A hydrofluoric acid-free method to dissolve and quantify silica nanoparticles in aqueous and solid matrices

Bossert, David; Urban, Dominic A.; Maceroni, Mattia; Ackermann-Hirschi, Liliane; Haeni, Laetitia; Yajan, Phattadon; Spuch‐Calvar, Miguel; Rothen‐Rutishauser, Barbara; Rodríguez‐Lorenzo, Laura; Petri‐Fink, Alke; Schwab, Fabienne

doi:10.1038/s41598-019-44128-z

Cited by 32 publications

(26 citation statements)

References 46 publications

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“…SiO 2 NPs and subcellular distribution within the leaf. The SiO 2 NPs were synthesized and characterized according to a previously established procedure 31,32 adapted from an earlier work 56 . Briefly, one equivalent of tetraethyl orthosilicate (10 ml, >99%; Sigma-Aldrich) was added to an equilibrated reaction mixture at 70 °C containing two equivalents of ultrapure water (Milli-Q, 18.2 MΩ arium 611 DI, Sartorius Stedim Biotech), and absolute ethanol (81 ml) as a solvent under basic conditions (2.93 ml of 25% NH 3 ).…”

Section: Methodsmentioning

confidence: 99%

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Silica nanoparticles enhance disease resistance in Arabidopsis plants

et al. 2020

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Section: Methodsmentioning

confidence: 99%

“…Inductively coupled plasma-optical emission spectroscopy and gravimetry served to quantify the SiO 2 concentration (methods described in ref. 31 ).…”

Section: Methodsmentioning

confidence: 99%

Silica nanoparticles enhance disease resistance in Arabidopsis plants

et al. 2020

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“…Moreover, the conventional solubilisation of silica particles in organic matter with hydrofluoric acid (HF) is subject to critical handling guidelines and requires specialized labs and personnel [ 38 ]. Recently, Bossert et al (2019) proposed a HF-free hot alkaline lysis followed by acid treatment to dissolve silica particles; silicon was then detected by ICP-OES or by a colorimetric method with a limit of detection (LOD) being in the range of 40–100 mg/L [ 39 ]. Of note, cells exposed in vitro to approximately 10 µg SAS per mL likely underscore this LOD by at least one order of magnitude, unless the cell number is scaled up to very high amounts.…”

Section: Introductionmentioning

confidence: 99%

Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner

Wiemann¹,

Vennemann²,

Venzago

et al. 2021

Nanomaterials

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Various cell types are compromised by synthetic amorphous silica (SAS) if they are exposed to SAS under protein-free conditions in vitro. Addition of serum protein can mitigate most SAS effects, but it is not clear whether this is solely caused by protein corona formation and/or altered particle uptake. Because sensitive and reliable mass spectrometric measurements of SiO2 NP are cumbersome, quantitative uptake studies of SAS at the cellular level are largely missing. In this study, we combined the comparison of SAS effects on alveolar macrophages in the presence and absence of foetal calf serum with mass spectrometric measurement of 28Si in alkaline cell lysates. Effects on the release of lactate dehydrogenase, glucuronidase, TNFα and H2O2 of precipitated (SIPERNAT® 50, SIPERNAT® 160) and fumed SAS (AEROSIL® OX50, AEROSIL® 380 F) were lowered close to control level by foetal calf serum (FCS) added to the medium. Using a quantitative high resolution ICP-MS measurement combined with electron microscopy, we found that FCS reduced the uptake of particle mass by 9.9% (SIPERNAT® 50) up to 83.8% (AEROSIL® OX50). Additionally, larger particle agglomerates were less frequent in cells in the presence of FCS. Plotting values for lactate dehydrogenase (LDH), glucuronidase (GLU) or tumour necrosis factor alpha (TNFα) against the mean cellular dose showed the reduction of bioactivity with a particle sedimentation bias. As a whole, the mitigating effects of FCS on precipitated and fumed SAS on alveolar macrophages are caused by a reduction of bioactivity and by a lowered internalization, and both effects occur in a particle specific manner. The method to quantify nanosized SiO2 in cells is a valuable tool for future in vitro studies.

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“…Redness, swelling, heat, and pain appear after initial HF contact through the skin. Cloudy and creamy blisters may gradually occur, with ulcers and tissue necrosis developing in the final stage [2,3]. HF burns are currently managed by removing HF, preventing systemic toxicity, applying supportive treatment, and managing local infection [4].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-based hydrogels to treat hydrofluoric acid burns and prevent infection

Huang

Yeh

et al. 2021

Drug Deliv. and Transl. Res.

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There is an urgent need for treatments for hydrofluoric acid (HF) burns and their derivative problems that prevent hydrogen ion dissociation and fluoride ion binding to tissues. This study evaluated the ability of chitosan-based hydrogels combined with a buffer solution containing either boric acid or Tris and calcium gluconate (CHS-BA-CG and CHS-Tris-CG) to repair HF burn wounds and prevent wound infections. We assessed calcium release rates and biocompatability and constructed a mouse HF burn model to assess the tissue repair effects of the hydrogels. Finally, we performed disc diffusion tests from burn tissue and quantified the bacterial counts to assess the anti-infection properties of the hydrogels. Calcium was gradually released in the CHS-BA-CG and CHS-Tris-CG groups (73% and 43%, respectively, after 48 h). The cell viabilities at 48 h after HF burn in these groups were significantly higher than those in the phosphate-buffered saline (PBS) and CG-treated groups. Histopathological evaluation showed a clear boundary between the epidermal and dermal layers in both CHS-BA-CG and CHS-Tris-CG-treated groups, indicating their effectiveness in tissue repair. In the disc diffusion test, CHS-BA-CG and CHS-Tris-CG exhibited larger inhibition zones against Acinetobacter baumannii than those for PBS and CG. The bacterial counts on HF burn wounds were significantly lower in the CHS-BA-CG and CHS-Tris-CG-treated groups than those in the PBS and CG-treated groups. The in vitro studies demonstrated the biocompatibility and antimicrobial effects of the CHS-BA-CG and CHS-Tris-CG hydrogels. Both gels also demonstrated tissue repair and anti-infection effects. Thus, chitosan-based hydrogels may be candidates for HF burn therapy.

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A hydrofluoric acid-free method to dissolve and quantify silica nanoparticles in aqueous and solid matrices

Cited by 32 publications

References 46 publications

Silica nanoparticles enhance disease resistance in Arabidopsis plants

Silica nanoparticles enhance disease resistance in Arabidopsis plants

Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner

Chitosan-based hydrogels to treat hydrofluoric acid burns and prevent infection

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