“…The biological removal of nitrate can be affected by various factors: different types of external C sources [6,7], various types of micro-organisms [6,8], various operational parameters such as C/N ratios [2,9,10], temperature [3,[11][12][13], pH [3,11], dissolved oxygen [12,14,15], hydraulic retention time [16][17][18], nitrate and nitrite concentratios [11,19] and mixed liquor suspended solids [10,20]. In addition, nitrate removal highly depends on the substrate amount that influences the denitrification rate, denitrification yield, and the composition of the microflora [6,21].…”