2022
DOI: 10.1101/2022.12.18.520953
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AGFPsplicing reporter in acoilinmutant background reveals links between alternative splicing, siRNAs and coilin function inArabidopsis thaliana

Abstract: Coilin is a scaffold protein essential for the structure of Cajal bodies, which are nucleolar-associated, nonmembranous organelles that coordinate the assembly of nuclear ribonucleoproteins including spliceosomal snRNPs. To learn more about coilin functions and pathways in plants, we conducted a genetic suppressor screen using a coilin mutant in Arabidopsis thaliana and performed an immunoprecipitation-mass spectrometry analysis on coilin protein. The coi1 mutations modify alternative splicing of a GFP reporte… Show more

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