2023
DOI: 10.1002/biot.202300064
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A Rehmannia glutinosa caffeic acid O‐methyltransferase functional identification: Reconstitution of the ferulic acid biosynthetic pathway in Saccharomyces cerevisiae using Rehmannia glutinosa enzymes

Abstract: Rehmannia glutinosa produces many pharmacological natural components, including ferulic acid (FA) which is also an important precursor of some medicinal ingredients, so it is very significant to explore FA biosynthesis for enhancing the production of FA and its derivations. This study aimed to determine and reconstitute the R. glutinosa FA biosynthetic pathway from phenylalanine (Phe) metabolism in Saccharomyces cerevisiae as a safe host for the biosynthesis of plant‐derived products. Although plant caffeic ac… Show more

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Cited by 2 publications
(1 citation statement)
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“…Consequently, we chose the same enzyme for the construction of the curcumin artificial biosynthetic pathway. Meanwhile, other COMT enzymes were expressed in yeast including the ones from Nicotiana tabacum and Rehmannia glutinosa. , Supplementation of 30 mg/L caffeic acid to JI2 strain resulted in the production of 497.6 ± 35.7 μg/L of curcumin. The absence of any other curcuminoids indicates that the Pp FerA, Cl DCS, and Cl CURS1 enzymes cannot utilize caffeic acid to produce alternative curcuminoid compounds.…”
Section: Resultsmentioning
confidence: 99%
“…Consequently, we chose the same enzyme for the construction of the curcumin artificial biosynthetic pathway. Meanwhile, other COMT enzymes were expressed in yeast including the ones from Nicotiana tabacum and Rehmannia glutinosa. , Supplementation of 30 mg/L caffeic acid to JI2 strain resulted in the production of 497.6 ± 35.7 μg/L of curcumin. The absence of any other curcuminoids indicates that the Pp FerA, Cl DCS, and Cl CURS1 enzymes cannot utilize caffeic acid to produce alternative curcuminoid compounds.…”
Section: Resultsmentioning
confidence: 99%