A hydrothermal reaction of an aqueous solution of BSA yields highly fluorescent N doped C-dots used for imaging of live mammalian cells

Kumar, Vijay Bhooshan; Sheinberger, Jonathan; Porat, Ze’ev; Shav‐Tal, Yaron; Gedanken, Aharon

doi:10.1039/c6tb00519e

Cited by 47 publications

(67 citation statements)

References 35 publications

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“…Moreover, the stabilization of MoS 2 nanosheets by Tris is not destroyed at high temperature (e.g., 220 °C). As previously reported, protein BSA will be decomposed at high temperature . As indicated in Figure , the absorption peak from BSA disappeared to indicate the complete decomposition of BSA under hydrothermal treatment (refer to pink and blue lines).…”

Section: Resultssupporting

confidence: 67%

Tris‐Stabilized MoS₂ Nanosheets with Robust Dispersibility and Facile Surface Functionalization

Guan

You

Liu

et al. 2019

Adv Materials Inter

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Here, tris(hydroxymethyl)aminomethane (i.e., Tris) is reported to be an effective protecting agent to stabilize 2D nanomaterials in water for various enhanced applications. As demonstrated, molybdenum sulfide (MoS2) nanosheets are first exfoliated from MoS2 bulk under a low‐energy sonication in the assistance of protein bovine serum albumin (BSA), and then BSA molecules are replaced with Tris to stabilize the exfoliated MoS2 nanosheets. The Tris‐stabilized MoS2 nanosheets have a robust dispersibility to resist long‐term storage (e.g., 2.5 years), high temperature (e.g., 220 °C), and high speed centrifugation (e.g., 12 000 rpm). Furthermore, they also exhibit high biocompatibility and promising photothermal effect, which provide a better performance in photothermal therapy for inhibiting the growth of tumor. In addition, the surface Tris molecules readily introduce the grafting of polydopamine on MoS2 nanosheets to form a universal platform for coating of other functional polymers on nanosheets. Overall, this work not only brings more opportunities to exploit the outstanding applications of 2D nanomaterials, but also suggests a new and effective route to prepare the composite of nanosheets and functional polymers for their synergistic properties.

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Section: Resultssupporting

confidence: 67%

Tris‐Stabilized MoS₂ Nanosheets with Robust Dispersibility and Facile Surface Functionalization

Guan

You

Liu

et al. 2019

Adv Materials Inter

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“…Besides, using quinine sulfate in 0.1 m sulfuric acid solution (quantum yield 54%) as the standard, the fluorescence quantum yield of the CNDs was calculated to be 6% (seeing the data of quantum yield in Table S1, Supporting Information), higher than that of the reported Gd‐CNDs (5.4%), which was due to their surface chemistry, such as “defect sites” located on the surface of resultant CNDs. The strong green fluorescence emission of CNDs could be attributed to modulation of the chemical and electronic characteristics of CNDs, which was induced by the introduction of P atoms . These results indicated that the solvothermal method is a promising approach to synthesize the fluorescence CNDs without using any catalyst.…”

Section: Resultsmentioning

confidence: 89%

Stable Fluorescence of Green‐Emitting Carbon Nanodots as a Potential Nanothermometer in Biological Media

Jiang

et al. 2016

Part & Part Syst Charact

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1 of 7) 1600197Temperature measurement in biology and medical diagnostics is of great importance. Herein, a novel carbon nanodot (CND) based fluorescent nanothermometry device for spatially resolved temperature measurements is demonstrated. The fluorescence CNDs are prepared by a simple one-pot solvothermal method using sucrose as carbon source. Resultant CNDs show stable green fluorescence at 520 nm with high quantum yield (≈6%). The fluorescence of resultant CNDs exhibits a reversible linear response to temperature in a wide range of 20-85 °C. Moreover, the temperature resolution better than 0.5 °C and high sensitive variation of ≈1.3% °C −1 are observed in a broad physiological temperature range of 20-40 °C. Therefore, the as-prepared CNDs possess high water solubility, stable fluorescence, small size, and good biocompatibility, which make them promising candidate for thermometry and cell imaging in biological media. stability, serious toxicity, and high cost, have limited their further application.The carbon nanodots (CNDs), a new family member of carbon nanomaterials, are generally composed of sp 2 hybridized carbon atoms with abundant oxygenous and hydrogenous residues with sizes below 10 nm. [10,11] Compared to the conventional fluorescent nanomaterials (SQDs, metal nanoclusters, and organic fluorescent dyes), CNDs possess numerous superior features including easy preparation and functionalization, no/low toxicity, and high photostability, thus demonstrating a variety of potential applications, such as sensing, biomedicine, catalysis, and optoelectronic devices. [12][13][14][15] To date, many efforts have been done to study the preparations, properties, and applications of CNDs, but most of them show intense emission only in the blue-light region, and the long-wavelength (i.e., green-, yellow-, to red-light) emissions are usually very weak. [16][17][18] Such a drawback restricts further applications of CNDs, particularly in the biology-relevant fields because of the common blue autofluorescence of biological matrix and photodamage of biological tissues by ultraviolet excitation light. Furthermore, many CNDs-based nanosensors have been designed and played an important role in bio-analysts in vitro and vivo. [19][20][21][22] For example, Zhu et al. reported a facile and high output strategy for the fabrication of highly photoluminescent CNDs which could be utilized as a biosensor reagent for the detection of Fe 3+ in bio-systems; [19] Zhang group reported an effective fluorescent probe for sensing mercuric and iodide ions based on the nitrogen-doped CNDs; [20] Nie et al. applied the CNDs as an internal reference to construct a ratiometric pH sensor in living cells, [21] while few CNDs-based thermometers are reported. [23][24][25][26] More recently, our group used a hydrothermal route to prepare water-stable luminescent CNDs as nanosensors for the detection of pH and temperature; [23] then we also designed a "turn-on" chemosensor for detecting temperature through surface-modified CNDs and the carbon-dot-based...

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“…The peak in the C1s spectrum at the binding energy of 284.3 eV indicates the existence of a C=C structure in the prepared CDs, and the peaks at 285.7 and 287.1 eV correspond to C–N/C–O and C=O, respectively (Figure B) . The N1s spectrum of the CDs deconvolutes to two peaks at 399.6 and 401.6 eV (Figure C), which can be attributed to (C) 3 ‐N and N–H groups, respectively . The O1s spectrum exhibits two primary bands at 530.7 and 532.2 eV, attributed to ‐OH and C–O (Figure D) .…”

Section: Resultsmentioning

confidence: 96%

One‐Pot Synthesis of Highly Fluorescent Carbon Dots from Spinach and Multipurpose Applications

et al. 2018

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Highly fluorescent carbon dots (CDs) have been prepared from natural spinach by a hydrothermal method. In this work, we have demonstrated that the CDs have a high quantum yield of 53 %, and exhibit special responses to 2-nitrophenol (2-NP) and 4-nitrophenol (4-NP). Solutions of the CDs showed clear fluorescence quenching in the presence of 2-NP and 4-NP; the detection limits of 2-NP and 4-NP were 1.06 and 0.50 μM,

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A hydrothermal reaction of an aqueous solution of BSA yields highly fluorescent N doped C-dots used for imaging of live mammalian cells

Cited by 47 publications

References 35 publications

Tris‐Stabilized MoS₂ Nanosheets with Robust Dispersibility and Facile Surface Functionalization

Tris‐Stabilized MoS₂ Nanosheets with Robust Dispersibility and Facile Surface Functionalization

Stable Fluorescence of Green‐Emitting Carbon Nanodots as a Potential Nanothermometer in Biological Media

One‐Pot Synthesis of Highly Fluorescent Carbon Dots from Spinach and Multipurpose Applications

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A hydrothermal reaction of an aqueous solution of BSA yields highly fluorescent N doped C-dots used for imaging of live mammalian cells

Cited by 47 publications

References 35 publications

Tris‐Stabilized MoS2 Nanosheets with Robust Dispersibility and Facile Surface Functionalization

Tris‐Stabilized MoS2 Nanosheets with Robust Dispersibility and Facile Surface Functionalization

Stable Fluorescence of Green‐Emitting Carbon Nanodots as a Potential Nanothermometer in Biological Media

One‐Pot Synthesis of Highly Fluorescent Carbon Dots from Spinach and Multipurpose Applications

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Tris‐Stabilized MoS₂ Nanosheets with Robust Dispersibility and Facile Surface Functionalization

Tris‐Stabilized MoS₂ Nanosheets with Robust Dispersibility and Facile Surface Functionalization