Tianjin Port is one of the largest ports in northern China. Liquefied natural gas (LNG) ships are one of the most special ship types, and their navigation safety and efficiency has become the top concern of the port authority. There are two LNG berths at the port, and the annual arrivals, which reach more than 100, increasingly influence other ships. The objective of this study is to evaluate the influence of LNG ships on other ships in a quantitative way. To realize this, a simulation system is established by analyzing the factors affecting waterway transit efficiency. The software Arena is adopted to simulate the arrival and departure of the ships at Tianjin Port and to simulate how the average waiting time and the average queue length in the port area are affected by the LNG ships. A traffic system for the two ship types is formulated, and the mutual influences between them are expressed by the inbound and outbound waterway states. The simulations are performed under both existing and new ship traffic regulations. Cases in which the number of LNG ships gradually increases are simulated comprehensively. The simulation model as well as the results can serve as a good reference for the local port authority in the formulation of traffic regulations.