A
            <i>Rhodococcus qsdA</i>
            -Encoded Enzyme Defines a Novel Class of Large-Spectrum Quorum-Quenching Lactonases

Uroz, Stéphane; Oger, Philippe; Chapelle, Emilie; Adeline, Marie-Thérèse; Faure, Denis; Dessaux, Yves

doi:10.1128/aem.02014-07

Cited by 176 publications

(188 citation statements)

References 52 publications

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“…(56) and was subsequently found in the genomes of countless bacteria and eukaryotes (26,54,133,145,153,170,203,213,216). AHL lactonases belong to either the metallo-␤-lactamase family of proteins (108,121,190) or the phosphotriesterase-like family (195). Lactonolysis of the AHL ring leads to an inactive product that no longer activates the AHL QS system, with consequent attenuation of virulence, as observed in various infection models (31,55,149,188).…”

Section: Targeting Bacterial Signalingmentioning

confidence: 99%

The Multiple Signaling Systems Regulating Virulence in Pseudomonas aeruginosa

Nadal‐Jimenez

Koch

Thompson

et al. 2012

Microbiol Mol Biol Rev

641

626

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SUMMARY Cell-to-cell communication is a major process that allows bacteria to sense and coordinately react to the fluctuating conditions of the surrounding environment. In several pathogens, this process triggers the production of virulence factors and/or a switch in bacterial lifestyle that is a major determining factor in the outcome and severity of the infection. Understanding how bacteria control these signaling systems is crucial to the development of novel antimicrobial agents capable of reducing virulence while allowing the immune system of the host to clear bacterial infection, an approach likely to reduce the selective pressures for development of resistance. We provide here an up-to-date overview of the molecular basis and physiological implications of cell-to-cell signaling systems in Gram-negative bacteria, focusing on the well-studied bacterium Pseudomonas aeruginosa . All of the known cell-to-cell signaling systems in this bacterium are described, from the most-studied systems, i.e., N -acyl homoserine lactones (AHLs), the 4-quinolones, the global activator of antibiotic and cyanide synthesis (GAC), the cyclic di-GMP (c-di-GMP) and cyclic AMP (cAMP) systems, and the alarmones guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), to less-well-studied signaling molecules, including diketopiperazines, fatty acids (diffusible signal factor [DSF]-like factors), pyoverdine, and pyocyanin. This overview clearly illustrates that bacterial communication is far more complex than initially thought and delivers a clear distinction between signals that are quorum sensing dependent and those relying on alternative factors for their production.

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Section: Targeting Bacterial Signalingmentioning

confidence: 99%

The Multiple Signaling Systems Regulating Virulence in Pseudomonas aeruginosa

Nadal‐Jimenez

Koch

Thompson

et al. 2012

Microbiol Mol Biol Rev

641

626

View full text Add to dashboard Cite

show abstract

“…The most studied AHL-lactonases belong to 2 families: Zn-dependent metallo-b-lactamases (eg the autoinducer inactivating enzyme (AiiA) of Bacillus sp. as a prototypical member (Dong et al 2000)) and metallo-dependent phosphotriesterases (Uroz et al 2008). Since the isolation of the first AHL-degrading enzyme, AiiA and the cloning of the gene aiiA encoding it from Bacillus sp.…”

Section: Enzymes That Degrade Biofilm-forming Qs Signalsmentioning

confidence: 99%

Mini-review: Inhibition of biofouling by marine microorganisms

2013

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Any natural or artificial substratum exposed to seawater is quickly fouled by marine microorganisms and later by macrofouling species. Microfouling organisms on the surface of a substratum form heterogenic biofilms, which are composed of multiple species of heterotrophic bacteria, cyanobacteria, diatoms, protozoa and fungi. Biofilms on artificial structures create serious problems for industries worldwide, with effects including an increase in drag force and metal corrosion as well as a reduction in heat transfer efficiency. Additionally, microorganisms produce chemical compounds that may induce or inhibit settlement and growth of other fouling organisms. Since the last review by the first author on inhibition of biofouling by marine microbes in 2006, significant progress has been made in the field. Several antimicrobial, antialgal and antilarval compounds have been isolated from heterotrophic marine bacteria, cyanobacteria and fungi. Some of these compounds have multiple bioactivities. Microorganisms are able to disrupt biofilms by inhibition of bacterial signalling and production of enzymes that degrade bacterial signals and polymers. Epibiotic microorganisms associated with marine algae and invertebrates have a high antifouling (AF) potential, which can be used to solve biofouling problems in industry. However, more information about the production of AF compounds by marine microorganisms in situ and their mechanisms of action needs to be obtained. This review focuses on the AF activity of marine heterotrophic bacteria, cyanobacteria and fungi and covers publications from 2006 up to the end of 2012.

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“…An AHL-degrading enzyme was detected from B. thuringiensis that affects the virulence of the gram-negative bacterium Erwinia carotovora in the root system of pepper (Park et al, 2008). So far, 2 types of enzymes that inactivate AHLs have been identified in several species/genera of bacteria: the AHL lactonases that cause lactonolysis (opening of the gamma-butyrolactone ring) resulting in acyl-homoserine with reduced biological activity, and the AHL acylases that break the amide linkage of AHLs to produce homoserine lactone and fatty acids with no biological activity (Uroz et al, 2008). Abolishment of the production of the quorumsensing signals, known as quorum quenching (QQ), results in significantly defective biofilm formation, and thus reduces the ability of the pathogen to colonize the host, resulting in biocontrol.…”

Section: Concepts Of Quorum Sensing (Qs)mentioning

confidence: 99%

Smart biologics for crop protection in agricultural systems

Baysal¹,

Tör²

2014

Turk J Agric For

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Crop losses caused by insects, pests, and pathogens remain one of the major problems in sustainable agriculture. Environmental and health concerns regarding the overuse of pesticides, and the impacts of climate change on epidemics are immediate pressing issues. In addition, the breakdown of plant resistance by pathogen populations brings limitations to the genetic control of diseases. Biologics can be effective in all types of agricultural systems including organic, sustainable, and conventional. Beneficial microorganisms including Bacillus and Trichoderma species have been employed as environmentally safe biopesticides. Molecular and proteomic studies on biopesticides have revealed the nature of antibiotics, secreted enzymes, and inhibitory compounds. This review focuses on the current knowledge regarding biological agents and their metabolites including quorum-sensing molecules and volatile compounds, and how they can be used in pest and disease management programs.

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A Rhodococcus qsdA -Encoded Enzyme Defines a Novel Class of Large-Spectrum Quorum-Quenching Lactonases

Cited by 176 publications

References 52 publications

The Multiple Signaling Systems Regulating Virulence in Pseudomonas aeruginosa

The Multiple Signaling Systems Regulating Virulence in Pseudomonas aeruginosa

Mini-review: Inhibition of biofouling by marine microorganisms

Smart biologics for crop protection in agricultural systems

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