A kinetic modelling approach to study the role of energy regulatory pathways in CHO cells

Ghorbaniaghdam, Atefeh; Henry, Olivier; Jolicœur, Mario

doi:10.3182/20100707-3-be-2012.0109

Cited by 1 publication

(2 citation statements)

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“…Glycolysis accounts for 45% of the ATP generated in the cell during the exponential growth phase, 23 resulting in, when combined with mitochondrial ATP export, higher cytosolic concentrations of ATP than the mitochondria. 24,25 Consistent with the Warburg Effect, the CHO cells have the highest glucose uptake rate 7,26 and glycolytic flux 4,23,25,[27][28][29] through glucose-6-phosphate isomerase (GPI) during the exponential growth phase compared to other growth phases. Elevated lactate dehydrogenase (LDH) flux is generally observed in cells demonstrating the Warburg effect, as this regenerates the NAD + from NADH necessary for glycolysis.…”

Section: Trends In Glycolysismentioning

confidence: 82%

“…54,55 The reader is directed toward the recent review by Hartley et al for a more comprehensive discussion on lactate consumption in CHO cells. 53 Similar to lactate, several other metabolites may show a shift from production to consumption in the stationary phase including alanine, 23,37,41,56 ammonia, 5,27,30 and glycine. 30,56 It is important to note that any metabolic shift from production to consumption is not a universal phenomenon among CHO cells.…”

Section: Trends At the Pyruvate Branch Pointmentioning

confidence: 99%

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Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions

Rish

Drennen

Anderson

2021

Biotechnology Progress

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Extensive knowledge of Chinese hamster ovary (CHO) cell metabolism is required to improve process productivity and culture performance in biopharmaceutical manufacturing. However, CHO cells show a dynamic metabolism during culturing in batch and fed-batch bioreactors. CHO cell metabolism is generally described as taking place in three stages: exponential growth phase, stationary phase, and death phase. This review aims to summarize the trends of central metabolism for CHO cells during each stage. Additional insights into how culture conditions are related to phase transitions and force metabolic rewiring are provided. Understanding of CHO cell metabolism lends itself to improving culture qualities by, for example, identifying sources of toxic byproducts and pathways for cellular engineering. In summary, this review describes the changes in CHO cell central metabolism over the course of the culture.

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