A label-free fluorescence method based on terminal deoxynucleotidyl transferase and thioflavin T for detecting prostate-specific antigen

Chen, Mingjian; Ma, Changbei; Yan, Ying; Zhao, Han

doi:10.1007/s00216-019-01958-0

Cited by 22 publications

(8 citation statements)

References 43 publications

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“…This strategy can be used universally to any tumor type, including solid and hematologic tumors. An increasing number of functionally diverse and tumor-specific aptamers and checkpoint aptamers, such as tumor-specific MAGE-A 3111–125 aptamer, prostate-specific antigen aptamer, − Mucin1 (MUC1)-positive tumor cells aptamers, , tumor vasculatures endoglin aptamer, checkpoint inhibitors PD-1, PD-L1 aptamers, , and CTLA4 aptamer, are currently being screened via the SELEX method, thus expanding the application range of cancer immunotherapy by replacing scFv or monoclonal antibody. The limitation of the current strategy is that aptamer-T cells are activated and expanded in vitro ; after aptamer-T cell preparation, it cannot be amplified in vivo .…”

Section: Discussionmentioning

confidence: 99%

Aptamer-T Cell Targeted Therapy for Tumor Treatment Using Sugar Metabolism and Click Chemistry

et al. 2020

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The development of a tumor-targeted immunotherapy is highly required. The most advanced application is the use of CD19 chimeric antigen receptor (CAR)T (CAR-T) cells to B cell malignancies, but there are still side effects including potential carcinogenicity of lentiviral or retroviral insertion into the host cell genome. Here, we developed a nonviral aptamer-T cell targeted strategy for tumor therapy. Tumor cells surface-specific ssDNA aptamers were conjugated to CD3 + T cells (aptamer-T cells) using N-azidomannosamine (ManNAz) sugar metabolic cell labeling and click chemistry. We found that the aptamer-T cells could specifically target and bind to tumor cells (such as SGC-7901 gastric cancer cell and CT26 colon carcinoma cell) in vitro and in mice after adoptively transfer in. Aptamer-T cells led to significant regression in tumor volume due to being enriched at tumor microenvironment and producing strong cytotoxicity activities of CD3 + T cells with enhanced perforin, granzyme B, CD107a, CD69, and FasL expression. Moreover, aptamer-T displayed even stronger antitumor effects than an anti-PD1 immune-checkpoint monoclonal antibody (mAb) treatment in mice and combination with anti-PD1 yielded synergic antitumor effects. This study uncovers the strong potential of the adoptive nonviral aptamer-T cell strategy as a feasible and efficacious approach for tumortargeted immunotherapy application.

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Section: Discussionmentioning

confidence: 99%

Aptamer-T Cell Targeted Therapy for Tumor Treatment Using Sugar Metabolism and Click Chemistry

et al. 2020

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“…Out of the nearly 20 papers published in the examined time frame, 14 were interested in the use of prostate specific antigen (PSA) as a biomarker (Table ). ,− Prostate cancer is one of the most commonly occurring cancers in the male population . The diagnostic threshold of PSA in serum, which is secreted by prostatic epithelial cells, is 4–10 ng/mL. , …”

Section: Aptamersmentioning

confidence: 99%

Biosensors Made of Synthetic Functional Nucleic Acids Toward Better Human Health

et al. 2019

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“…Thus, the application of powerful amplification strategies will greatly enhance detection ability. In recent years, several representative strategies have been used in micro-RNA analysis, such as enzyme-mediated amplification, rolling circle amplification (RCA), strand displacement amplification (SDA), enzyme-assisted target recycling (EATR), terminal deoxynucleotidyl transferase (TdT)-assisted amplification, and non-enzyme amplification including catalyzed-hairpin assembly amplification (CHA) and hybridization chain reaction (HCR), [20][21][22][23][24][25][26]. Among these, SDA and TdT-assisted amplification have gained attention owing to their merits of high amplification efficiency.…”

Section: Introductionmentioning

confidence: 99%

Label-Free miRNA-21 Analysis Based on Strand Displacement and Terminal Deoxynucleotidyl Transferase-Assisted Amplification Strategy

et al. 2022

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MicroRNAs (miRNAs) are regarded as a rising star in the biomedical industry. By monitoring slight increases in miRNA-21 levels, the possibilities of multi-type malignancy can be evaluated more precisely and earlier. However, the inconvenience and insensitivity of traditional methods for detecting miRNA-21 levels remains challenging. In this study, a partially complementary cDNA probe was designed to detect miRNA-21 with target-triggered dual amplification based on strand displacement amplification (SDA) and terminal deoxynucleotidyl transferase (TdT)-assisted amplification. In this system, the presence of miRNA-21 can hybridize with template DNA to initiate SDA, generating a large number of trigger molecules. With the assistance of TdT and dGTP, the released trigger DNA with 3′-OH terminal can be elongated to a superlong poly(guanine) sequence, and a notable fluorescence signal was observed in the presence of thioflavin T. By means of dual amplification strategy, the sensing platform showed a good response tomiRNA-21 with a detection limit of 1.7 pM (S/N = 3). Moreover, the specificity of this method was verified using a set of miRNA with sequence homologous to miRNA-21. In order to further explore its practical application capabilities, the expression of miRNA in different cell lines was quantitatively analyzed and compared with the qRT-PCR. The considerable results of this study suggest great potential for the application of the proposed approach in clinical diagnosis.

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A label-free fluorescence method based on terminal deoxynucleotidyl transferase and thioflavin T for detecting prostate-specific antigen

Cited by 22 publications

References 43 publications

Aptamer-T Cell Targeted Therapy for Tumor Treatment Using Sugar Metabolism and Click Chemistry

Aptamer-T Cell Targeted Therapy for Tumor Treatment Using Sugar Metabolism and Click Chemistry

Biosensors Made of Synthetic Functional Nucleic Acids Toward Better Human Health

Label-Free miRNA-21 Analysis Based on Strand Displacement and Terminal Deoxynucleotidyl Transferase-Assisted Amplification Strategy

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