A laminin‐211‐derived bioactive peptide promotes the osseointegration of a sandblasted, large‐grit, acid‐etched titanium implant

Abstract

Early implant loading is very important for reducing the duration of missing teeth in human patients. The laminin‐derived peptide, DLTIDDSYWYRI motif (Ln2‐P3), accelerates bone healing. Therefore, to investigate the hypothesis that Ln2‐P3 increases the bone response to sandblasted, large‐grit, acid‐etched (SLA) titanium implants, the effect of the Ln2‐P3 peptide on the osseointegration of SLA titanium implants was evaluated in vitro and in vivo. Human osteoblast‐like cells were cultured on untreated, scrambled peptide (SP)‐treated, and Ln2‐P3‐treated SLA titanium discs, and the cellular responses of these cells were evaluated. The Ln2‐P3 treatment augmented osteoblast attachment and spreading, alkaline phosphatase activity, and the expression of osteogenic marker genes. Furthermore, the untreated and Ln2‐P3‐treated SLA titanium implants were inserted into the tibiae of rabbits for 9 and 11 days. Compared with the untreated implants, the Ln2‐P3‐treated implants showed a significantly higher bone‐to‐implant contact ratio at Day 9 after implantation and an increased bone area. The Ln2‐P3 treatment of the SLA titanium implant surface augmented osteoblastic activity and accelerated peri‐implant bone formation at the bone–implant interface. Overall, these results indicated that compared with the SLA titanium surface alone, the Ln2‐P3 peptide‐treated SLA titanium surface enhances initial osseointegration, thereby facilitating earlier implant loading.

Osteoconductive and Osteoinductive Surface Modifications of Biomaterials for Bone Regeneration: A Concise Review

“…Yu et al [131] applied picosecond laser ablation to modify Ti substrate and demonstrated that the resultant surface of Ti with micro-groves promoted cell adhesion. The subtractive modifications of biomaterial surface may also be applied to prepare the surface before the coating process (Figure 4) [132][133][134][135][136][137][138][139]. By increasing the roughness of the surface, better adhesive bond between the coating and the surface of modified material may be achieved [140].…”

Effect of Hafnium Coating on Osseointegration of Titanium Implants: A Split Mouth Animal Study

“…In the current study, hafnium coating appears to have offered similar biocompatibility (aspartate transaminase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) enzyme assay), statistically significant improvement (independent Student's t-test, p < 0:05) in insertion torque (25:42 ± 3:96) and removal torque (29:17 ± 2:88) than commercially pure titanium with insertion torque (22:08 ± :57) and removal torque (25:42 ± 2:57). Hafnium has proved to have good tissue response and osseointegration, along with required mechanical properties [39][40][41][42][43][44][45][46][47][48]. Though these results seem to favour hafnium, it is necessary to analyse the factors that could have confounded our study.…”

“…In 1984, Marcel Pourbaix proposed hafnium as a metal to be considered for surgical implants due to the passive state of the metal with properties like high ductility, strength, resistance to corrosion, and mechanical damage. Various in vitro studies were conducted on hafnium metal [33][34][35][36][37][38][39][40][41][42].…”

Biochemical Modification of Titanium Oral Implants: Evidence from In Vivo Studies

“…More recently, Choi et al studied the influence on osseointegration of an active, laminin-derived peptide (Ln2-P3) in a rabbit tibia model [ 49 ]. Laminin is an ECM protein mainly involved in the mechanism of adhesion and proliferation of basal lamina cells [ 51 ].…”