A Hylarana latouchii Skin Secretion-Derived Novel Bombesin-Related Pentadecapeptide (Ranatensin-HLa) Evoke Myotropic Effects on the in vitro Rat Smooth Muscles

Abstract

Amphibians have developed successful defensive strategies for combating predators and invasive microorganisms encountered in their broad range of environments, which involve secretion of complex cocktails of noxious, toxic and diverse bioactive molecules from the skins. In recent years, amphibian skin secretions have been considered as an extraordinary warehouse for the discovery of therapeutic medicines. In this study, through bioactivity screening of the Hylarana latouchii skin secretion-derived fractions, a novel peptide belonging to ranatensin subfamily (ranatensin-HLa) was discovered, and structurally and pharmacologically-characterised. It consists of 15 amino acid residues, pGlu-NGDRAPQWAVGHFM-NH2, and its synthetic replicate was found to exhibit pharmacological activities on increasing the contraction of the in vitro rat bladder and uterus smooth muscles. Corresponding characteristic sigmoidal dose-response curves with EC50 values of 7.1 nM and 5.5 nM were produced, respectively, in bladder and uterus. Moreover, the precursor of ranatensin-HLa showed a high degree of similarity to those of bombesin-like peptides from Odorrana grahami and Odorrana schmackeri. Hylarana latouchii skin continues to serve as a storehouse with diverse lead compounds for the development of therapeutically effective medicines.

Modification and Targeted Design of N-Terminal Truncates Derived from Brevinin with Improved Therapeutic Efficacy

“…After the authentic and unequivocal sequence was obtained, peptides (B1A and its analogues) were synthesised via the Solid Phase Peptide Synthesis (SPPS) as described previously [ 17 ]. Briefly, the protected group of each amino acid was removed by piperidine/N, N-dimethylformamide (DMF) (20/80, V / V ).…”

Exploring the active core of a novel antimicrobial peptide, palustrin-2LTb, from the Kuatun frog, Hylarana latouchii, using a bioinformatics-directed approach

“…In tropical and subtropical Asia only, there have been around 40 different Hylarana amphibian species reported to date [17] . Like in other anurans, researchers have found many AMPs in the skin secretions of Hylarana latouchii, some of which show great research value [18] .…”

Identification of a new myotropic decapeptide from the skin secretion of the red-eyed leaf frog, Agalychnis callidryas

“…Following the first isolated BK, more than 70 BK-like peptides have further been discovered [2]. Many BK and BK-related peptides, which have identified from anuran species, were isolated from the family Bombinatatoridae, Hylidae, and Ranidae [3][4][5]. Despite diverse primary sequences, numerous typical BKs are with relatively conservative domains, with the N-terminus/C-terminus of conserved PPGF motif, Arg 1 , and Ser 6 frequently substitution, the deletion of Arg around N-terminus/C-terminus [6].…”