A laboratory hive for frequent collection of honeybee eggs

Omholt, Stig W.; Hagen, Arne; Elmholdt, Odd; Rishovd, Svein

doi:10.1051/apido:19950404

Cited by 20 publications

(15 citation statements)

References 7 publications

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“…Egg collection hives were constructed as described by Omholt et al (1995). The design was modified so that a hive consisted of a single hive box with 2 aluminum frames of the same size as Norwegian standard frames (370 × 255 mm).…”

Section: Laboratory Hivesmentioning

confidence: 99%

“…This allowed us to collect and introduce eggs by extracting and inserting individual cell bases, as the bases were easily accessible from the side of the frames that made out the outer walls of the hive. For further information on the egg collection hive design, see Omholt et al (1995).…”

Section: Laboratory Hivesmentioning

confidence: 99%

“…The bees were offered sugar syrup (40%) and pollen from freestanding feeders. In addition, the colonies were provided with pollen dough through the crown boards of the hives (as described by Omholt et al, 1995).…”

Section: Laboratory Hivesmentioning

confidence: 99%

“…Techniques such as microinjection and enucleation require that honey bee eggs of known age are staged in a laboratory setting (Omholt et al, 1995), but this is challenging because the queen will only lay eggs in the milieu of the hive. Further, the eggs adhere to the bottom of wax cells, which makes them difficult to remove.…”

Section: Introductionmentioning

confidence: 99%

“…Further, the eggs adhere to the bottom of wax cells, which makes them difficult to remove. Eggs of known age may be obtained by caging a queen on a comb, but the disturbance caused by caging often interferes with the resumption of normal egg laying (Omholt et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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A new method for rearing genetically manipulated honey bee workers

et al. 2005

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-Advanced functional genomic research on the honey bee (Apis mellifera) will require methods that allow researchers to work with bees derived from genetically manipulated embryos. In vitro rearing of honey bees is laborious, and it is often difficult to obtain individuals that span a normal phenotypic range. We present a technique that allows manipulated honey bee eggs to be introduced into hives so the larvae can be reared in a colony setting. Newly laid eggs on removable cell bases were injected with nuclease free H 2 O, double-stranded RNA (dsRNA), or left untreated. They were inserted into specially designed hives where they hatched. Colonies accepted a satisfactory proportion of eggs from all treatment groups (28-53%). Further, a set of physiological and morphological traits (i.e., total protein in the hemolymph, head width, antennal length, and the length of a compound vein) were compared between workers derived from untreated, incubated eggs, and bees that naturally emerged in the hives. No significant differences were found between the groups. Our method therefore overcomes the challenges associated with in vitro rearing.Apis mellifera / rearing protocol / laboratory hive / functional genomic research

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Section: Laboratory Hivesmentioning

confidence: 99%

Section: Laboratory Hivesmentioning

confidence: 99%

Section: Laboratory Hivesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A new method for rearing genetically manipulated honey bee workers

et al. 2005

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Chimeric honeybees (Apis mellifera) produced by transplantation of embryonic cells into pre‐gastrula stage embryos and detection of chimerism by use of microsatellite markers

Bergem

Norberg

Røseth

et al. 2006

Molecular Reproduction Devel

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The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produce chimeric honeybees by use of cell transplantation. Embryonic cells were transplanted between pre-gastrula stage embryos (32-34 hr after oviposition) and hatched larvae were reared in vitro for 4 days. Chimeric individuals were detected by use of microsatellite analysis and a conservative estimation approach. 4.8% of embryos, posteriorly injected with embryonic cells, developed into chimeric honeybee larvae. By injection of cells pre-stained with fluorescent cell tracer dye, we studied the integration of transplanted cells in the developing embryos. Number of injected cells varied from 0 to 50 and cells remained and multiplied mainly in the area of injection.

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