A label-free voltammetric immunoassay based on 3D-structured rGO–MWCNT–Pd for detection of human immunoglobulin G

Liu, Lei; Li, Yueyuan; Tian, Lihui; Guo, Tian; Cao, Wei; Wei, Qin

doi:10.1016/j.snb.2015.01.069

Cited by 34 publications

(17 citation statements)

References 38 publications

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“…Analytical performances have been compared with the other reported immunosensors and tabulated in Table 1 1,49,53–58. By comparing with other immunosensors, it was observed that the anti‐IgG/PtNPs/PAAMI/LAG immunosensor exhibited a sufficient detection range and a lower LOD for recognizing IgG.…”

Section: Resultsmentioning

confidence: 99%

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

Barman

Zahed

Sharifuzzaman

et al. 2020

Adv Funct Materials

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Current immunosensors have an insufficient number of binding sites for the recognition of biomolecules, which leads to false positive or negative results. In this research, a facile, cost‐effective, disposable, and highly selective electrochemical immunosensing platform is developed based on cationic polyelectrolyte polyallylamine (PAAMI) anchored laser‐ablated graphene (LAG). Here, for the first time, PAAMI is introduced to stabilize LAG flakes, while retaining the intrinsic thermal and electronic properties of the substrate by noncovalent π–π interaction and electrostatic physical absorption. The sensing platform offers a suitable number of anchoring sites for the immobilized antibodies by providing NH2 functional groups. The proper grafting of PAAMI is confirmed through X‐ray photoelectron spectroscopy and Raman spectroscopy. The immunosensing platform is applied to detect immunoglobulin (IgG) biomarkers as a proof of concept. Under optimized conditions, the sensing platform exhibits a linear range of 0.012–15 and 15–352 ng mL−1 with a limit of detection of 6 pg mL−1 for IgG detection with high selectivity. Based on the analysis, the developed immunosensing platform can be used for point‐of‐care detection of IgG in clinical diagnostic centers. Furthermore, the developed strategy is well suited for the detection of other cancer biomarkers after immobilizing the relevant antibodies.

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Section: Resultsmentioning

confidence: 99%

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

Barman

Zahed

Sharifuzzaman

et al. 2020

Adv Funct Materials

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“…The sensor also offers low limit of detection of 0.15 ng mL À1 , limit of quantitation of 0.50 ng mL À1 and high sensitivity (11.297 lA cm À2 ), which are sufficient for the detection of immunoglobulin G at the physiological level. Table 1 shows the detection performances for detecting immunoglobulin G using our proposed immunosensor as compared to other immunosensors reported in the literatures [32][33][34][35][36][37][38][39]. Entries 1-4 are of sandwich structured immunosensors whilst entries 5-8 are of label-free immunosensors.…”

Section: Linear Response and Limit Detection Of The Immunosensormentioning

confidence: 99%

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

Putnin

Jumpathong

Laocharoensuk

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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This work focuses on fabricating poly(2-aminobenzylamine)-modified screen-printed carbon electrode as an electrochemical immunosensor for the label-free detection of human immunoglobulin G. To selectively detect immunoglobulin G, the anti-immunoglobulin G antibody with high affinity to immunoglobulin G was covalently linked with the amine group of poly(2-aminobenzylamine) film-deposited screen-printed carbon electrode. The selectivity for immunoglobulin G was subsequently assured by being challenged with redox-active interferences and adventitious adsorption did not significantly interfere the analyte signal. To obviate the use of costly secondary antibody, the [Fe(CN)] redox probe was instead applied to measure the number of human immunoglobulin G through the immunocomplex formation that is quantitatively related to the level of the differential pulse voltammetric current. The resulting immunosensor exhibited good sensitivity with the detection limit of 0.15 ng mL, limit of quantitation of 0.50 ng mL and the linear range from 1.0 to 50 ng mL. Given those striking analytical performances and the affordability arising from using cheap screen-printed carbon electrode with label-free detection, the immunosensor serves as a promising model for the next-step development of a diagnostic tool.

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“…Recently, label-free approaches have been proposed for the determination of IgG in human serum based on mass spectrometry [26], on magnetically assisted surface enhanced Raman spectroscopy through a Fe 3 O 4 @Ag@streptavidin@anti-IgG nanocomposite [27], on nanoporous hydrogel photonic crystal structures containing immobilized protein A [28], on attenuated total reflectance mid-infrared spectroscopy coupled to multivariate calibration [29], and on voltammetric detection of IgG binding to reduced graphene oxide@multiwalled carbon nanotube@palladium@anti-IgG nanoparticles [30]. Nevertheless, these methods still require non-portable equipment [26], cumbersome synthesis of materials [28,30], or advanced data processing [29].…”

Section: Introductionmentioning

confidence: 99%

Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum

et al. 2017

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Immunoglobulin G (IgG) represents the major fraction of antibodies in healthy adult human serum, and deviations from physiological levels are a generic marker of disease corresponding to different pathologies. Therefore, screening methods for IgG evaluation are a valuable aid to diagnostics. The present work proposes a rapid, automatic, and miniaturized method based on UV-vis micro-bead injection spectroscopy (μ-BIS) for the real-time determination of human serum IgG with label-free detection. Relying on attachment of IgG in rec-protein G immobilized in Sepharose 4B, a bioaffinity column is automatically assembled, where IgG is selectively retained and determined by on-column optical density measurement. A "dilution-and-shoot" approach (50 to 200 times) was implemented without further sample treatment because interferences were flushed out of the column upon sample loading, with minimization of carryover and cross-contamination by automatically discarding the sorbent (0.2 mg) after each determination. No interference from human serum albumin at 60 mg mL in undiluted sample was found. The method allowed IgG determination in the range 100-300 μg mL (corresponding to 5.0-60 mg mL in undiluted samples), with a detection limit of 33 μg mL (1.7 mg mL for samples, dilution factor of 50). RSD values were < 9.4 and < 11.7%, for intra and inter-assay precision, respectively, while recovery values for human serum spiked with IgG at high pathological levels were 97.8-101.4%. Comparison to commercial ELISA kit showed no significant difference for tested samples (n = 8). Moreover, time-to-result decreased from several hours to < 5 min and analysis cost decreased 10 times, showing the potential of the proposed approach as a point-of-care method. Graphical abstract Micro-Bead Injection Spectroscopy method for real time, automated and label-free determination of total serum human Immunoglobulin G (IgG). The method was designed for Lab-on-Valve (LOV) platforms using a miniaturised protein G bioaffinity separative approach. IgG are separated from serum matrix components upon quantification with low non-specific binding in less than 5 min.

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A label-free voltammetric immunoassay based on 3D-structured rGO–MWCNT–Pd for detection of human immunoglobulin G

Cited by 34 publications

References 38 publications

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G

Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum

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