A label-free luminescent light switching system for miRNA detection based on two color quantum dots

Borghei, Yasaman Sadat; Hosseini, Morteza; Ganjali, Mohammad Reza

doi:10.1016/j.jphotochem.2019.112351

Cited by 11 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After target recognition by green-QDs, orange-QDs were added and did not shift to "off-state" but their fluorescence appeared to increase as a result of a short distance allowing the fluorescence resonance energy transfer (FRET). The detection limit achieved using this strategy was of 14.0 pM [152].…”

Section: Quantum Dotsmentioning

confidence: 91%

See 1 more Smart Citation

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

Martino

Tammaro

Misso

et al. 2023

IJMS

View full text Add to dashboard Cite

MicroRNA (miRNA) are constituted of approximately 22 nucleotides and play an important role in the regulation of many physiological functions and diseases. In the last 10 years, an increasing interest has been recorded in studying the expression profile of miRNAs in cancer. Real time-quantitative polymerase chain reaction (RT-qPCR), microarrays, and small RNA sequencing represent the gold standard techniques used in the last 30 years as detection methods. The advent of nanotechnology has allowed the fabrication of nanostructured biosensors which are widely exploited in the diagnostic field. Nanostructured biosensors offer many advantages: (i) their small size allows the construction of portable, wearable, and low-cost products; (ii) the large surface–volume ratio enables the loading of a great number of biorecognition elements (e.g., probes, receptors); and (iii) direct contact of the recognition element with the analyte increases the sensitivity and specificity inducing low limits of detection (LOD). In this review, the role of nanostructured biosensors in miRNA detection is explored, focusing on electrochemical and optical sensing. In particular, four types of nanomaterials (metallic nanoparticles, graphene oxide, quantum dots, and nanostructured polymers) are reported for both detection strategies with the aim to show their distinct properties and applications.

show abstract

Section: Quantum Dotsmentioning

confidence: 91%

“…2023, 24, x FOR PEER REVIEW 27 of 36 not shift to "off-state" but their fluorescence appeared to increase as a result of a short distance allowing the fluorescence resonance energy transfer (FRET). The detection limit achieved using this strategy was of 14.0 pM [152]. Adapted with permission from Ref.…”

Section: Quantum Dotsmentioning

confidence: 99%

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

Martino

Tammaro

Misso

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Later, in 2020, Borghei and Hosseini developed a dual-emission ratiometric fluorescence sensor, based on QDs, for miRNA-155 detection. 138 The reported sensor comprises water-soluble green-emitting (525 nm) and orange-emitting CdTe QDs (599 nm), capped with mercaptoacetic acid (MAA or TGA).…”

Section: Sensing Of Cancer Mirnas Biomarkersmentioning

confidence: 99%

Quantum Dots for Cancer-Related miRNA Monitoring

2022

View full text Add to dashboard Cite

Quantum dots (QDs) possess exceptional optoelectronic properties that enable their use in the most diverse applications, namely, in the medical field. The prevalence of cancer has increased and has been considered the major cause of death worldwide. Thus, there has been a great demand for new methodologies for diagnosing and monitoring cancer in cells to provide an earlier prognosis of the disease and contribute to the effectiveness of treatment. Several molecules in the human body can be considered relevant as cancer markers. Studies published over recent years have revealed that micro ribonucleic acids (miRNAs) play a crucial role in this pathology, since they are responsible for some physiological processes of the cell cycle and, most important, they are overexpressed in cancer cells. Thus, the analytical sensing of miRNA has gained importance to provide monitoring during cancer treatment, allowing the evaluation of the disease’s evolution. Recent methodologies based on nanochemistry use fluorescent quantum dots for sensing of the miRNA. Combining the unique characteristics of QDs, namely, their fluorescence capacity, and the fact that miRNA presents an aberrant expression in cancer cells, the researchers created diverse strategies for miRNA monitoring. This review aims to present an overview of the recent use of QDs as biosensors in miRNA detection, also highlighting some tutorial descriptions of the synthesis methods of QDs, possible surface modification, and functionalization approaches.

show abstract

“…On the other hand, organic dyes [ 17 , 18 , 19 ] and nanoparticles [ 20 , 21 , 22 ] have been frequently applied as quenchers in the reported fluorescence sensors, while applications of QDs are relatively rare [ 23 , 24 , 25 ]. QDs show several advantages over the above materials, including facile synthesis, high resistance to photo-bleaching, large absorption cross-section, which designate them as promising quenchers in fluorescence analysis.…”

Section: Introductionmentioning

confidence: 99%

A Rapid, Fluorescence Switch-On Biosensor for Early Diagnosis of Sorghum Mosaic Virus

et al. 2022

View full text Add to dashboard Cite

For the first time, a nanobiosensor was established for Sorghum mosaic virus (SrMV) detection. The biosensor consists of cadmium telluride quantum dots (CdTe QDs) conjugated to the specific antibody (Ab) against SrMV coat protein (CP) and carbon quantum dots (C QDs) labeled with SrMV coat protein. The formation of the fluorophore-quencher immunocomplex CdTe QDs-Ab+C QDs-CP led to a distinct decrease in the fluorescence intensity of CdTe QDs. Conversely, the emission intensity of CdTe QDs recovered upon the introduction of unlabeled CP. The developed biosensor showed a limit of detection of 44 nM in a linear range of 0.10–0.54 μM and exhibited the strongest fluorescence intensity (about 47,000 a.u.) at 552 nm. This strategy was applied to detect purified CP in plant sap successfully with a recovery rate between 93–103%. Moreover, the feasibility of the proposed method was further verified by the detection of field samples, and the results were consistent with an enzyme-linked immunosorbent assay (ELISA). Contrarily to ELISA, the proposed biosensor did not require excessive washing and incubation steps, thus the detection could be rapidly accomplished in a few minutes. The high sensitivity and short assay time of this designed biosensor demonstrated its potential application in situ and rapid detection. In addition, the fluorescence quenching of CdTe QDs was attributed to dynamic quenching according to the Stern-Volmer equation.

show abstract

A label-free luminescent light switching system for miRNA detection based on two color quantum dots

Cited by 11 publications

References 29 publications

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

microRNA Detection via Nanostructured Biochips for Early Cancer Diagnostics

Quantum Dots for Cancer-Related miRNA Monitoring

A Rapid, Fluorescence Switch-On Biosensor for Early Diagnosis of Sorghum Mosaic Virus

Contact Info

Product

Resources

About