A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

Du, Gaoshang; Zhang, Dongwei; Xia, Bing; Xu, Lurong; Wu, Shijian; Zhan, Shenshan; Ni, Xuan; Zhou, Xiaotong; Wang, Lumei

doi:10.1007/s00604-016-1861-0

Cited by 35 publications

(10 citation statements)

References 36 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…25 Of the various reported estradiol aptamers, those developed by Kim et al 26 and Alsager et al 27 have been widely adopted in various applications. 28−31 In the case of progesterone, only one aptamer has been reported to date, 32 which has been exploited 33,34 despite the fact that it cross reacts with other steroids. 33−35 Finally, testosterone-binding aptamers have been reported 36 but to date, no application of this aptamer has been reported.…”

Section: Introductionmentioning

confidence: 99%

“…28−31 In the case of progesterone, only one aptamer has been reported to date, 32 which has been exploited 33,34 despite the fact that it cross reacts with other steroids. 33−35 Finally, testosterone-binding aptamers have been reported 36 but to date, no application of this aptamer has been reported. The structural similarities between steroids are quite high thus obtaining aptamers with high specificity for these small molecules is a challenging task.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection

et al. 2019

View full text Add to dashboard Cite

Aptamers are well-established biorecognition molecules used in a wide variety of applications for the detection of their respective targets. However, individual SELEX processes typically performed for the identification of aptamers for each target can be quite time-consuming, labor-intensive, and costly. An alternative strategy is proposed herein for the simultaneous identification of different aptamers binding distinct but structurally similar targets in one single selection. This one-pot SELEX approach, using the steroids estradiol, progesterone, and testosterone as model targets, was achieved by combining the benefits of counter-SELEX with the power of next-generation sequencing and bioinformatics analysis. The pools from the last stage of the selection were compared in order to discover sequences with preferential abundance in only one of the pools. This led to the identification of aptamer candidates with potential specificity to a single steroid target. Binding studies demonstrated the high affinity of each selected aptamer for its respective target, and low nanomolar range dissociation constants calculated were similar to those previously reported for steroid-binding aptamers selected using traditional SELEX approaches. Finally, the selected aptamers were exploited in microtiter plate assays, achieving nanomolar limits of detection, while the specificity of these aptamers was also demonstrated. Overall, the one-pot SELEX strategy led to the discovery of aptamers for three different steroid targets in one single selection without compromising their affinity or specificity, demonstrating the power of this approach of aptamer discovery for the simultaneous selection of aptamers against multiple targets.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Du et al . 17 covered specific aptamers of gonadotropin progesterone (P4) on nanogold surface and lead to the dispersion of AuNPs in NaCl solution. When P4 was present, it can be combined with an aptamer to form a stable complex structure, which releases AuNPs to aggregate in solution.…”

Section: Introductionmentioning

confidence: 99%

A dual-model SERS and RRS analytical platform for Pb(II) based on Ag-doped carbon dot catalytic amplification and aptamer regulation

Wang

Huang

Wen

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Several carbon dots doping with diferent elements (Ca, Ag, Au) were fabricated and their catalytic properties had been investigated in this paper. It was found that the Ag-doped carbon dots (CD Ag ) had played a role of mimic enzyme on the reaction of HAuCl 4 -H 2 O 2 and generated nanogold particles with surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) effects. The aptamer (Apt) can be adsorbed on the CD Ag surface and cause the catalysis weakening. When the target Pb(II) was added, it would combine with the Apt to produce firm complexes Pb-Apt and desorb CD Ag , which caused its catalytic effect restore. The formed nanogold had a strong RRS peak (at 375 nm) and a high SERS peak (at 1615 cm −1 ) in the presence of molecular probe (Victoria blue B, VBB). The dual-model signals of SERS and RRS increased linearly with Pb(II) concentration increase within the scope of 0.006–0.46 μmol/L and 0.01–0.46 μmol/L. And their detection limits respectively were 0.0032 μmol/L and 0.0048 μmol/L Pb(II).

show abstract

“…Using Apt-modified metal nanoparticles, analyses such as sensitive Apt nanophotometry, fluorescence methods, resonance Rayleigh scattering, and surface enhanced Raman scattering (SERS) were conducted [ 1 , 2 , 3 ]. Du et al [ 4 ] prepared a gonadotropin progesterone (P4) Apt-gold nanoparticle colorimetric sensor with a detection range of 2.6–1400 nmol/L P4 and a detection limit of 2.6 nmol/L. Ma et al [ 5 ] obtained a stable tobramycin Apt-nanogold resonance Rayleigh scattering (RRS) probe by using tobramycin (TBC) Apt-modified nanogold with a detect range of 1.9–58.3 ng/mL TBC and a detection limit of 0.8 ng/mL.…”

Section: Introductionmentioning

confidence: 99%

Using N-doped Carbon Dots Prepared Rapidly by Microwave Digestion as Nanoprobes and Nanocatalysts for Fluorescence Determination of Ultratrace Isocarbophos with Label-Free Aptamers

Jiang

Liu

et al. 2019

Nanomaterials

View full text Add to dashboard Cite

The strongly fluorescent and highly catalytic N-doped carbon dots (CDN) were rapidly prepared by a microwave irradiation procedure and were characterized by electron microscopy (EM), laser scattering, infrared spectroscopy (IR), and by their fluorescence spectrum. It was found that the CDN had a strong catalytic effect on the fluorescence reaction of 3,3′,5,5′-tetramethylbenzidine hydroxide ((TMB)–H2O2) which produced the oxidation product of TMB (TMBOX) with strong fluorescence at 406 nm. The aptamer (Apt) was adsorbed on the CDN surfaces which weakened the fluorescence intensity due to the inhibition of catalytic activity. When the target molecule isocarbophos (IPS) was added, it reacted with the Apt to form a stable conjugate and free CDN which restored the catalytic activity to enhance the fluorescence. Using TMBOX as a fluorescent probe, a highly sensitive nanocatalytic method for determination of 0.025–1.5 μg/L IPS was established with a detection limit of 0.015 μg/L. Coupling the CDN fluorescent probe with the Apt–IPS reaction, a new CD fluorescence method was established for the simple and rapid determination of 0.25–1.5 μg/L IPS with a detection limit of 0.11 μg/L.

show abstract

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

Cited by 35 publications

References 36 publications

One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection

One-Pot SELEX: Identification of Specific Aptamers against Diverse Steroid Targets in One Selection

A dual-model SERS and RRS analytical platform for Pb(II) based on Ag-doped carbon dot catalytic amplification and aptamer regulation

Using N-doped Carbon Dots Prepared Rapidly by Microwave Digestion as Nanoprobes and Nanocatalysts for Fluorescence Determination of Ultratrace Isocarbophos with Label-Free Aptamers

Contact Info

Product

Resources

About