A large volume particulate and water multi-sampler with in situ preservation for microbial and biogeochemical studies

Abstract

A new tool was developed for large volume sampling to facilitate marine microbiology and biogeochemical studies. It was developed for remotely operated vehicle and hydrocast deployments, and allows for rapid collection of multiple sample types from the water column and dynamic, variable environments such as rising hydrothermal plumes. It was used successfully during a cruise to the hydrothermal vent systems of the Mid-Cayman Rise. The Suspended Particulate Rosette V2 large volume multi-sampling system allows for the collection of 14 sample sets per deployment. Each sample set can include filtered material, whole (unfiltered) water, and filtrate. Suspended particulate can be collected on filters up to 142 mm in diameter and pore sizes down to 0.2 μm. Filtration is typically at flowrates of 2 L min-1. For particulate material, filtered volume is constrained only by sampling time and filter capacity, with all sample volumes recorded by digital flowmeter. The suspended particulate filter holders can be filled with preservative and sealed immediately after sample collection. Up to 2 L of whole water, filtrate, or a combination of the two, can be collected as part of each sample set. The system is constructed of plastics with titanium fasteners and nickel alloy spring loaded seals. There are no ferrous alloys in the sampling system. Individual sample lines are prefilled with filtered, deionized water prior to deployment and remain sealed unless a sample is actively being collected. This system is intended to facilitate studies concerning the relationship between marine microbiology and ocean biogeochemistry.

Observations of bubbles in natural seep flares at MC 118 and GC 600 using in situ quantitative imaging

“…Two samplers were used on each dive; both samplers were retrofitted with funnel‐shaped bubble collectors. Detailed water samples at selected points in and around the bubble plumes were collected with the SUPR (Suspended Particulate Rosette) sampling system [ Breier et al ., ], which enables fine‐scale sampling from remotely operated vehicles. During this cruise, fourteen 2 L bottles were served by the SUPR sampler.…”

Predicting the response of the deep-ocean microbiome to geochemical perturbations by hydrothermal vents

“…From there, the ROV then rose directly to the non-buoyant plume height of 200 m. Synoptic particulate samples were taken and the Al/(Al þ Fe þ Mn) ratio of these particles determined to confirm that sampling occurred within the plume, as reported by Sheik et al (in press). The field methodology for this ROV-based plume sampling was described in Breier et al (2009Breier et al ( , 2014. The latter study includes a Supplementary video showing this approach.…”

New applications of KNiFC-PAN resin for broad scale monitoring of radiocesium following the Fukushima Dai-ichi nuclear disaster

“…Most recently, an instrument capable of capturing cesium samples in situ, a Rad verison of a Clio SUPR Sampler was developed by John Breier and others at the Woods Hole Oceanographic Institute [1]. The Rad Clio SUPR sampler was deployed on an autonomous vehicle called a JetYak, (a vehicle resembling a kayak that can be propelled remotely).…”