A label-free fiber ring laser biosensor for ultrahigh sensitivity detection of Salmonella Typhimurium

Qiu, Shi; Liu, Bin; Leng, Yuankui; Fox, Edward M.; Zhou, Xian; Yan, Binbin; Sang, Xinzhu; Long, Keping; Fu, Yanjun; He, Xingdao; Yuan, Jinhui; Farrell, Gerald; Wu, Qiang

doi:10.1016/j.bios.2023.115337

Cited by 15 publications

(5 citation statements)

References 44 publications

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“…These pathogens can invade human bodies through various unclean foods, causing abdominal cramps, fever, diarrhea, and even death. , Salmonella is one of the leading causes of food contamination, accounting for nearly 9,000 illnesses in Europe and 26,500 hospitalizations in the United States each year. , Salmonella is also the major cause of foodborne illness in China . Owing to its very low infectious dose and extremely high prevalence, official agencies around the world have set strict permissible thresholds for Salmonella residues. , The national standard of the People’s Republic of China has set the residue threshold of Salmonella in prepackaged products at 0 CFU/mL . Therefore, reliable early warning methods for Salmonella detection are urgently needed.…”

Section: Introductionmentioning

confidence: 99%

“…6 Owing to its very low infectious dose and extremely high prevalence, official agencies around the world have set strict permissible thresholds for Salmonella residues. 4,7 The national standard of the People's Republic of China has set the residue threshold of Salmonella in prepackaged products at 0 CFU/mL. 8 Therefore, reliable early warning methods for Salmonella detection are urgently needed.…”

Section: ■ Introductionmentioning

confidence: 99%

“…1 and 9−15) could induce differentiable SERS and colorimetric signals. In addition, the coexistence of other pathogens did not interfere with (6), P. aeruginosa (7), six nontarget strains (8), Salmonella + B. subtilis (9), Salmonella + E. coli (10), Salmonella + Sh. dysenteriae (11), Salmonella + S. aureus (12), Salmonella + C. ablicans (13), Salmonella + P. aeruginosa (14), Salmonella + six nontarget strains (15), and blank (16).…”

Section: ■ Introductionmentioning

confidence: 99%

“…(E) Specificity testing of dCas9-CSD with Salmonella (1), B. subtilis (2), E. coli (3), Sh. dysenteriae (4), S. aureus (5), C. ablicans(6), P. aeruginosa(7), six nontarget strains (8), Salmonella + B. subtilis (9), Salmonella + E. coli(10), Salmonella + Sh. dysenteriae(11), Salmonella + S. aureus(12), Salmonella + C. ablicans(13), Salmonella + P. aeruginosa(14), Salmonella + six nontarget strains(15), and blank(16).…”

mentioning

confidence: 99%

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Development of a Colorimetric and SERS Dual-Signal Platform via dCas9-Mediated Chain Assembly of Bifunctional Au@Pt Nanozymes for Ultrasensitive and Robust Salmonella Assay

Jiang,

Chang,

Zhu

et al. 2024

Anal. Chem.

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Timely screening for harmful pathogens is a great challenge in emergencies where traditional culture methods suffer from long assay time and alternative methods are limited by poor accuracy and low robustness. Herein, we present a dCas9-mediated colorimetric and surface-enhanced Raman scattering (SERS) dualsignal platform (dCas9-CSD) to address this challenge. Strategically, the platform used dCas9 to accurately recognize the repetitive sequences in amplicons produced by loop-mediated isothermal amplification (LAMP), forming nucleic acid frameworks that assemble numerous bifunctional gold−platinum (Au@Pt) nanozymes into chains on the surface of streptavidin-magnetic beads (SA-MB). The collected Au@Pt converted colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB) via its Pt shell and then enhanced the Raman signal of oxTMB by its Au core. Therefore, the presence of Salmonella could be dexterously converted into cross-validated colorimetric and SERS signals, providing more reliable conclusions. Notably, dCas9-mediated secondary recognition of amplicons reduced background signal caused by nontarget amplification, and two-round signal amplification consisting of LAMP reaction and Au@Pt catalysis greatly improved the sensitivity. With this design, Salmonella as low as 1 CFU/mL could be detected within 50 min by colorimetric and SERS modes. The robustness of dCas9-CSD was further confirmed by various real samples such as lake water, cabbage, milk, orange juice, beer, and eggs. This work provides a promising point-of-need tool for pathogen detection.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Development of a Colorimetric and SERS Dual-Signal Platform via dCas9-Mediated Chain Assembly of Bifunctional Au@Pt Nanozymes for Ultrasensitive and Robust Salmonella Assay

Jiang,

Chang,

Zhu

et al. 2024

Anal. Chem.

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“…Foodborne diseases have emerged as a growing global concern [1]. Salmonella, as the most widespread foodborne bacterial pathogen, has caused extensive food poisoning, illness, and death.…”

Section: Introductionmentioning

confidence: 99%

A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent Aptamer

Ma,

Sun,

Shi

et al. 2023

Foods

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Salmonella infection has emerged as a global health threat, causing death, disability, and socioeconomic disruption worldwide. The rapid and sensitive detection of Salmonella is of great significance in guaranteeing food safety. Herein, we developed a colorimetric/fluorescent dual-mode method based on a DNA-nanotriangle programmed multivalent aptamer for the sensitive detection of Salmonella. In this system, aptamers are precisely controlled and assembled on a DNA nanotriangle structure to fabricate a multivalent aptamer (NTri-Multi-Apt) with enhanced binding affinity and specificity toward Salmonella. The NTri-Multi-Apt was designed to carry many streptavidin-HRPs for colorimetric read-outs and a large load of Sybr green I in the dsDNA scaffold for the output of a fluorescent signal. Therefore, combined with the magnetic separation of aptamers and the prefabricated NTri-Multi-Apt, the dual-mode approach achieved simple and sensitive detection, with LODs of 316 and 60 CFU/mL for colorimetric and fluorescent detection, respectively. Notably, the fluorescent mode provided a self-calibrated and fivefold-improved sensitivity over colorimetric detection. Systematic results also revealed that the proposed dual-mode method exhibited high specificity and applicability for milk, egg white, and chicken meat samples, serving as a promising tool for real bacterial sample testing. As a result, the innovative dual-mode detection method showed new insights for the detection of other pathogens.

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Multimodal nanoenzyme-linked aptamer assay for Salmonella typhimurium based on catalysis and photothermal effect of PB@Au

Wang,

Liu,

Liu

et al. 2025

Microchim Acta

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A label-free fiber ring laser biosensor for ultrahigh sensitivity detection of Salmonella Typhimurium

Cited by 15 publications

References 44 publications

Development of a Colorimetric and SERS Dual-Signal Platform via dCas9-Mediated Chain Assembly of Bifunctional Au@Pt Nanozymes for Ultrasensitive and Robust Salmonella Assay

Development of a Colorimetric and SERS Dual-Signal Platform via dCas9-Mediated Chain Assembly of Bifunctional Au@Pt Nanozymes for Ultrasensitive and Robust Salmonella Assay

A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent Aptamer

Multimodal nanoenzyme-linked aptamer assay for Salmonella typhimurium based on catalysis and photothermal effect of PB@Au

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