A HPTLC densitometric determination of luteolin in Thymus vulgaris and its extracts

Bazylko, Agnieszka; Strzelecka, H

doi:10.1016/j.fitote.2007.01.007

Cited by 18 publications

(7 citation statements)

References 16 publications

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“…Methanol was employed as extracting solvent since it is widely applied for flavonoid extraction [28,35,36]. Electropherograms This finding is in accordance with literature data [28,35] for luteolin concentrations in thyme. However, apigenin concentration varies greatly among different thyme varieties.…”

Section: Application For the Analysis Of Thyme And Parsleysupporting

confidence: 88%

“…However, apigenin concentration varies greatly among different thyme varieties. [35,36] For parsley, the concentration in the crude extract was 154.56 mg % for luteolin and 344.1 mg % for apigenin, while relative to the dry plant, the concentrations were 38.57 mg % and 91.53 mg %, respectively. These results are comparable to literature values for luteolin in parsley.…”

Section: Application For the Analysis Of Thyme And Parsleymentioning

confidence: 97%

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Determination of Luteolin and Apigenin in Herbs by Capillary Electrophoresis with Diode Array Detection

Maher

Alzoman

Al‐Shehri

et al. 2015

Instrumentation Science & Technology

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Capillary electrophoresis with a diode array detector was employed for the determination of luteolin and apigenin in plant extracts. The experimental factors affecting the elution of the analytes were carefully optimized. The final analysis was achieved utilizing a fused silica capillary (58 cm effective length, 75 µm ID) and a background electrolyte solution consisting of borax buffer (20 mM, pH 10.0) and methanol (90: 10, v/v) with a 23 kV driving voltage and detection at 210 nm. The method was fully validated as per the guidelines of the International Conference on Harmonization for Analytical Procedures. The relationship between peak area and concentration was linear between 3 and 800 µg/mL for both compounds with detection limits of 1.05 for luteolin and 0.53 µg/mL for apigenin. The method was employed for the determination of the analytes in extracts of thyme and parsley. Both compounds were resolved from interfering peaks that were present in the extracts. Recovery studies were performed by fortifying the extracts with standards and demonstrated good accuracy with recovery values between 97.29 and Downloaded by [Nanyang Technological University] at 18:34 22 August 2015 2 104.88 % for luteolin and 96.89 and 105.18 % for apigenin. Thyme and parsley were shown to contain high concentrations of luteolin and apigenin.

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Section: Application For the Analysis Of Thyme And Parsleysupporting

confidence: 88%

Section: Application For the Analysis Of Thyme And Parsleymentioning

confidence: 97%

Determination of Luteolin and Apigenin in Herbs by Capillary Electrophoresis with Diode Array Detection

Maher

Alzoman

Al‐Shehri

et al. 2015

Instrumentation Science & Technology

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“…Three phenolic acids (i.e., gallic acid, p -OH-benzoic acid, and rosmarinic acid) and two flavonoids (i.e., luteolin and apigenin) were mainly found in hemp. The most abundant component was luteolin, a flavonoid with antioxidant [ 55 ], anti-inflammatory and antiallergic [ 56 ] activity. Among the few literature studies on the identification of polyphenols in hemp extracts, the presence in hemp essential oil of gallic acid and p -OH benzoic acid has been already reported [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

Chemical Composition and Antioxidant Activity of Thyme, Hemp and Coriander Extracts: A Comparison Study of Maceration, Soxhlet, UAE and RSLDE Techniques

Palmieri

Pellegrini

Ricci

et al. 2020

Foods

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Appropriate and standardized techniques for the extraction of secondary metabolites with interesting biological activity from plants are required. In this work, a comparison of different conventional and unconventional extraction techniques (maceration—M, Soxhlet—S, ultrasound assisted extraction—UAE, and rapid solid-liquid dynamic extraction—RSLDE) was investigated. Bioactive compounds were extracted from Thymus vulgaris L. (thyme), Cannabis sativa L. (industrial hemp) and Coriandrum sativum L. (coriander) and chemically characterized for their volatile fraction and polyphenolic content by means of gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography-ultraviolet (HPLC-UV). Linalool (48.19%, RSLDE) and carvacrol (21.30%, M) for thyme, caryophyllene (54.78%, S) and humulene (14.13%, S) for hemp, and linalool (84.16%, RSLDE) for coriander seeds were the main compounds among terpenes, while thyme was the richest source of polyphenols with rosmarinic acid (51.7 mg/g dry extract-S), apigenin (7.6 mg/g dry extract-S), and luteolin (4.1 mg/g dry extract-UAE) being the most abundant. In order to shed light on their potential as natural food preservatives, the biological activity of the extracts was assessed in terms of antioxidant activity (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid—ABTS˙+, ferric reducing antioxidant power—FRAP, 2,2-diphenyl-1-picrylhydrazyl—DPPH˙ assays) and phenolic content (Folin–Ciocâlteu method). For thyme, Soxhlet extracts showed best performances in FRAP and ABTS˙+ assays (74 mg TE/g dry extract and 134 mg TE/g dry extract, respectively), while Soxhlet and RSLDE extracts recorded similar activity in DPPH˙ (107–109 mg TE/g dry extract). For hemp and coriander, indeed, RSLDE extracts accounted for higher antioxidant activity as evidenced by FRAP (80 mg TE/g dry extract and 18 mg TE/g dry extract, respectively) and ABTS˙+ (557 mg TE/g dry extract and 48 mg TE/g dry extract, respectively) assays. With respect to DPPH˙, the best results were observed for UAE extracts (45 mg TE/g dry extract and 220 mg TE/g dry extract, respectively). Our findings suggest that all the investigated techniques are valid extraction methods to retain bioactive compounds and preserve their activity for application in food and pharmaceutical formulations. Among them, the innovative RSLDE stands out for the slightly higher antioxidant performances of the extracts, coupled with the facility of use and standardization of the extraction process.

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“…apigenin and luteolin was reported to be present in the leaves of Cardiospermum halicacabum [10][11][12] . A UV and polarographic method 13 , RP-HPLC 14 and a HPTLC 15 method was reported for the estimation of luteolin in plant extracts. A rapid RP-HPTLC densitometry method for simultaneous determination of luteolin, apigenin and other major flavonoids in important medicinal plants was reported by Bhandari et al 16 .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Luteolin and Apigenin in Methanolic Leaf Extract of Cardiospermum Halicacabum by HPLC

Rajasekaran¹,

Kalaiselvan²,

Sarathikumar³

et al. 2013

Int. Res. J. Pharm.

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Cardiospermum halicacabum (Sapindaceae) widely spread in Asia, tropical and sub-tropical Africa is commonly consumed as leafy vegetable for the treatment of rheumatoid arthritis in India. The present study was aimed to develop and validate an RP-HPLC separation for the simultaneous estimation of luteolin and apigenin in the leaves of Cardiospermum halicacabum. HPLC separation was accomplished on an oyster ODS 3 column (5 µm, 200 mm × 4.6 mm, i.d.) with gradient elution of potassium dihydrogen phosphate buffer (20 mM, pH 3.5 adjusted with ortho phosphoric acid, v/v) (A) and acetonitrile (B). The elution program as follows; 0-10 min 15-60% B, 10-15 min 60-75%B, 15-20 min 75-100% B, 20-25 min B hold at 100%. The method was validated as per ICH guidelines for system suitability, linearity, specificity, precision, accuracy, LOD and LOQ. The method was linear in the range of 1-70 µg/ml. The relative standard deviation of the method was less than 2% for intra-day and inter-day precision assay and the average recoveries were between 98 and 102%. LOD, LOQ of luteolin and apigenin was found to be 0.03, 0.91 and 0.041, 0.13 µg/ml respectively. The proposed method was found to be linear, specific, accurate, precise and robust

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A HPTLC densitometric determination of luteolin in Thymus vulgaris and its extracts

Cited by 18 publications

References 16 publications

Determination of Luteolin and Apigenin in Herbs by Capillary Electrophoresis with Diode Array Detection

Determination of Luteolin and Apigenin in Herbs by Capillary Electrophoresis with Diode Array Detection

Chemical Composition and Antioxidant Activity of Thyme, Hemp and Coriander Extracts: A Comparison Study of Maceration, Soxhlet, UAE and RSLDE Techniques

Simultaneous Estimation of Luteolin and Apigenin in Methanolic Leaf Extract of Cardiospermum Halicacabum by HPLC

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