A Hybrid Form of Alkaline Phosphatase Produced in FL-Amnion Cells

Abstract

FL-amnion cells have mainly two alkaline phosphatase (AP) isozymes, of which the faster migrating one (FL-AP_F) on 5 % polyacrylamide gel electrophoresis has proved to be identical to the Kasahara isozyme, a tumor-associated AP of intestinal type. But the other slower migrating one (FL-AP_s) remains to be characterized. Immunological and enzymic examination of purified FL-AP_S revealed that it is a hybrid form of AP consisting of one subunit with a molecular weight of 66,200 coming from the same subunit as that of FL-AP_F and another one with a molecular weight of 58,000 from placental AP subunit with modified glycosylation.