A Key Gene, PLIN1, Can Affect Porcine Intramuscular Fat Content Based on Transcriptome Analysis

Li, Bojiang; Weng, Qiannan; Dong, Chao; Zhang, Zengkai; Li, Rongyang; Liu, Jingge; Jiang, Aiwen; Li, Qifa; Jia, Chao; Wu, Wangjun

doi:10.3390/genes9040194

Cited by 58 publications

(37 citation statements)

References 68 publications

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“…A previous study conducting expression profiling of porcine adipose tissue suggested CYBB to play a role in fat metabolism and adipogenic differentiation [ 74 ]. Correlation analysis has also revealed Perilipin 1 ( PLIN 1 ; fold change = -1.42) to be positively correlated with IMF, SFA, MUFA and PUFA and this is in keeping with a previous study reporting its higher abundance being associated with increased IMF in porcine muscle [ 75 ]. Moreover, C-X-C motif chemokine ligand 10 ( CXCL10 , fold change = 2.24), which was previously associated to marbling in cattle [ 76 ], was negatively correlated with IMF, SFA and MUFA.…”

Section: Discussionsupporting

confidence: 88%

RNA-seq of muscle from pigs divergent in feed efficiency and product quality identifies differences in immune response, growth, and macronutrient and connective tissue metabolism

et al. 2018

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BackgroundFeed efficiency (FE) is an indicator of efficiency in converting energy and nutrients from feed into a tissue that is of major environmental and economic significance. The molecular mechanisms contributing to differences in FE are not fully elucidated, therefore the objective of this study was to profile the porcine Longissimus thoracis et lumborum (LTL) muscle transcriptome, examine the product quality from pigs divergent in FE and investigate the functional networks underpinning the potential relationship between product quality and FE.ResultsRNA-Seq (n = 16) and product quality (n = 40) analysis were carried out in the LTL of pigs differing in FE status. A total of 272 annotated genes were differentially expressed with a P < 0.01. Functional annotation revealed a number of biological events related to immune response, growth, carbohydrate & lipid metabolism and connective tissue indicating that these might be the key mechanisms governing differences in FE. Five most significant bio-functions altered in FE groups were ‘haematological system development & function’, ‘lymphoid tissue structure & development’, ‘tissue morphology’, ‘cellular movement’ and ‘immune cell trafficking’. Top significant canonical pathways represented among the differentially expressed genes included ‘IL-8 signalling’, ‘leukocyte extravasation signalling, ‘sphingosine-1-phosphate signalling’, ‘PKCθ signalling in T lymphocytes’ and ‘fMLP signalling in neutrophils’. A minor impairment in the quality of meat, in relation to texture and water-holding capacity, produced by high-FE pigs was observed. High-FE pigs also had reduced intramuscular fat content and improved nutritional profile in terms of fatty acid composition.ConclusionsOntology analysis revealed enhanced activity of adaptive immunity and phagocytes in high-FE pigs suggesting more efficient conserving of resources, which can be utilised for other important biological processes. Shifts in carbohydrate conversion into glucose in FE-divergent muscle may underpin the divergent evolution of pH profile in meat from the FE-groups. Moreover, altered amino acid metabolism and increased mobilisation & flux of calcium may influence growth in FE-divergent muscle. Furthermore, decreased degradation of fibroblasts in FE-divergent muscle could impact on collagen turnover and alter tenderness of meat, whilst enhanced lipid degradation in high-FE pigs may potentially underlie a more efficient fat metabolism in these animals.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5175-y) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 88%

RNA-seq of muscle from pigs divergent in feed efficiency and product quality identifies differences in immune response, growth, and macronutrient and connective tissue metabolism

et al. 2018

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“…The effect of PPARβ/δ is similar to those of PPARα, which promotes energy dissipation, but opposite to those of PPARγ, which promotes energy storage [33]. PLIN1 was found to promote lipid droplet formation by activating the PPARγ signaling pathway [34].…”

Section: Ppar Pathway Regulates Weakened Lipolysis and Enhanced Lipogmentioning

confidence: 94%

“…Native PLIN1 may serve as a barrier, protecting droplet triglycerides from basal lipolysis, whereas phosphorylated PLIN1 could indirectly activate ATGL [36,37]. Previous studies have found that the expression of PLIN1 was higher in muscles with high IMF contents [34,38]. In addition, AFABP, a negative regulator of mitochondrial fatty acid oxidation [39], was also upregulated in the F line.…”

Section: Ppar Pathway Regulates Weakened Lipolysis and Enhanced Lipogmentioning

confidence: 95%

Effect of Divergent Selection for Intramuscular Fat Content on Muscle Lipid Metabolism in Chickens

Liu

Cui

Xing

et al. 2019

Animals

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Simple Summary: Intramuscular fat is an important factor affecting meat quality and consumer acceptance. Appropriate increases in the intramuscular fat content contribute to the improvement of meat quality, and genetic selection is an effective method to increase the intramuscular fat content in chickens. In this study, chicken lines divergently selected for their intramuscular fat content were used to investigate the mechanisms behind differential intramuscular fat deposition. These results found in this study may contribute to the improvement of meat quality in chickens.Abstract: Intramuscular fat (IMF)-an important factor affecting meat quality-can be appropriately increased by genetic selection. Chicken lines divergently selected for IMF content were used in this study to investigate the mechanisms behind differential IMF deposition. Sixty 15th generation chickens were genotyped using the IASCHICK 55K single nucleotide polymorphism (SNP) chip. After quality control, 59 chickens and 36,893 SNPs were available for subsequent analysis. Population structure assessment indicated that the lines were genetically differentiated. Based on the top 1% paired fixation index values, three pathways were significantly (p < 0.05) enriched, and nine genes were considered candidate genes for differential IMF deposition. Differences between the lines in the expressions of representative genes involved in the above pathways were detected in 16th generation chickens. This study suggests that genetic selection for increased IMF in the pectoralis major muscle may enhance fatty acid synthesis, transport, and esterification, and reduce triglyceride hydrolysis. The peroxisome proliferator-activated receptor (PPAR) signaling pathway, glycerolipid metabolism, and fatty acid degradation pathway may have contributed to the differences in IMF deposition between the lines. These results contribute to the understanding of the genetic mechanisms behind IMF deposition, and the improvement of chicken meat quality.

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“…Among these DE genes, downregulated in Iberian LBW animals, Perilipin-1 (PLIN1) is a key gene for porcine adipogenesis, and is closely related to the accumulation of intramuscular fat [87]. LBW Iberian fetuses also exhibited downregulation of CIDEC, which has been linked to adipocyte differentiation and metabolic disorders in humans [88], and which downregulates AMPKα and promotes adipogenesis [89].…”

Section: Foxo1 Foxo3 Foxo6 Col11a2 Myod Igf-i Fos) Protein Catamentioning

confidence: 99%

Impact of genotype, body weight and sex on the prenatal muscle transcriptome of Iberian pigs

et al. 2020

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Growth is dependent on genotype and diet, even at early developmental stages. In this study, we investigated the effects of genotype, sex, and body weight on the fetal muscle transcriptome of purebred Iberian and crossbred Iberian x Large White pigs sharing the same uterine environment. RNA sequencing was performed on 16 purebred and crossbred fetuses with high body weight (340±14g and 415±14g, respectively) and 16 with low body weight (246±14g and 311±14g, respectively), on gestational day 77. Genotype had the greatest effect on gene expression, with 645 genes identified as differentially expressed (DE) between purebred and crossbred animals. Functional analysis showed differential regulation of pathways involved in energy and lipid metabolism, muscle development, and tissue disorders. In purebred animals, fetal body weight was associated with 35 DE genes involved in development, lipid metabolism and adipogenesis. In crossbred animals, fetal body weight was associated with 60 DE genes involved in muscle development, viability, and immunity. Interestingly, the results suggested an interaction genotype*weight for some DE genes. Fetal sex had only a modest effect on gene expression. This study allowed the identification of genes, metabolic pathways, biological functions and regulators related to fetal genotype, weight and sex, in animals sharing the same uterine environment. Our findings contribute to a better understanding of the molecular events that influence prenatal muscle development and highlight the complex interactions affecting transcriptional regulation during development.

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A Key Gene, PLIN1, Can Affect Porcine Intramuscular Fat Content Based on Transcriptome Analysis

Cited by 58 publications

References 68 publications

RNA-seq of muscle from pigs divergent in feed efficiency and product quality identifies differences in immune response, growth, and macronutrient and connective tissue metabolism

RNA-seq of muscle from pigs divergent in feed efficiency and product quality identifies differences in immune response, growth, and macronutrient and connective tissue metabolism

Effect of Divergent Selection for Intramuscular Fat Content on Muscle Lipid Metabolism in Chickens

Impact of genotype, body weight and sex on the prenatal muscle transcriptome of Iberian pigs

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