Observed runoff is typically the only variable applied to calibrate ecohydrological models, which can simulate runoff well but cannot guarantee reliable reproduction of other hydrological components (e.g., evapotranspiration (ET) and total terrestrial water storage change (TWSC)). This study explores the potential of estimating hydrological components (runoff, ET, TWSC, groundwater, and root‐zone water storage.) using a conceptual model driven and calibrated by multisource remote sensing data only. The performances of the model were evaluated in 13 catchments with different geological, climatic and vegetation conditions. Results show that the model was encouraging in simulating monthly runoff (median KGE, 0.71; RMSE, 19.4 mm/mon), ET (median KGE, 0.86; RMSE, 9.0 mm/mon), and TWSC (median KGE, 0.58; RMSE, 26.6 mm/mon). The good positive correlation between root‐zone water storage and gross primary productivity also indicates the effectiveness of the model in simulating root‐zone water storage. This study offers new prospects for estimating key ecohydrological components precisely, especially in catchments that are difficult to monitor (e.g., karst regions) and catchments with limited observation data (e.g., ungauged regions).