioning

confidence: 99%

A HOPS Protein, MoVps41, Is Crucially Important for Vacuolar Morphogenesis, Vegetative Growth, Reproduction and Virulence in Magnaporthe oryzae

et al. 2017

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The homotypic fusion and protein sorting protein complex (HOPS) is the first known tether complex identified in the endocytic system that plays a key role in promoting homotypic vacuolar fusion, vacuolar biogenesis and trafficking in a wide range of organisms, including plant and fungi. However, the exact influence of the HOPS complex on growth, reproduction and pathogenicity of the economically destructive rice blast fungus has not been investigated. In this study, we identified M. oryzae vacuolar protein sorting 41 (MoVps41) an accessory subunit of HOPS complex and used targeted gene deletion approach to evaluate its contribution to growth, reproduction and infectious life cycle of the rice blast fungus. Corresponding results obtained from this study showed that MoVps41 is required for optimum vegetative development of M. oryzae and observed that MoVps41 deletion mutant displayed defective vegetative growth. Our investigation further showed that MoVps41 deletion triggered vacuolar fragmentation, compromised membrane integrity and pathogenesis of the ΔMovps41 mutant. Our studies also showed for the first time that MoVps41 plays an essential role in the regulation of sexual and asexual reproduction of M. oryzae. In summary, our study provides insight into how MoVps41 mediated vacuolar fusion and biogenesis influences reproduction, pathogenesis, and vacuolar integrity in M. oryzae and also underscores the need to holistically investigate the HOPS complex in rice blast pathogen.

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Section: Introductionmentioning

confidence: 99%

A HOPS Protein, MoVps41, Is Crucially Important for Vacuolar Morphogenesis, Vegetative Growth, Reproduction and Virulence in Magnaporthe oryzae

et al. 2017

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“…After recognizing the presence of S. sclerotiorum, C. minitans penetrates the hyphae of S. sclerotiorum with the help of fungal cell wall-degrading enzymes (fCWDEs) (14,42,43). Decreased expression levels of the exo-␤-1,3-glucanase gene Cmg and the chitinase gene CH1 have been reported in many mutants defective in parasitizing S. sclerotiorum (15,16,18). In this research, the loss of function of CmAim24 was also accompanied by the reduced expression of genes for cell wall-degrading enzymes.…”

Section: Discussionmentioning

confidence: 99%

CmAim24 Is Essential for Mitochondrial Morphology, Conidiogenesis, and Mycoparasitism in Coniothyrium minitans

Yang

Zhao

Luo

et al. 2020

Appl Environ Microbiol

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Coniothyrium minitans is an important mycoparasite of the notorious phytopathogenic fungus Sclerotinia sclerotiorum. The mycoparasitism system of C. minitans-S. sclerotiorum is unique and important in probing fungi and fungal interactions. Here, we report a conidiation-deficient mutant, ZS-1TN1961, which was screened from a transfer DNA (T-DNA) insertional library of C. minitans. A single-copy gene, encoding a protein with high sequence similarity to Aim24 (altered inheritance of mitochondria protein 24) in Saccharomyces cerevisiae, was disrupted by T-DNA insertion in this mutant. Gene replacement and complementation experiments confirmed that mutants lacking CmAim24 exhibited significantly reduced conidial production and germination as well as reduced sclerotial mycoparasitic ability. Furthermore, cellular localization assays showed that CmAim24 localized to mitochondria, and abnormal mitochondria were observed in the ΔCmAim24 mutant. The ΔCmAim24 mutant exhibited significant accumulation of reactive oxygen species (ROS) and a reduced ATP content in mycelia. In summary, our results suggest that CmAim24 plays a key role in mitochondrial architecture and function, conidiogenesis, and mycoparasitism in C. minitans. IMPORTANCE Aim24 proteins are involved in mitochondrial biogenesis and accumulate between the two membranes of a mitochondrion. Their function in prokaryotes and filamentous fungi is as yet unknown. In the present study, we characterized an Aim24 protein, CmAim24, in the mycoparasite Coniothyrium minitans and proved its critical role in mitochondrial morphology and function, conidiogenesis, conidial germination, and mycoparasitism to S. sclerotiorum.

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“…Only three orthologs in GH18were shared by C. minitans and Trichoderma spp., but they were not detected during the early stage of interaction between C. minitans and S. sclerotiorum. The chitinase gene (CMZSB_00640) and β-1,3-endoglucanase (CMZS_02526) of C. minitans are usually used as two marker genes for mycoparasitism [63], while their orthologs can also be identified in pathogenic fungi. Furthermore, these two genes and CMZSB_02023 (encoding chitinase) were also up-regulated significantly (log2 ratio > 2.5, FDR ≤ 0.001) at conidiationdata not shown).…”

Section: Discussionmentioning

confidence: 99%

Mycoparasitism illuminated by genome sequencing and digital gene expression profiling of Coniothyrium minitans, an important biocontrol fungus of the plant pathogen Sclerotinia sclerotiorum

Zhao¹,

Zhou²,

Xiè³

et al. 2019

Preprint

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BackgroundConiothyrium minitans is a mycoparasite of the notorious plant pathogen Sclerotinia sclerotiorum. To further understand the parasitism of C. minitans, here, we assembled and analyzed its genome by combining transcriptome data. ResultsThe genome of C. minitans strain ZS-1 was 39.77 Mb in 350 scaffolds. A total of 11437 predicted genes and proteins were annotated, and 30.8% of blast hits matched proteins encoded by Paraphaeosphaeria sporulosa, a worldwide soil fungus. The transcriptome of strain ZS-1 during the early interaction at 0 h, 4 h and 12 h with its host was analyzed. The detected expressed genes were involved in response to host defenses, including cell wall-degrading enzymes, transporters, secretory proteins and secondary metabolites. The fungal cell wall-degrading enzymes belonged to the GH16, GH18, and GH72 classes in CAZymes, and some were significantly up-regulated during mycoparasitism. Most of the monocarboxylate transporter genes of the major facilitator superfamily and all the detected ABC transporters, especially the heavy metal transporters, were significantly upregulated. Approximately 8% of the 11437 proteins in C. minitans were predicted to be secretory proteins, with catalytic activity, hydrolase activity, peptidase activity and serine hydrolase activity enriched in molecular function. Most genes involved in serine hydrolase activity were significantly upregulated during mycoparasitism. ConclusionThis assemble genome and genome-wide expression study demonstrate that the mycoparasitism process of C. minitans is complex and a series of genes or proteins would be deployed by C. minitans to invade successfully the host. Our study provides insights into the mechanisms of the mycoparasitism between C. minitans and S. sclerotiorum and clues to excavate active secondary metabolites from C. minitans. BackgroundConiothyrium minitans, a sclerotial mycoparasite, lives on the sclerotia of Sclerotinia sclerotiorum and its relatives in soil and was first isolated and identified in 1947 by Campbell [1]. It is distributed worldwide in America, Europe, Australia, Africa and Asia [2][3][4], and its remarkable biocontrol ability has attracted great attention. C. minitans shares similar conditions (20°C) with its host S. sclerotiorum [1,[4][5][6]. C. minitans has a narrow host range, and most of its reported hosts are fungi in the genus Sclerotinia [5]. It can parasitize the sclerotia and hypha of Sclerotinia spp. and produce antifungal substances that inhibit host growth [5,[7][8][9][10][11][12][13]. C. minitans can successfully control crop diseases caused by S. sclerotiorum in the field [14][15][16]. C. minitans destroyed the sclerotia, blocked the germination of apothecia by approximately 90% and reduced disease incidence in a bean crop by 50% [5, 17]. As the main effective constituent in commercial biological control agents, C. minitans was first registered in Germany and was used to control the diseases caused by S. sclerotiorum and S. minor in Europe, America, Oceania and Asia. In China, C. min...

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A HOPS protein, CmVps39, is required for vacuolar morphology, autophagy, growth, conidiogenesis and mycoparasitic functions of Coniothyrium minitans

Cited by 18 publications

References 48 publications

A HOPS Protein, MoVps41, Is Crucially Important for Vacuolar Morphogenesis, Vegetative Growth, Reproduction and Virulence in Magnaporthe oryzae

A HOPS Protein, MoVps41, Is Crucially Important for Vacuolar Morphogenesis, Vegetative Growth, Reproduction and Virulence in Magnaporthe oryzae

CmAim24 Is Essential for Mitochondrial Morphology, Conidiogenesis, and Mycoparasitism in Coniothyrium minitans

Mycoparasitism illuminated by genome sequencing and digital gene expression profiling of Coniothyrium minitans, an important biocontrol fungus of the plant pathogen Sclerotinia sclerotiorum

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