A hyaluronidase from Tityus serrulatus scorpion venom: isolation, characterization and inhibition by flavonoids

Pessini, Andréa C.; Takao, Tânia T; Cavalheiro, Elisângela C; Vichnewski, Walter; Sampaio, Suely Vilela; Giglio, José Roberto; Arantes, Eliane Candiani

doi:10.1016/s0041-0101(01)00122-2

Cited by 116 publications

(108 citation statements)

References 32 publications

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“…Immunoblotting showed that only venom components .10 kDa reacted with the antivenom (Rocha-e-Silva et al, 2009b), including hyaluronidase (45 kDa) (Sutti et al, 2014). Studies with T. serrulatus scorpion venom have shown that hyaluronidase has an important role in the action of this venom (Pessini et al, 2001;Horta et al, 2014), and we have also observed the V. dubius enzyme to be edematogenic. However, in contrast to P. nigriventer and T. serrulatus venom-induced edema, the response to V. dubius venom was only partially neutralized by antivenom, perhaps indicating incomplete immunologic identity between the theraphosid venom components responsible for Fig.…”

Section: Discussionsupporting

confidence: 53%

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Silva

Linardi

Antunes

et al. 2015

Journal of Pharmacology and Experimental Therapeutics

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Bites by tarantulas (Theraphosidae, Mygalomorphae) in humans can result in mild clinical manifestations such as local pain, erythema, and edema. Vitalius dubius is a medium-sized, nonaggressive theraphosid found in southeastern Brazil. In this work, we investigated the mediators involved in the plasma extravasation caused by V. dubius venom in rats. The venom caused dose-dependent (0.1-100 mg/site) edema in rat dorsal skin. This edema was significantly inhibited by butyl]benzamide) (SR48968, a neurokinin NK 2 receptor antagonist) had no effect on the venom-induced increase in vascular permeability. In rat hind paws, the venom-induced edema was attenuated by ketoprofen (a nonselective COX inhibitor) administered 15 minutes postvenom. Preincubation of venom with commercial antiarachnid antivenom attenuated the venom-induced edema. These results suggest that the enhanced vascular permeability evoked by V. dubius venom involves serotonin, COX products, neurokinin NK 1 receptors, and nitric oxide formation. The attenuation of hind paw edema by ketoprofen suggests that COX inhibitors could be useful in treating the local inflammatory response to bites by these spiders.

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Section: Discussionsupporting

confidence: 53%

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Silva

Linardi

Antunes

et al. 2015

Journal of Pharmacology and Experimental Therapeutics

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“…The role for this hyaluronidase activity was thought significant only in facilitating tissue penetration and distribution of bioactive venom components in vivo (i.e. as in capture of prey or in human envenomation) (49). In morphological studies, we documented the in vitro and in vivo effects of TSV and some component protein toxins that produce secretory discharge and tissue alterations that are similar to clinical appearances of acute pancreatitis (16,50).…”

Section: Discussionmentioning

confidence: 99%

Vesicle-associated Membrane Protein (VAMP) Cleavage by a New Metalloprotease from the Brazilian Scorpion Tityus serrulatus

Fletcher

Weninger

et al. 2010

Journal of Biological Chemistry

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We present evidence that venom from the Brazilian scorpion Tityus serrulatus and a purified fraction selectively cleave essential SNARE proteins within exocrine pancreatic tissue. Western blotting for vesicle-associated membrane protein type v-SNARE proteins (or synaptobrevins) reveals characteristic alterations to venom-treated excised pancreatic lobules in vitro. Immunocytochemistry by electron microscopy confirms both the SNARE identity as VAMP2 and the proteolysis of VAMP2 as a marked decrease in secondary antibody-conjugated colloidal gold particles that are predominantly associated with mature zymogen granules. Studies with recombinant SNARE proteins were used to determine the specific cleavage site in VAMP2 and the susceptibility of VAMP8 (endobrevin). The VAMP2 cleavage site is between the transmembrane anchor and the SNARE motif that assembles into the ternary SNARE complex. Inclusion of divalent chelating agents (EDTA) with fraction , an otherwise active purified component from venom, eliminates SNARE proteolysis, suggesting the active protein is a metalloprotease. The unique cleavages of VAMP2 and VAMP8 may be linked to pancreatitis that develops following scorpion envenomation as both of these v-SNARE proteins are associated with zymogen granule membranes in pancreatic acinar cells. We have isolated antarease, a metalloprotease from fraction that cleaves VAMP2, and report its amino acid sequence.

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“…HA exists mainly in skin, primarily in dermis in mammalian and is degraded by some different HAases in somatic cells step by step. HAase is found in various organisms: mammalians, bacteria (Streptomyces (Ohya et al 1970), Streptococcus (Hamai et al 1989)), bacteriophage (Baker et al 2002), venom of terrestrial (bees (Kameny et al 1984), hornets (Kolarich et al 2005), scorpions (Pessini et al 2001), snakes (Girish et al 2006), lizards (Tu et al 1983)) and marine (krill (Karlstam et al 1983), lobster (Krishnapillai et al 1999), fishes (Hopkins et al 1998)) animals. HAase is the only acidactive enzyme in the mammalian circulation.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Novel Hyaluronidase Inhibitor From a Marine Actinomycetes Strain

Harunari

Imada

Igarashi

et al. 2015

KLS

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A novel hyaluronidase inhibitor (HI) was isolated from the culture extract of a marine-derived actinomycete strain. This strain MB-PO13 was isolated from ascidian (Molgula manhattensis) in Tokyo Bay. Out of about 1,000 isolates from various marine organisms, strain MB-PO13 had the strongest inhibitory activity and was selected for further study. The strain showed abundant-to-moderate growth on most media, forming a grayish mycelium. On the basis of the taxonomical characteristics, the strain was classified as belonging to the genus of Streptomyces and was named as Streptomyces sp. strain MB-PO13. The structure of HI was elucidated by interpretation of NMR data. HI displayed about 25-fold potent hyaluronidase inhibitory activity against hyaluronidase than glycyrrhizin.

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A hyaluronidase from Tityus serrulatus scorpion venom: isolation, characterization and inhibition by flavonoids

Cited by 116 publications

References 32 publications

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Vesicle-associated Membrane Protein (VAMP) Cleavage by a New Metalloprotease from the Brazilian Scorpion Tityus serrulatus

Isolation and Characterization of a Novel Hyaluronidase Inhibitor From a Marine Actinomycetes Strain

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