A Label-free Resonance Rayleigh Scattering Sensor for Detection of Thrombin Based on Aptamer Recognizing

Yu, Ling; Fu, Xiao Bei; Li, Nian Bing; Luo, Hong Qun

doi:10.2116/analsci.17p498

Cited by 7 publications

(3 citation statements)

References 49 publications

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“…Resonance Rayleigh scattering (RRS), determined by using a commonly used fluorescence meter, has been applied to determine biomolecules and inorganic ions [81]. Metal NPs have RRS effects and the resonance scattering peak is correlated with the size of the NPs.…”

Section: Resonance Rayleigh Scatteringmentioning

confidence: 99%

Recent Advances in Optical Detection of Aminoglycosides

Zhou

Cao

2020

Applied Sciences

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Aminoglycosides (AGs) are broad-spectrum antibiotics used in both human infection and animal medicine. The overuse of AGs causes undesirable residues in food, leading to serious health problems due to food chain accumulation. In recent years, various methods have been developed to determine AGs in food. Among these methods, fluorescent (FL), colorimetric and chemiluminescent (CL) optical methods possess advantages such as their simple instrumentation, low cost, simple operation, feasibility of realizing visualization, and smartphone imaging. This mini-review summarizes optical assays for the detection of AGs in food developed in recent years. The detection principles for different categories are discussed. Then, the amplification techniques for the ultrasensitive detection of AGs are introduced. We also discuss multiplex methods for the simultaneous detection of AGs. Finally, the challenges and future prospects are discussed in the Conclusions and Perspectives section.

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Section: Resonance Rayleigh Scatteringmentioning

confidence: 99%

Recent Advances in Optical Detection of Aminoglycosides

Zhou

Cao

2020

Applied Sciences

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“…In vitro selected aptamers have been recently used as molecular recognition elements in place of antibodies in analytical sciences. [1][2][3] Ever since various in vitro selection techniques to obtain aptamers have been explored after phage display system was developed by Smith in 1985, 4 aptamers are increasingly becoming prevalent. In the phage display system and similar systems involving cells at any in vitro selection steps, the genetic diversity of displayed proteins/peptides is largely suppressed because the number of transformable DNA is far less than the measure of genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

Selection of Ovalbumin-specific Binding Peptides through Instant Translation in Ribosome Display Using E. coli Extract

et al. 2021

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In vitro selection has been widely used to generate molecular recognition elements in analytical sciences. Although reconstituted types of in vitro transcription and translation (IVTT) system, such as PURE system, are nowadays widely used for ribosome display and mRNA/cDNA display, utilizing E.coli extract tends to be avoided presumably because it contains unfavorable contaminants such as ribonuclease. Nevertheless, the initial speed of protein translation in E.coli extract is markedly faster than that of PURE system. Thus, we hypothesized that E.coli extract is more appropriate for instant translation in ribosome display than PURE system. Here, we first revisit the potency of E. coli extract for ribosome display by shortening the translation time and then applied the optimized condition to the selection of peptide aptamers for ovalbumin (OVA). The OVA-binding peptides selected using E.coli extract exhibited specific binding to OVA even in the presence of 50% serum. We conclude that instant translation in ribosome display using E.coli extract has the potential to be used to generate easy-to-use and economical molecular recognition elements in analytical sciences.

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“…Therefore, the RRS spectra can be utilized to speculate molecular structure, charge distribution, and state of combination. Recently, the RRS technique has been widely applied to sensing metal ions, 8,9 biological molecules, [10][11][12] hazardous pollutants, 13,14 drug molecules, [15][16][17] dyes, [18][19][20] and so on. [21][22][23] The iron(III) cation is one of trace metal elements that play an important role in environmental and biological systems.…”

Section: Introductionmentioning

confidence: 99%

Resonance Rayleigh Scattering as a Tool for Isoelectric Point Monitoring and Iron(III) Cation Determination

Liu

et al. 2019

ANAL. SCI.

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A resonance Rayleigh scattering (RRS) technique was utilized as a tool for isoelectric point monitoring and iron(III) cation determination. The spectral properties of some amphoteric molecules (proteins and a DNA sequence) were investigated using the RRS technique. When the pH values were kept at around their isoelectric points, especially high RRS signals could be obtained, which were much stronger than those at other pH values. By using the C30 DNA sequence as a probe, the iron(III) cation can be detected rapidly. After iron(III) was added to a C30 solution, a significantly decreased RRS signal was obtained. The sensing process can be finished within 10 min with a detection limit of 0.9 μM. Thus, a sensitive, selective, and label-free method was successfully developed for iron(III) detection.

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A Label-free Resonance Rayleigh Scattering Sensor for Detection of Thrombin Based on Aptamer Recognizing

Cited by 7 publications

References 49 publications

Recent Advances in Optical Detection of Aminoglycosides

Recent Advances in Optical Detection of Aminoglycosides

Selection of Ovalbumin-specific Binding Peptides through Instant Translation in Ribosome Display Using E. coli Extract

Resonance Rayleigh Scattering as a Tool for Isoelectric Point Monitoring and Iron(III) Cation Determination

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