A Label-Free Immunoassay Based Upon Localized Surface Plasmon Resonance of Gold Nanorods

Mayer, Kathryn M.; Lee, Seung–Hyun; Liao, Hongwei; Rostro, Betty C.; Fuentes, Amaris; Scully, Peter T.; Nehl, Colleen L.; Hafner, Jason H.

doi:10.1021/nn7003734

Cited by 423 publications

(399 citation statements)

References 44 publications

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“…The fluorescent intensity of hemoglobin coupling to the longitudinal plasmon resonance could be increased due to the longitudinal field enhancement of GNRs 20. The theory of longitudinal field was well developed and believed to be effective in describing the linear or nonlinear optical relationships from rough metal surface 21.…”

Section: Resultsmentioning

confidence: 99%

Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

et al. 2017

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Gold nanorods (GNRs) are a unique class of metal nanostructures that have attractive potentials in biomedical applications, and the concern on their biological safety is concomitantly increasing. Hemocompatibility is extremely important as their contact with blood circulation is unavoidable during in vivo delivery. Herein, two kinds of GNRs coated with hexadecyltrimethylammonium bromide (C‐GNRs) or poly(sodium‐p‐styrenesulfonate) are used to test their potential toxicological effects in blood. C‐GNRs with positive surface charges efficiently induce hemolysis when encountering erythrocytes. Cellular internalization of C‐GNRs is found, and they subsequently bind with hemoglobin, forming bioconjugates. The interaction between hemoglobin and C‐GNR (stoichiometry 32.7:1) is regulated by electrostatic forces. Chromophores like tryptophan (Trp) are found to interact with C‐GNRs, causing enhancement in fluorescence intensity. The conformation of protein is partially altered, evidenced by decrease in α‐helical, increase in β‐sheet and random coil of hemoglobin. Although C‐GNRs do not essentially decrease oxygen binding capacity of hemoglobin, they hamper oxygen release from the protein. Heme, the oxygen binding unit, releases from hemoglobin upon C‐GNR treatment, which could contribute to C‐GNR‐induced hemolysis. This study demonstrates the hematological effects of GNRs, revealing their potential risk in biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

et al. 2017

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“…The plasmonic properties of gold nanorods allow them to be used in photothermal therapy [1,2], imaging techniques [3,4], theranostics [5,6], sensing applications [7][8][9][10] and optical memories [11,12].…”

Section: Introductionmentioning

confidence: 99%

Thermal stability of mesoporous silica-coated gold nanorods with different aspect ratios

Gergely-Fülöp

Zámbó

Deák

2014

Materials Chemistry and Physics

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The effect of different temperatures (up to 900 °C) on the morphology of mesoporous silicacoated gold nanorods was systematically investigated. Gold nanorods with different aspect ratios (AR ranging from 2.5 to 4.3) were coated with a 15 nm thick mesoporous silica shell.Silicon supported monolayers of the particles were annealed in the temperature range of 300-900 °C. The resulting changes in particle morphology were investigated using scanning electron microscopy and visible wavelength extinction spectroscopy. The silica coating generally improved the stability of the nanorods from ca. 250 °C by several hundreds degree Celsius. For nanorods with AR<3 the shape and the aspect ratio change is only moderate up to 700 °C. At 900 °C these nanorods became spherical. For nanorods with AR>3, lower stability was found as the aspect ratio decrease was more significant and they transformed into spherical particles already at 700 °C. It was confirmed by investigating empty silica shells that the observed conformal change of the shell material when annealing core/shell particles is dictated by the deformation of the core particle. This also implies that a significant mechanical stress is exerted on the shell upon core deformation. In accordance with this, for the highest aspect ratio (AR~4) nanorod the shell breaks up at 900 °C and the gold cores were partially released and coalesced into large spherical particles.

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“…Among various synthesis methods, plant-mediated synthesis is preferred due to its cost effectiveness and environment-friendly nature [3,4]. GNP have applications in therapeutic [5], catalysis [2], immunoassay and immunosensing [6], diagnostic and drug delivery [7][8][9][10], analyte sensing [11][12][13], cancer cell diagnostics and selective photothermal therapy [14,15], photoacoustic imaging [16,17] and cancer treatment [18]. Various plant extracts have been explored for the synthesis of GNP, namely tamarind [19], Aloe vera [20], Pelargonium graveolens [21], Diopyros kaki [22], Eucalyptus camaldulensis and Pelargonium roseum [23], coriander [24], Scutellaria barbata [25], lemongrass [26], Datura metel, Carica papaya and Solanum melongena [27].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of gold nanoparticles synthesised by leaf and seed extract ofSyzygium cuminiL.

Kumar

Yadav

2012

Journal of Experimental Nanoscience

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Here, Syzygium cumini leaf extract (LE) and seed extract (SE) were explored for the synthesis of gold nanoparticles (GNP). LE and SE as well as their polar (water) fractions showed potential for GNP synthesis. Comparative synthesis kinetics and morphological characterisation studies revealed the synthesis of smaller sized GNP by LE than SE. Only polar (water) fractions showed potential for GNP synthesis, which are smaller in size compared to their respective extracts. SE contained more polyphenols and biochemical constituents than LE and therefore, showed higher synthesis rate and bigger sized GNP. Atomic force microscope and scanning electron microscope analysis indicated that both extracts and their fractions catalysed the synthesis of spherical GNP. The average size of GNP synthesised by LE, leaf water fraction (LWF), SE and seed water fraction (SWF) were 24, 23, 35 and 32 nm, respectively. Fourier transform infrared analysis identified the biomolecules involved in the synthesis and stability of GNP. This study documented the potential of S. cumini for the synthesis of GNP in addition to silver nanoparticles (SNP). However, nature and types of polyphenols involved in GNP synthesis seem to be different from that involved in SNP synthesis. This might be the possible reason for smaller sized GNP that SNP.

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A Label-Free Immunoassay Based Upon Localized Surface Plasmon Resonance of Gold Nanorods

Cited by 423 publications

References 44 publications

Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes

Thermal stability of mesoporous silica-coated gold nanorods with different aspect ratios

Characterisation of gold nanoparticles synthesised by leaf and seed extract ofSyzygium cuminiL.

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