A large-scale viability assessment of the potato cryobank at the International Potato Center (CIP)

Vollmer, Rainer; Villagaray, Rosalva; Cárdenas, José; Castro, Mario; Chávez, Oswaldo; Anglin, Noelle L.; Ellis, David

doi:10.1007/s11627-017-9846-1

Cited by 29 publications

(18 citation statements)

References 12 publications

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“…Finally, different response variables were used for the assessment in the reviewed publications. Some authors reported complete plant recovery (Schäfer-Menuhr et al 1997;Vollmer et al 2017), while others reported only survival (swollen shoot tips of > 3 mm) or shoot formation (Kim et al 2006;Panta et al 2015).…”

Section: Resultsmentioning

confidence: 99%

“…RS was supplemented with five different sucrose concentrations: 0.0 M, 0.3 M, 0.6 M, 0.9 M, and 1.2 M (control treatment). The RS sucrose concentration of 1.2 M was routinely used at CIP for more than 10 years (Panta et al 2015;Vollmer et al 2017). Shoot tips were rewarmed in rewarming solution (RS) for 20 min at room temperature (20-24 °C) under ambient light conditions (20-30 µmol m −2 s −1 ).…”

Section: Rewarmingmentioning

confidence: 99%

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Cryopreserved potato shoot tips showed genotype-specific response to sucrose concentration in rewarming solution (RS)

Vollmer

Villagaray

Castro

et al. 2018

Plant Cell Tiss Organ Cult

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Cryopreservation protocols have been successfully developed for hundreds of species and thousands of genotypes in laboratories around the world. In many of the protocols, the rewarming process occurs in a rewarming solution (RS) with a high concentration of sucrose (0.8-1.2 M). Warming rate and associated conditions influence cell rehydration, loss of accumulated solutes (e.g. cryoprotectants) and recrystallization of small ice crystals in the nuclei. The need for, and effect of, high sucrose concentrations in the RS post-thawing regrowth rate after liquid nitrogen exposure was assessed in the range of 0.0-1.2 M sucrose with a set of 16 potato landraces cryopreserved with the PVS2-droplet vitrification method. The results showed no significant difference for the average recovery rate (81-87%) between sucrose concentrations of 0.3 M to 1.2 M. Fourteen of 16 accessions had their highest recovery rate with sucrose concentrations between 0.3 and 0.9 M. The experimental results were subsequently extended to a genetically diverse set of 85 potato accessions (nine taxa), which demonstrated significantly higher recovery rates of 55-61% with RS sucrose concentrations of 0.3-0.9 M, compared to the sucrose concentrations of 0.0 M (37%) and 1.2 M (44%). Only one of 85 accessions showed its highest recovery rate with the routinely used RS sucrose concentration of 1.2 M. Of all the concentrations tested, 0.6 M sucrose appeared to be the best bet in terms of recovery rates across the genotypes; therefore, our routine protocol has been changed from 1.2 M sucrose to 0.6 M. The specific response to low (0.0 M) and high RS sucrose concentrations (1.2 M) was highly variable within species/subspecies and appears to be genotype specific. Thus, caution should be taken in generalizing experimental cryopreservation results obtained with a limited number of accessions to larger germplasm collections.

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Section: Resultsmentioning

confidence: 99%

Section: Rewarmingmentioning

confidence: 99%

Cryopreserved potato shoot tips showed genotype-specific response to sucrose concentration in rewarming solution (RS)

Vollmer

Villagaray

Castro

et al. 2018

Plant Cell Tiss Organ Cult

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“…С использованием метода дроплет-витрификации созданы криоколлекции многочисленных образцов следующих культур (табл. 1): банана в Бельгии (Panis et al, , 2009; лука и чеснока в Южной Корее (Kim et al, 2006(Kim et al, , 2009 и Германии (Keller et al, 2016); картофеля в Перу (Panta et al, 2015;Vollmer et al, 2016Vollmer et al, , 2017, США (Bamberg et al, 2016;Jenderek, Reed, 2017) и России (Shvachko, Gavrilenko, 2011;Ukhatova et al, 2017b;Gavrilenko et al, 2018); малины и ежевики в США (Reed, DeNoma, 2012;Jenderek, Reed, 2017 Метод Cryo-plate, сочетающий приемы инкапсуляции-дегидратации и дроплет-витрификации, является самым новым (Yamamoto et al, 2011(Yamamoto et al, , 2012(Yamamoto et al, , 2015Valle Arizaga et al, 2017). В этом методе апексы микропобегов in vitro растений прикрепляют тонким слоем альгината кальция к алюминиевым крио-пластинам, обрабатывают их растворами LS и PVS, а затем быстро погружают криопластины в жидкий азот (Yamamoto et al, 2011).…”

Section: Cryoconservation Methods For Vegetatively Propagated Cropsunclassified

“…О необходимости проведения криоконсервации дополнительных эксплантов судят по результатам первой повторности: если частота регенерации превышает 30%, то вторую повторность не делают. Если частота регенерации составляет 20-30% -выполняют вторую повторность, если ниже 20%, то для таких образцов подбирают более специфичный протокол (Vollmer et al, 2016(Vollmer et al, , 2017.…”

Section: регламенты и стандарты для формирования криоколлекцийunclassified

An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

Супрун¹,

Насонов²,

Lоbodina³

et al. 2018

PBT&B

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“…В настоящее время наиболее часто применяется метод дроплетвитрификации, разработанный Panis et al (2005) для криоконсервации образцов банана. Данный метод был многократно модифицирован в разных лабора ториях и используется в крупнейших мировых генбанках для создания криоколлекций картофеля (Kim et al, 2006;Panta et al, 2015;Vollmer et al, 2016Vollmer et al, , 2017Ухатова и др., 2017;Jenderek, Reed, 2017;Гавриленко и др., 2019). Подробное сравнение различных модификаций метода дроплетвитрификации проведено в нашей другой работе (Гавриленко и др., 2019).…”

Section: Introductionunclassified

Investigation of the post-cryogenic regeneration ability of potato varieties under different cultivation conditions

et al. 2019

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Cryopreservation provides long-term storage of the gene pool of potato varieties in cryobanks at extremely low temperatures. Currently, droplet vitrification is the most widely used method for cryopreservation of potato varieties, which is constantly improving to increase the regeneration rates of the stored plant material. Different modifications of this method are used in the world’s leading potato genebanks. This paper presents the results of studying the effect of cultivation conditions after plunging into liquid nitrogen and thawing of shoots tips and axillary buds of in vitro plants on their postcryogenic recovery. The droplet-vitrification method modified at VIR was used for cryopreservation. The factor “prolonged dark incubation of explants” did not have a significant effect on the frequency of post-cryogenic regeneration of the studied varieties except for one variety (Krepysh), for which a significant increase in the regeneration rate was observed for the shoot tips cultivated in the darkness compared to the cultivation under the photoperiod 16/8 hours (light/darkness). The frequency of post-cryogenic regeneration of shoot tips was higher than that of the axillary buds for all varieties; however, these differences were significant (p < 0.05) only in two cases: for the variety Udacha (a photoperiod of 16/8 hours) and for the variety Krepysh (the dark incubation). The results of two-factor analysis of variance indicate that there is no effect of interaction of factor 1 (prolonged dark incubation) and factor 2 (explant type) on the ability of varieties to post-cryogenic recovery. Taking into account the obtained results, the further cryopreservation of an extended subset of 9 varieties was carried out using shoot tips, which, after freezing-thawing, were cultivated under the photoperiod of 16/8 hours. The frequency of post-cryogenic regeneration of these varieties varied from 30 to 60 %. A significant effect of genotype on postcryogenic recovery has been established. The ability of varieties to regenerate shoots after freezing and thawing was not related to the values of morphogenic indices of in vitro plants. The age of the meriklons (2–4 years) did not significantly affect either the morphogenic indices or the frequency of post-cryogenic regeneration.

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A large-scale viability assessment of the potato cryobank at the International Potato Center (CIP)

Cited by 29 publications

References 12 publications

Cryopreserved potato shoot tips showed genotype-specific response to sucrose concentration in rewarming solution (RS)

Cryopreserved potato shoot tips showed genotype-specific response to sucrose concentration in rewarming solution (RS)

An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

Investigation of the post-cryogenic regeneration ability of potato varieties under different cultivation conditions

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