A label-free and enzyme-free fluorescent assay for mercury ions based on T-Hg(II)-T nanoladders and DNA-templated silver nanoclusters/graphene oxide nanocomposites

Label-Free Fluorescence Sensing Strategy Based on Functional Nucleic Acids via Energy Transfer between DNA-Templated Silver Nanoclusters and Gold Nanorods

“…The fluorescent probe DNA-AgNCs were synthesized according to our previous method . Here, DNA-AgNCs synthesized by cDNA strands and substrate strands are named as cDNA-AgNCs and sub-AgNCs, respectively.…”

Fluorescent Aptasensors: Design Strategies and Applications in Analyzing Chemical Contamination of Food

“…Several nonimmobilized fluorescent Hg 2+ nanoaptasensors have been designed using QD probes , or DNA templated AgNCs (DNA-AgNCs) . Exploiting aptamer-programmed self-assembly of functionalized CdTe QDs, Guo et al proposed a fluorescent nanoaptasensor, in which two pieces of T-rich aptamer sequences were attached to CdTe QDs .…”

“…Different from the common sensing method of synthesis followed by functionalization, Hg 2+ detection was simultaneously accomplished during the synthesis of QDs with an LOD of 0.5 nM (∼0.1 ng mL –1 ). Recently, by skillfully combining T-Hg 2+ -T nanoladders as signal amplification and DNA-AgNCs as signal identification, Wang et al constructed a label-free and enzyme-free turn-on biosensor for Hg 2+ ions . DNA-AgNCs were successfully synthesized using the DNA template with a sequence of C 6 G 5 C 6 that extended at the 5′-end of the DNA probe.…”

Multifunctional dual emissive fluorescent probe based on europium-doped carbon dots (Eu-TCA/NCDs) for highly selective detection of chloramphenicol, Hg2+ and Fe3+

“…11,12 As a result, the development of better technologies for detecting these environmental pollutants is critical. To date, numerous analytical procedures, consisting of liquid chromatographymass spectrometry, liquid/gas chromatography-tandem mass spectrometry, fluorescence assays, electrochemical sensing, and immunoassay biosensing, 5,[13][14][15][16][17][18][19][20] have been reported in previous studies for selective detection of CAP, Hg 2+ and Fe 3+ . Although all these methods show exceptional precision and accuracy for detection, inevitable hurdles, including expensive instrumentation, time-consuming multi-step sample pre-treatment processes, antibody instability, the elevated oxidation potential of an electrochemical sensor, precipitation of fluorescent organic molecules under aqueous conditions, etc., make them less appealing for on-field application.…”