A hyper-diverse genus of acanthocephalans revealed by tree-based and non-tree-based species delimitation methods: Ten cryptic species of Neoechinorhynchus in Middle American freshwater fishes

Pinacho-Pinacho, Carlos Daniel; García-Varela, Martìn; Sereno-Uribe, Ana L.; León, Gerardo Pérez‐Ponce de

doi:10.1016/j.ympev.2018.05.023

Cited by 49 publications

(44 citation statements)

References 92 publications

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“…Coupled with climatic oscillations and corresponding sea-level changes during the Pleistocene, topographically complex features likely created refugia or geographic dispersal barriers for terrestrial animals, triggering allopatric local speciation or genetic differentiation of isolated populations (Avise 2000;Song et al 2009;Huang et al 2010;Liu et al 2012;Yan et al 2013). Although the dynamic effects of these variables have been studied in many North American and European species (Paupério et al 2012;Malaney et al 2013;Říčanová et al 2013;Song et al 2014;Satler et al 2017;Pinacho-Pinacho et al 2018), species in subtropical and tropical zones have been comparatively neglected. In southern China, both climate and topographic complexity may have contributed to the phylogeographic structure of endemic species and the formation of within-species genetic lineages (Liu et al 2012;Tsai et al 2014;Feng et al 2017), and recent molecular taxonomic studies have already revealed previously undetected geographic lineages or even cryptic species (Jing et al 2007;Liu et al 2012;He et al 2013;Huang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeographic analyses and species delimitations reveal that Leopoldamys edwardsi (Rodentia: Muridae) is a species complex

Kong

Wang

et al. 2019

Integrative Zoology

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Leopoldamys edwardsi is a species with wide distribution ranges in southern China but is not discussed in studies on geographic variation and species differentiation. We used 2 mitochondrial (Cytb, CO1) and 3 nuclear (GHR, IRBP and RAG1) genes to clarify species phylogeography and geographical differentiation. Maximum likelihood (ML) and Bayesian phylogenetic inference (BI) trees consistently indicated that L. edwardsi is a species complex containing 3 main lineages with high Kimura‐2‐parameter (K2P) divergences (i.e. lineages LN, LS and LHN) found in the northern and southern China and Hainan Island, respectively. The 3 species delimitation methods, automated barcoding gap discovery, Bayesian poisson tree process analysis and Bayesian phylogenetics and phylogeography, consistently supported the existence of cryptic species. Divergence times among the main lineages were inferred to be during the Pleistocene, with LHN/LS split at 1.33 Ma and LN/(LHN+LS) at 2.61 Ma; the diversifications of L. edwardsi complex might be caused by the rapid uplifts of Tibetan Plateau, paleoclimate change and complex topography. The divergence between LHN and LS was probably related to the separation of Hainan Island from the mainland via the formation of the Qiongzhou Strait. Lineages LN and (LS+LHN) likely diverged due to the Wuyi‐Nanling mountain range forming a dispersal barrier. Our results suggested that L. edwardsi complex contains at least 3 distinct species: LHN represents L. hainanensis, endemic to Hainan Island and previously considered as a subspecies L. e. hainanensis; LS represents a cryptic species distributed throughout the southern Chinese continent; and LN represents the nominotypical species L. edwardsi.

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Section: Introductionmentioning

confidence: 99%

Molecular phylogeographic analyses and species delimitations reveal that Leopoldamys edwardsi (Rodentia: Muridae) is a species complex

Kong

Wang

et al. 2019

Integrative Zoology

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“…Due to the lack of sufficient sequences of cox1 sequences of N. agilis complex in GenBank for comparison, we did not perform molecular analysis of these specimens with this gene. Pinacho-Pinacho et al showed that the cox1 gene is an informative molecular marker compared to the ITS and 28S rDNA genes for detection of cryptic species complexes in Neoechinorhynchus in Middle America [25]. However, their analysis is for the species inhabiting fresh and brackish water fish across Middle America, a different zone from our study areas.…”

Section: Molecular Analysismentioning

confidence: 61%

“…All hooks serrated longitudinally (Figs. [13][14][15][16][23][24][25], rooted, most robust with longest roots anteriorly ( Fig. 14), smallest posteriorly, equal in length in each circle.…”

Section: Redescriptionmentioning

confidence: 99%

“…It has been found in 6 species in 4 families of marine fish in the Persian Gulf and in the Pacific Ocean off Vietnam. In species of Neoechinorhynchus, high level of variation in the cox1 gene would provide better resolution of the relationships within closely related taxa [25]. Due to the lack of sufficient sequences of cox1 sequences of N. agilis complex in GenBank for comparison, we did not perform molecular analysis of these specimens with this gene.…”

Section: Molecular Analysismentioning

confidence: 99%

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On theNeoechinorhynchus agilis(Acanthocephala: Neoechinorhynchidae) complex, with a description ofNeoechinorhynchus ponticusn. sp. fromChelon auratusin the Black Sea

Amin¹,

Sharifdini

Heckmann

et al. 2020

Parasite

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We recognize four species in the Neoechinorhynchus agilis complex. We studied specimens of Neoechinorhynchus (Hebesoma) personatus Tkach, Sarabeev & Shvetsova, 2014 from Mugil cephalus in the Mediterranean Sea off Tunisia and in the Black Sea, and also specimens of Neoechinorhynchus ponticus n. sp. from Chelon auratus Risso in the Black Sea. Specimens from M. cephalus at both locations were similar. All structures of N. ponticus n. sp. were considerably smaller than those of N. personatus. Two other species of the N. agilis complex are recognized: Neoechinorhynchus agilis (Rudolphi, 1819) sensu stricto from various hosts in the Atlantic and the Mediterranean, and Neoechinorhynchus yamagutii Tkach, Sarabeev & Shvetsova, 2014 from M. cephalus and Planiliza haematocheila in the Pacific, especially the Sea of Japan. Neoechinorhynchus dimorphospinus Amin & Sey, 1996 from marine fish in the Persian Gulf and the Pacific Ocean off Vietnam may be a candidate for membership in the N. agilis complex. X-ray scans of gallium cut and intact hooks of N. personatus and N. ponticus showed differences in the mineral content of hooks with higher sulfur levels in smaller hooks and in hooks from specimens in the Black Sea compared to specimens from the Mediterranean. The relatively high genetic differences between N. ponticus n. sp. and other species of Neoechinorhynchus using a partial 18S rDNA dataset support its independent status. Neoechinorhynchus ponticus n. sp. and N. personatus have a common ancestor with species of Neoechinorhynchus collected from saltwater fish.

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“…However, defining a new species when relying solely on molecular information is inadequate. Empirical studies have shown that using molecular methods alone sometimes over-split populations that might not represent distinct species, leading to taxonomic inflation (Bauer et al, 2011;Pinacho-Pinacho, García-Varela, Sereno-Uribe, & Pérez-Ponce, 2018;Villamil et al, 2019;Zhang, Kapli, Pavlidis, & Stamatakis, 2013). The uncertainties in delimiting species boundaries based on the molecular data ideally need to be tested and carefully verified using other sources of information (e.g.…”

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confidence: 99%

Integrative taxonomy uncovers hidden species diversity in the rheophilic genus Potamometra (Hemiptera: Gerridae)

Zheng

Zhu

et al. 2019

Zoologica Scripta

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The genus Potamometra Bianchi, 1896 represents big rheophilic semi‐aquatic bugs that typically inhabit middle‐altitude mountainous streams. Here, we integrated molecular and morphological data for delimiting species boundaries and understanding the evolutionary history of the genus Potamometra. Twenty‐seven complete mitochondrial genomes of Potamometra were sequenced, with samples representing most of the known geographically distributed locations around the Sichuan Basin. The results of different species delimitation methods (ABGD, bPTP, GMYC and BPP) based on the monolocus or multilocus data strongly supported the existence of two cryptic new species (Potamometra anderseni Zheng, Ye & Bu, sp. nov. and Potamometra zhengi Zheng, Ye & Bu, sp. nov.) although more entities were found in the tree‐based delimitation methods. The two new species were successfully validated using morphological characters within a detailed taxonomic framework. Phylogenetic analyses supported the reciprocal monophyly of the seven highly node‐supported clades, which were matched with the five known species and two new taxa. A novel gene arrangement pattern that two trnF (trnF1 and trnF2) genes separated by an intergenic spacer (IGS) were found in all the species except the sister group of Potamometra berezowskii Bianchi, 1896 and Potamometra linnavuorii Chen, Nieser & Bu, 2016. This gene rearrangement event could be explained by the tandem duplication and random loss (TDRL) model. Our study emphasized that the combination of molecular sequence data, morphological characters and mitochondrial structural information could improve the accuracy of species delimitation.

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A hyper-diverse genus of acanthocephalans revealed by tree-based and non-tree-based species delimitation methods: Ten cryptic species of Neoechinorhynchus in Middle American freshwater fishes

Cited by 49 publications

References 92 publications

Molecular phylogeographic analyses and species delimitations reveal that Leopoldamys edwardsi (Rodentia: Muridae) is a species complex

Molecular phylogeographic analyses and species delimitations reveal that Leopoldamys edwardsi (Rodentia: Muridae) is a species complex

On theNeoechinorhynchus agilis(Acanthocephala: Neoechinorhynchidae) complex, with a description ofNeoechinorhynchus ponticusn. sp. fromChelon auratusin the Black Sea

Integrative taxonomy uncovers hidden species diversity in the rheophilic genus Potamometra (Hemiptera: Gerridae)

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