A K-17 serotype specific Klebsiella phage JKP2 with biofilm reduction potential

Asif, Muhammad; Alvi, Iqbal Ahmad; Waqas, Muhammad; Basit, Abdul; Raza, Faisal; Rehman, Shafiq-Ur

doi:10.1016/j.virusres.2023.199107

Cited by 3 publications

(1 citation statement)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyvalent phage KL-2146 can infect NDM producing Klebsiella and antibiotic-sensitive K. pneumoniae 13, 883, which was demonstrated to effectively disrupt biofilms possessing multiple Klebsiella strains (Gilcrease et al, 2023). Asif et al isolated a K-17 serotype specific K. pneumoniae phage JKP2, which could significantly eliminate preformed biofilms with a rate of 98% for 24-hour-old biofilm, 96% of 48-hour-old biofilm, 86% and 82% for mature biofilm on day 3 and 4, respectively (Asif et al, 2023). Rahimi et al characterized a lytic phage PSKP16 with therapeutic potential against b-lactamase and biofilm producing K2-Hypervirulent K. pneumoniae using a mouse pneumonia model (Rahimi et al, 2023).…”

Section: Phages In the Research Stagementioning

confidence: 99%

Relationship between biofilm formation and antibiotic resistance of Klebsiella pneumoniae and updates on antibiofilm therapeutic strategies

Li,

Gao,

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Klebsiella pneumoniae is a Gram-negative bacterium within the Enterobacteriaceae family that can cause multiple systemic infections, such as respiratory, blood, liver abscesses and urinary systems. Antibiotic resistance is a global health threat and K. pneumoniae warrants special attention due to its resistance to most modern day antibiotics. Biofilm formation is a critical obstruction that enhances the antibiotic resistance of K. pneumoniae. However, knowledge on the molecular mechanisms of biofilm formation and its relation with antibiotic resistance in K. pneumoniae is limited. Understanding the molecular mechanisms of biofilm formation and its correlation with antibiotic resistance is crucial for providing insight for the design of new drugs to control and treat biofilm-related infections. In this review, we summarize recent advances in genes contributing to the biofilm formation of K. pneumoniae, new progress on the relationship between biofilm formation and antibiotic resistance, and new therapeutic strategies targeting biofilms. Finally, we discuss future research directions that target biofilm formation and antibiotic resistance of this priority pathogen.

show abstract

Section: Phages In the Research Stagementioning

confidence: 99%

Relationship between biofilm formation and antibiotic resistance of Klebsiella pneumoniae and updates on antibiofilm therapeutic strategies

Li,

Gao,

et al. 2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

Efficacy of a new K3-specific bacteriophage for controlling Klebsiella pneumoniae in milk and its potential to disrupt biofilm formation

Li,

Zhou,

Guo

et al. 2024

Food Control

View full text Add to dashboard Cite

Characterization of newly isolated bacteriophages targeting carbapenem-resistantKlebsiella pneumoniae

Kim,

Choi

et al. 2024

Preprint

View full text Add to dashboard Cite

Klebsiella pneumoniae, a Gram-negative opportunistic pathogen, is increasingly resistant to carbapenems in clinical settings. This growing problem necessitates the development of alternative antibiotics, with phage therapy being one promising option. In this study, we investigated novel phages targeting carbapenem-resistant Klebsiella pneumoniae (CRKP) and evaluated their lytic capacity against clinical isolates of CRKP. First, 23 CRKP clinical isolates were characterized using Multi-locus Sequence Typing (MLST), carbapenemase test, string test, and capsule typing. MLST classified the 23 K. pneumoniae isolates into 10 sequence types (STs), with the capsule types divided into nine known and one unknown type. From sewage samples collected from a tertiary hospital, 38 phages were isolated. Phenotypic and genotypic characterization of these phages was performed using Random Amplification of Polymorphic DNA-PCR (RAPD-PCR), transmission electron microscopy (TEM), and whole genome sequencing (WGS) analysis. Host spectrum analysis revealed that each phage selectively lysed strains sharing the same STs as their hosts, indicating ST-specific activity. These phages were subtyped based on their host spectrum and RAPD-PCR, identifying nine and five groups, respectively. Fourteen phages were selected for further analysis using TEM and WGS, revealing 13 Myoviruses and one Podovirus. Genomic analysis grouped the phages into three clusters: one closely related to Alcyoneusvirus, one to Autographiviridae, and others to Straboviridae. Our results showed that the host spectrum of K. pneumoniae-specific phages corresponds to the STs of the host strain. These 14 novel phages also hold promise as valuable resources for phage therapy against CRKP.

show abstract

A K-17 serotype specific Klebsiella phage JKP2 with biofilm reduction potential

Cited by 3 publications

References 71 publications

Relationship between biofilm formation and antibiotic resistance of Klebsiella pneumoniae and updates on antibiofilm therapeutic strategies

Relationship between biofilm formation and antibiotic resistance of Klebsiella pneumoniae and updates on antibiofilm therapeutic strategies

Efficacy of a new K3-specific bacteriophage for controlling Klebsiella pneumoniae in milk and its potential to disrupt biofilm formation

Characterization of newly isolated bacteriophages targeting carbapenem-resistantKlebsiella pneumoniae

Contact Info

Product

Resources

About