A label-free electrochemical immnunosensor based on signal magnification of oxygen reduction reaction catalyzed by uniform PtCo nanodendrites for highly sensitive detection of carbohydrate antigen 15-3

Ge, Xin-Yue; Cen, Shi-Yun; Wang, Ai‐Jun; Mei, Li-Ping; Liu, Xiang; Feng, Jiu‐Ju

doi:10.1016/j.aca.2021.338750

Cited by 29 publications

(14 citation statements)

References 56 publications

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“…Electrochemical-based biosensors can be exploited to achieve a very low limit of detection (LOD) in immunoassays. An ideal electrochemical immunosensor should enable high sample loading and long-term durability/reproducibility without affecting biological activity. , Recently, immunosensors gained much attention for clinical diagnosis as they can detect specific interaction between the target antigen and antibodies that form a stable immunocomplex. The antigen–antibody interaction generates measurable electrical signals in response to biomolecule concentration changes.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C₃N₄/ZnO Nanocomposite-Modified Au Electrode

Kumar

Chauhan

et al. 2022

ACS Omega

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A g-C 3 N 4 /ZnO (graphitic carbon nitride/zinc oxide) nanocomposite-decorated gold electrode was employed to design an antigen–antibody-based electrochemical biosensor to detect Helicobacter pylori specific toxin, vacuolating cytotoxin A (VacA). The thermal condensation method was used to synthesize the g-C 3 N 4 /ZnO nanocomposite, and the nanocomposite was deposited electrochemically on a gold electrode. The morphology as well as the structure of the synthesized nanocomposite were confirmed by scanning electron microscopy, energy-dispersive X-ray analysis, X-ray diffraction, and Fourier transform infrared techniques. The nanocomposite efficiently increased the sensor performance by amplifying the signals. EDC-NHS chemistry was exploited for attachment of VacA antibodies covalently with the g-C 3 N 4 /ZnO-modified gold electrode. This modified electrode was exploited for immunosensing of H. pylori -specific VacA antigen. The immunosensor was stable for up to 30 days and exhibited good sensitivity of 0.3 μA –1 ng mL –1 in a linear detection range of 0.1 to 12.8 ng mL –1 . Apart from this, the fabricated sensor showed unprecedented reproducibility and remarkable selectivity toward the H. pylori toxin VacA. Thus, the highly sensitive immunosensor is a desirable platform for H. pylori detection in practical applications and clinical diagnosis.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C₃N₄/ZnO Nanocomposite-Modified Au Electrode

Kumar

Chauhan

et al. 2022

ACS Omega

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“…Among the fatal cancers that cause patients’ death, pancreatic cancer is a prominent one, leaving only a 5% survival chance. , The carbohydrate antigen 19-9 (CA19-9) has played a critical role as a biomarker to help patients with gastrointestinal malignancies mostly suffering from pancreatic cancer . Among the different types of implemented biomarkers for this disease, the carbohydrate antigen 15-3 (CA15-3) has brought more hope, offering comprehensive information needed for breast cancer detection and therapy. − In the past decade, microRNA, a non-coding RNA molecule, has found a secure place in novel diagnostic methods for incurable diseases. Overexpression of microRNA-155 has been observed in breast cancer patients.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Role of 2D-Graphdiyne as a Charge Carrier Layer in Field-Effect Transistors for Non-Covalent Biological Immobilization against Human Diseases

Ghafary

Salimi

Hallaj

2022

ACS Biomater. Sci. Eng.

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Graphdiyne's (GDY's) outstanding features have made it a novel 2D nanomaterial and a great candidate for electronic gadgets and optoelectronic devices, and it has opened new opportunities for the development of highly sensitive electronic and optical detection methods as well. Here, we testified a non-covalent grafting strategy in which GDY serves as a charge carrier layer and a bioaffinity substrate to immobilize biological receptors on GDY-based field-effect transistor (FET) devices. Firm non-covalent anchoring of biological molecules via pyrene groups and electrostatic interactions in addition to preserved electrical properties of GDY endows it with features of an ultrasensitive and stable detection mechanism. With emerging new forms and extending the subtypes of the already existing fatal diseases, genetic and biological knowledge demands more details. In this regard, we constructed simple yet efficient platforms using GDY-based FET devices in order to detect different kinds of biological molecules that threaten human health. The resulted data showed that the proposed non-covalent bioaffinity assays in GDY-based FET devices could be considered reliable strategies for novel label-free biosensing platforms, which still reach a high on/off ratio of over 10 4 . The limits of detection of the FET devices to detect DNA strands, the CA19-9 antigen, microRNA-155, the CA15-3 antigen, and the COVID-19 antigen were 0.2 aM, 0.04 pU mL −1 , 0.11 aM, 0.043 pU mL −1 , and 0.003 fg mL −1 , respectively, in the linear ranges of 1 aM to 1 pM, 1 pU mL −1 to 0.1 μU mL −1 , 1 aM to 1 pM, 1 pU mL −1 to 10 μU mL −1 , and 1 fg mL −1 to 10 ng mL −1 , respectively. Finally, the extraordinary performance of these label-free FET biosensors with low detection limits, high sensitivity and selectivity, capable of being miniaturized, and implantability for in vivo analysis makes them a great candidate in disease diagnostics and pointof-care testing.

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“…20,26,27,[29][30][31][32][33][34] Recently, dendrite-like architectures of HMNMs have attracted substantial attention because of their appealing properties, owing to more steps, edges and corners that would create more active sites facilely available. 26,35,36 For example, Pan and co-workers developed PEGylated Au@Pt nanodendrites as an enhanced theranostic agent for CT imaging and photothermal/radiation therapy (Fig. 2C).…”

Section: Morphologymentioning

confidence: 99%

“…152,153 To date, researchers have developed a variety of HMNM-based electrochemical, FL and colorimetric biosensors to detect disease-related protein markers, such as tumor, hepatic, cardiac, inammatory markers, and others (Table 2). 24,30,35,36,46,47,[49][50][51][52]64,65,81,84,85,88,89,98,[101][102][103][107][108][109][110]114,122, 3.1.4 Nucleic acids. Circulating tumor DNA (ctDNA) is an informative cancer biomarker, which has genetic changes and the same mutations as primary tumor, even behaving as a "liquid biopsy" for early tumor discovery and hence avoiding traditional tissue biopsy.…”

Section: Sensingmentioning

confidence: 99%

Section: Applications Of Hmnms In Analytical Chemistrymentioning

confidence: 99%

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Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy

Wang

Yuan

et al. 2022

Chem. Sci.

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A label-free electrochemical immnunosensor based on signal magnification of oxygen reduction reaction catalyzed by uniform PtCo nanodendrites for highly sensitive detection of carbohydrate antigen 15-3

Cited by 29 publications

References 56 publications

Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C₃N₄/ZnO Nanocomposite-Modified Au Electrode

Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C₃N₄/ZnO Nanocomposite-Modified Au Electrode

Exploring the Role of 2D-Graphdiyne as a Charge Carrier Layer in Field-Effect Transistors for Non-Covalent Biological Immobilization against Human Diseases

Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy

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A label-free electrochemical immnunosensor based on signal magnification of oxygen reduction reaction catalyzed by uniform PtCo nanodendrites for highly sensitive detection of carbohydrate antigen 15-3

Cited by 29 publications

References 56 publications

Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C3N4/ZnO Nanocomposite-Modified Au Electrode

Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C3N4/ZnO Nanocomposite-Modified Au Electrode

Exploring the Role of 2D-Graphdiyne as a Charge Carrier Layer in Field-Effect Transistors for Non-Covalent Biological Immobilization against Human Diseases

Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy

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Product

Resources

About

Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C₃N₄/ZnO Nanocomposite-Modified Au Electrode

Electrochemical Immunosensor for Detection of H. pylori Secretory Protein VacA on g-C₃N₄/ZnO Nanocomposite-Modified Au Electrode