A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers

Figarol, Agathe; Gibot, Laure; Golzio, Muriel; Lonetti, Barbara; Mingotaud, Anne‐Françoise; Rols, Marie‐Pierre

doi:10.1080/10717544.2018.1510064

Cited by 16 publications

(12 citation statements)

References 54 publications

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“…For an ultrastructural examination of organelles, cells were grown in monolayer on round glass coverslips. Since cells are not supposed to grow on glass, these coverslips were coated with gelatin (Fisher Scientific) chosen because of its high biocompatibility with cells [49]. Then 5 min after calcium or magnesium electroporation, coverslips were plunged in 2% glutaraldehyde in 0.1 M Sorensen phosphate buffer (pH 7.4) and then further processed for transmission electron microscopy.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Calcium Delivery by Electroporation Induces In Vitro Cell Death through Mitochondrial Dysfunction without DNA Damages

Gibot

Montigny

Baaziz

et al. 2020

Cancers

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Adolescent cancer survivors present increased risks of developing secondary malignancies due to cancer therapy. Electrochemotherapy is a promising anti-cancer approach that potentiates the cytotoxic effect of drugs by application of external electric field pulses. Clinicians proposed to associate electroporation and calcium. The current study aims to unravel the toxic mechanisms of calcium electroporation, in particular if calcium presents a genotoxic profile and if its cytotoxicity comes from the ion itself or from osmotic stress. Human dermal fibroblasts and colorectal HCT-116 cell line were treated by electrochemotherapy using bleomycin, cisplatin, calcium, or magnesium. Genotoxicity, cytotoxicity, mitochondrial membrane potential, ATP content, and caspases activities were assessed in cells grown on monolayers and tumor growth was assayed in tumor spheroids. Results in monolayers show that unlike cisplatin and bleomycin, calcium electroporation induces cell death without genotoxicity induction. Its cytotoxicity correlates with a dramatic fall in mitochondrial membrane potential and ATP depletion. Opposite of magnesium, over seven days of calcium electroporation led to spheroid tumor growth regression. As non-genotoxic, calcium has a better safety profile than conventional anticancer drugs. Calcium is already authorized by different health authorities worldwide. Therefore, calcium electroporation should be a cancer treatment of choice due to the reduced potential of secondary malignancies.

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Section: Transmission Electron Microscopymentioning

confidence: 99%

Calcium Delivery by Electroporation Induces In Vitro Cell Death through Mitochondrial Dysfunction without DNA Damages

Gibot

Montigny

Baaziz

et al. 2020

Cancers

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“…Therefore, micelles have become a good choice for enhancing the solubility of DTX in the blood. Among nanoparticles, polyethylene oxide (PEO) and polycaprolactone (PCL) are two common low-toxicity polymer biomaterials that are hydrophilic and hydrophobic, respectively [ 7 , 8 ]. Polymerization on acetal-PEO using the ε-caprolactone monomer forms a long chain structure, acetal-PEO- b -PCL (A-PEO-PCL) [ 9 ], and DTX can be entrapped in the core after micelle self-assembly formation.…”

Section: Introductionmentioning

confidence: 99%

Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer

Zhang

Wang

et al. 2021

J Nanobiotechnol

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Background Chemotherapy and gene therapy are used in clinical practice for the treatment of castration-resistant prostate cancer. However, the poor efficiency of drug delivery and serious systemic side effects remain an obstacle to wider application of these drugs. Herein, we report newly designed PEO-PCL micelles that were self-assembled and modified by spermine ligand, DCL ligand and TAT peptide to carry docetaxel and anti-nucleostemin siRNA. Results The particle size of the micelles was 42 nm, the zeta potential increased from − 12.8 to 15 mV after grafting with spermine, and the optimal N/P ratio was 25:1. Cellular MTT experiments suggested that introduction of the DCL ligand resulted in high toxicity toward PSMA-positive cells and that the TAT peptide enhanced the effect. The expression of nucleostemin was significantly suppressed in vitro and in vivo, and the tumour-inhibition experiment showed that the dual-drug delivery system suppressed CRPC tumour proliferation. Conclusions This targeted drug delivery system inhibited the G1/S and G2/M mitotic cycle via synergistic interaction of chemotherapeutics and gene drugs.

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“…Electroporation application to cell monolayers. Cells were grown either on gelatin-coated round 1cm glass coverslip [30]…”

Section: Methodsmentioning

confidence: 99%

Electroporation does not affect human dermal fibroblast proliferation and migration properties directly but indirectly via the secretome

Gouarderes

Doumard

Vicendo

et al. 2020

Bioelectrochemistry

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Aesthetic wound healing is often experienced by patients after electrochemotherapy. We hypothesized that pulsed electric fields applied during electrochemotherapy (ECT) or gene electrotransfer (GET) protocols could stimulate proliferation and migration of human cutaneous cells, as described in protocols for electrostimulation of wound healing. We used videomicroscopy to monitor and quantify in real time primary human dermal fibroblast behavior when exposed in vitro to ECT and GET electric parameters, in terms of survival, proliferation and migration in a calibrated scratch wound assay. Distinct electric field intensities were applied to allow gradient in cell electropermeabilization while maintaining reversible permeabilization conditions, in order to mimic in vivo heterogeneous electric field distribution of complex tissues. Neither galvanotaxis nor statistical modification of fibroblast migration were observed in a calibrated scratch wound assay after application of ECT and GET parameters. The only effect on proliferation was observed under the strongest GET conditions, which drastically reduced the number of fibroblasts through induction of mitochondrial stress and apoptosis. Finally, we found that 24h-conditioned cell culture medium by electrically stressed fibroblasts tended to increase the migration properties of cells that were not exposed to electric field. RT-qPCR array indicated that several growth factor transcripts were strongly modified after electroporation.

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A journey from the endothelium to the tumor tissue: distinct behavior between PEO-PCL micelles and polymersomes nanocarriers

Cited by 16 publications

References 54 publications

Calcium Delivery by Electroporation Induces In Vitro Cell Death through Mitochondrial Dysfunction without DNA Damages

Calcium Delivery by Electroporation Induces In Vitro Cell Death through Mitochondrial Dysfunction without DNA Damages

Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer

Electroporation does not affect human dermal fibroblast proliferation and migration properties directly but indirectly via the secretome

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