A large population of diverse neurons in the Drosophilacentral nervous system expresses short neuropeptide F, suggesting multiple distributed peptide functions

Nässel, Dick R.; Enell, Lina E.; Santos, Jonathan G; Wegener, Christian; Johard, Helena A.D.

doi:10.1186/1471-2202-9-90

Cited by 157 publications

(192 citation statements)

References 86 publications

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“…Three PCs are found in D. melanogaster [18]. PCs sometimes cleave a monobasic amino acid, which suggests that sNPF2 may be cleaved further and produce sNPF2 [1][2][3][4][5][6][7][8][9][10] (WFGDVNQKPI) and sNPF2 [12][13][14][15][16][17][18][19] (SPSLRLRFa) found in the larval and adult tissues. sNPF2 1-10 is a biologically inactive peptide confirmed in the Western blot analysis with Drosophila neuronal cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In the mammalian CHO cell based bioluminescence assay for sNPFR1, sNPF1, sNPF2 [12][13][14][15][16][17][18][19] , sNPF3, and sNPF4 peptides show high calcium responses [12]. However, in the Xenopus oocytes electrophysiological assay for sNPFR1, sNPF1, sNPF2, and sNPF2 [9][10][11][12][13][14][15][16][17][18][19] peptides show higher inward chloride current responses than sNPF3 or sNPF4 [13].…”

Section: Introductionmentioning

confidence: 99%

“…sNPFp antibody was raised against DPSLPQMRRTAYDDLLEREL of the sNPF precursor (green color in Fig. 2A) in rabbits [16,17] and sNPF1 antibody was raised against the amidated sNPF1 (AQRSPSLRLRFa) in guinea pigs.…”

Section: Immunostainingmentioning

confidence: 99%

“…However, in the Xenopus oocytes electrophysiological assay for sNPFR1, sNPF1, sNPF2, and sNPF2 [9][10][11][12][13][14][15][16][17][18][19] peptides show higher inward chloride current responses than sNPF3 or sNPF4 [13]. In the mammalian CHO-K1 cell based radioreceptor assay, sNPF1, sNPF2, and sNPF2 [9][10][11][12][13][14][15][16][17][18][19] peptides are more potent than sNPF2 [12][13][14][15][16][17][18][19] , sNPF3 or sNPF4 [10] (Supplementary Table S1). Since the sNPF peptides show different biological activities for sNPFR1 in vitro and heterologous assays and its functions are unknown, we examined whether sNPF peptides regulate growth through the ERK-insulin pathway.…”

Section: Introductionmentioning

confidence: 99%

“…2A). However, in previous mass spectrometry studies using larval and adult tissues, AQRSPSLRLRFa (sNPF1), SPSLRLRFa (sNPF2 [12][13][14][15][16][17][18][19] ), SDPDMLNSIVE (sNPF-AP-1), WFGDVNQKPI (sNPF2 [1][2][3][4][5][6][7][8][9][10] ), SPSLRLRFa (sNPF2 [12][13][14][15][16][17][18][19] ) peptides are found [7][8][9]. PQRLRWa (sNPF3) and PMRLRWa (sNPF4) are also identified in adult body extract [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Processed short neuropeptide F peptides regulate growth through the ERK‐insulin pathway in Drosophila melanogaster

et al. 2009

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a b s t r a c tThe Drosophila sNPF gene regulates growth through the ERK-insulin pathway. sNPF encodes a precursor protein that is processed and produces biologically active sNPF peptides. However, the functions of these peptides are not known. In Drosophila neuronal cells in culture and in flies in vivo, sNPF1 and sNPF2 activated the ERK-insulin pathway and regulated body growth. In addition, the sNPF precursor and the processed sNPF peptide were co-localized in the neurons of the central nervous system. These results indicate that sNPF1 and sNPF2 peptides processed from the sNPF precursor are sufficient for regulating body growth through the ERK-insulin pathway in Drosophila.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Immunostainingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Processed short neuropeptide F peptides regulate growth through the ERK‐insulin pathway in Drosophila melanogaster

et al. 2009

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A cyclic nucleotide‐gated channel in the brain regulates olfactory modulation through neuropeptide F in fruit fly Drosophila melanogaster

Lee

Jones

Kim

2019

Arch Insect Biochem Physiol

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Olfactory sensing and its modulation are important for the insects in recognizing diverse odors from the environment and in making correct decisions to survive. Identifying new genes involved in olfactory modulation and unveiling their mechanisms may lead us to understand decision making processes in the central nervous system. Here, we report a novel olfactory function of the cyclic nucleotide-gated (CNG) channel CG42260 in modulating ab3A olfactory sensory neurons, which specifically respond to food-derived odors in fruit fly Drosophila melanogaster. We found that two independent CG42260 mutants show reduced responses in the ab3A neurons. Unlike mammalian CNGs, CG42260 is not expressed in the odorant sensory neurons but broadly in the central nervous system including neuropeptide-producing cells. By using molecular genetic tools, we identified CG42260 expression in one pair of neuropeptide F (NPF) positive L1-l cells known to modulate food odor responsiveness. Knockdown of CG42260 in the NPF neurons reduced production of NPF in Ll-1 cells, which in turn, led to reduction of neuronal responses of the ab3A neurons. Our findings show the novel biological function of CG42260 in modulating olfactory responses to food odor through NPF. K E Y W O R D S

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Peptidergic clock neurons in Drosophila: Ion transport peptide and short neuropeptide F in subsets of dorsal and ventral lateral neurons

Johard

Yoishii

Dircksen

et al. 2009

J of Comparative Neurology

196

209

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About 150 clock neurons are clustered in different groups in the brain of Drosophila. Among these clock neurons, some pigment-dispersing factor (PDF)-positive and PDF-negative lateral neurons (LNs) are principal oscillators responsible for bouts of activity in the morning and evening, respectively. The full complement of neurotransmitters in these morning and evening oscillators is not known. By using a screen for candidate neuromediators in clock neurons, we discovered ion transport peptide (ITP) and short neuropeptide F (sNPF) as novel neuropeptides in subpopulations of dorsal (LN(d)s) and ventral (s-LN(v)s) LNs. Among the six LN(d)s, ITP was found in one that coexpresses long neuropeptide F (NPF) and cryptochrome. We detected sNPF in two LN(d)s that also express cryptochrome; these cells are distinct from three LN(d)s expressing NPF. Thus, we have identified neuropeptides in five of the six LN(d)s. The three LN(d)s expressing cryptochrome, with either ITP or sNPF, are the only ones with additional projections to the accessory medulla. Among the five s-LN(v)s in the adult brain, ITP was detected in the fifth neuron that is devoid of PDF and sNPF in the four neurons that also express PDF. By using a choline acetyltransferase (Cha) Gal4, we detected Cha expression in the two sNPF producing LN(d)s and in the fifth s-LN(v). In the larval brain, two of the four PDF-producing s-LN(v)s coexpress sNPF. Our findings emphasize that the LN(d)s are heterogeneous both anatomically and with respect to content of neuropeptides, cryptochrome, and other markers and suggest diverse functions of these neurons.

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A large population of diverse neurons in the Drosophilacentral nervous system expresses short neuropeptide F, suggesting multiple distributed peptide functions

Cited by 157 publications

References 86 publications

Processed short neuropeptide F peptides regulate growth through the ERK‐insulin pathway in Drosophila melanogaster

Processed short neuropeptide F peptides regulate growth through the ERK‐insulin pathway in Drosophila melanogaster

A cyclic nucleotide‐gated channel in the brain regulates olfactory modulation through neuropeptide F in fruit fly Drosophila melanogaster

Peptidergic clock neurons in Drosophila: Ion transport peptide and short neuropeptide F in subsets of dorsal and ventral lateral neurons

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