A Large Animal Survival Model to Evaluate Bariatric Surgery Mechanisms

Simianu, Vlad V.; Sham, Jonathan G.; Wright, Andrew S.; Stewart, S; Alloosh, Mouhamad; Sturek, Michael; Cummings, David E.; Flum, David R.

doi:10.4236/ss.2015.68050

Cited by 2 publications

(2 citation statements)

References 36 publications

(44 reference statements)

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“…The rodents were allowed to acclimatize for 7 days to laboratory conditions before any experiment. The rodents' use and care were in strict compliance with the National Research Council (NRC) Guidelines on the Care and Use of Laboratory Animals [21].…”

Section: Animalsmentioning

confidence: 99%

Effect of Extracts of Kigelia Africana Fruit and Sorghum Bicolor Stalk on the Biochemical Parameters of Alloxan-Induced Diabetic Rats

Obianagha

Okafor

Chukwuani

et al. 2021

JPRI

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Diabetes and its complication continue to remain a concern in the world population despite the introduction of various hypoglycemic agents. Biochemical changes remain the major observable, clinical, and pathological factors as a derangement in the levels of these parameters increase the risk of developing complications. Although herbal extracts for the treatment of diabetes have been scientifically validated, the scientific interaction and alteration of the biochemical parameters due to the administration of this herbal product are not thoroughly investigated in this region. Therefore, this study aims to determine the effect of the extract of Kigeliaafricana and Sorghum bicolor stalk on the biochemical parameters of diabetic-induced rats. A total of eleven groups containing five rats per group of randomly selected Alloxan-induced Diabetes Healthy Wistar rats of both sexes were used in the study, with one serving as the control group, another serving as glibenclamide treated group, and the remaining nine, extract treated groups. After the overnight fast, the control group received a dose of 0.5 ml of 2% w/v acacia solution. Glibenclamide treated group received 600 μg/kg body weight (bwt) glibenclamide. In contrast, the other nine groups received specified doses (125, 250, and 500mg/kg bwt) of Kigeliaafricana and Sorghum bicolor extracts singly and in a mixture of ratio 1:1, respectively. After receiving the specified doses once a day orally for 30 days, the rats fasted overnight, and 5 ml of blood collected via cardiac puncture into heparinized and fluoride bottles. The samples were spun and separated for biochemical profiles (plasma glucose, lipid profile, liver function test, and electrolytes urea and creatinine) using a commercially prepared kit with outlined procedures. The result showed a significant decrease (p < 0.05) of plasma glucose level in the extract-treated rats, with a remarkable increase in untreated diabetic rats compared to the control. A significant reduction (p < 0.01) in the plasma levels of triglyceride and LDL-Chol was also observed in all treated groups at various doses of extracts compared to control with HDL-Chol values marginally increased in all extract-treated groups. Urea and creatinine showed a significant decrease (p < 0.01) in all treated doses except the lowest when compared to the control, while all the electrolytes parameters (Na+, K+, Cl- and HCO3-) show no significant difference (p > 0.05) across the groups when compared to the control. Liver function showed no significant differences in all parametersexcept for the noticeable decreasing effects on AST and ALT values compared to the control. The extracts and their mixture exhibited antidiabetic and hypolipidaemic activities and cardiovascular benefits due to their considerable lowering effects on total cholesterol, LDL-Chol, triglycerides, and increase in the HDL-Chol levels. The observed biological actions may be due to the presence of different phytocomponents present in the plant extracts. There is, therefore, a need to determine which of the active constituents has the main antidiabetic and hypolipidaemic effect for their optimal usage.

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Section: Animalsmentioning

confidence: 99%

Effect of Extracts of Kigelia Africana Fruit and Sorghum Bicolor Stalk on the Biochemical Parameters of Alloxan-Induced Diabetic Rats

Obianagha

Okafor

Chukwuani

et al. 2021

JPRI

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“…Porcine models have more translational value than rat models, even if rats are more often employed as preclinical models of metabolic surgery. 16 , 17 , 18 , 19 Pigs are in fact omnivorous and are similar to humans concerning the morphology and the physiology of their gastrointestinal tract, pancreas, propension to obesity and sedentary, metabolic biomarker levels and drug pharmacokinetics, 20 , 21 making it a particularly suitable preclinical model for these kinds of studies.…”

Section: Introductionmentioning

confidence: 99%

Effects of subtotal pancreatectomy and long‐term glucose and lipid overload on insulin secretion and glucose homeostasis in minipigs

Goutchtat

Quenon

Clarisse

et al. 2023

Endocrino Diabet & Metabol

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Introduction Nowadays, there are no strong diabetic pig models, yet they are required for various types of diabetes research. Using cutting‐edge techniques, we attempted to develop a type 2 diabetic minipig model in this study by combining a partial pancreatectomy (Px) with an energetic overload administered either orally or parenterally. Methods Different groups of minipigs, including Göttingen‐like (GL, n = 17) and Ossabaw (O, n = 4), were developed. Prior to and following each intervention, metabolic assessments were conducted. First, the metabolic responses of the Göttingen‐like (n = 3) and Ossabaw (n = 4) strains to a 2‐month High‐Fat, High‐Sucrose diet (HFHSD) were compared. Then, other groups of GL minipigs were established: with a single Px (n = 10), a Px combined with a 2‐month HFHSD (n = 6), and long‐term intraportal glucose and lipid infusions that were either preceded by a Px (n = 4) or not (n = 4). Results After the 2‐month HFHSD, there was no discernible change between the GL and O minipigs. The pancreatectomized group in GL minipigs showed a significantly lower Acute Insulin Response (AIR) (18.3 ± 10.0 IU/mL after Px vs. 34.9 ± 13.7 IU/mL before, p < .0005). In both long‐term intraportal infusion groups, an increase in the Insulinogenic (IGI) and Hepatic Insulin Resistance Indexes (HIRI) was found with a decrease in the AIR, especially in the pancreatectomized group (IGI: 4.2 ± 1.9 after vs. 1.5 ± 0.8 before, p < .05; HIRI (×10−5): 12.6 ± 7.9 after vs. 3.8 ± 4.3 before, p < .05; AIR: 24.4 ± 13.7 µIU/mL after vs. 43.9 ± 14.5 µIU/mL before, p < .005). Regardless of the group, there was no fasting hyperglycemia. Conclusions In this study, we used pancreatectomy followed by long‐term intraportal glucose and lipid infusions to develop an original minipig model with metabolic syndrome and early signs of glucose intolerance. We reaffirm the pig's usefulness as a preclinical model for the metabolic syndrome but without the fasting hyperglycemia that characterizes diabetes mellitus.

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A Large Animal Survival Model to Evaluate Bariatric Surgery Mechanisms

Cited by 2 publications

References 36 publications

Effect of Extracts of Kigelia Africana Fruit and Sorghum Bicolor Stalk on the Biochemical Parameters of Alloxan-Induced Diabetic Rats

Effect of Extracts of Kigelia Africana Fruit and Sorghum Bicolor Stalk on the Biochemical Parameters of Alloxan-Induced Diabetic Rats

Effects of subtotal pancreatectomy and long‐term glucose and lipid overload on insulin secretion and glucose homeostasis in minipigs

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