A lanthanide-peptide-derived bacterium-like nanotheranostic with high tumor-targeting, -imaging and -killing properties

Abstract

Nanostructures formed with bioactive peptides offer an exciting prospect in clinical oncology as a novel class of therapeutic agents for human cancers. Despite their therapeutic potential, however, peptide-based nanomedicines are often inefficacious in vivo due to low cargo-loading efficiency, poor tumor cell-targeting specificity and limited drug accumulation in tumor tissues. Here, we describe the design, via assembly of a p53-activating peptide termed PMI, functionalized PEG and fluorescent lanthanide oxyfluoride nanocrystals, of a novel nanotheranostic shaped in flexible rods. This lanthanide-peptide nanorod or LProd of bionic nature exhibited significantly enhanced tumor-targeting and -imaging properties compared to its spherical counterpart. Importantly, LProd potently inhibited tumor growth in a mouse model of human colon cancer through activating tumor suppressor protein p53 via MDM2/MDMX antagonism, while maintaining a highly favorable biosafety profile. Our data demonstrate that LProd as a multifunctional theranostic platform is ideally suited for tumor-specific peptide drug delivery with real-time disease tracking, thereby broadly impacting clinical development of antitumor peptides.

A general-purpose Nanohybrid fabricated by Polymeric Au(I)-peptide precursor to wake the function of Peptide Therapeutics

“…Linear peptides are conformationally disordered in aqueous solutions under their own steam and, consequently, tendentious to proteolysis. Conjugation of peptides with nanoparticles would improve steric hindrance against peptidase, resulting in the resistance to proteolysis 5 , 44 . We comparatively quantified the susceptibility of Pep-AuNPs and Pep-Au SNH to chymotrypsin using HPLC (Figure 3 E-G).…”

A capillary electrophoresis strategy to sensitively detect dynamic properties of coiled coil polypeptides

“…PC 10 P‐FITC, PC 10 ‐RBITC, and AC 10 ‐RBITC were prepared through the published procedures [24–26]. Briefly, 3 mg of polypeptide was dissolved in 1 mL carbonate‐bicarbonate buffer solution (0.1 M, pH 9), and 100 μL 1 mg/mL of FITC or RBITC was added dropwise.…”

Current update on nanoplatforms as therapeutic and diagnostic tools: A review for the materials used as nanotheranostics and imaging modalities

“…In vivo studies involving a mouse model with human colon cancer showed that the nanosystem is efficacious. More specifically, the nanorods can inhibit tumor growth and be easily mapped [211] . You et al prepared multifunctional nanosystems with sufficient cellular uptake, specific target and controlled release efficacy.…”