A laser-activated multifunctional targeted nanoagent for imaging and gene therapy in a mouse xenograft model with retinoblastoma Y79 cells

Wu, Mingxing; Xiong, Haibo; Zou, Hongmi; Li, Meng; Li, Pan; Zhou, Yu; Xu, Yan; Jia, Jian; Liu, Fengqiu; Zhao, Hongyun; Wang, Zhigang; Zhou, Xiyuan

doi:10.1016/j.actbio.2018.02.006

Cited by 21 publications

(17 citation statements)

References 61 publications

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“…In tumor tissues, due to the lack of vascular supporting tissue, leaky blood vessels and capillary pores are easily formed. Nanoparticles of appropriate size (100 nm to 2 µm) can passively accumulate in tumor tissues, which is called the EPR effect (Wu et al., 2018 ). CMT NPs have good EPR effect conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Nanomedicine is a type of submicron drug delivery system designed to improve drug delivery to tumors while reducing systemic side effects, which has been widely studied in the oncology field in recent years (de Maar et al., 2020 ). There are several principles for targeting oncology drugs: passive targeting (relying mainly on enhanced permeability and retention (EPR) effects) (Nichols & Bae, 2014 ; Wang et al., 2018 ), active targeting (using vectors modified with tumor-specific targeting antibodies, magnetic targeting) (Wu et al., 2018 ; Guo et al., 2019 ), or triggered release (nanocarrier release drugs under thermal, ultrasonic, or light effects) (Arranja et al., 2017 ; Wang et al., 2020 ). Previously, folic acid-targeted liposome nanoparticles and magnetic targeted gold nanocages have been applied to the imaging and treatment of Rb with initial success (Wu et al., 2018 ; Wang et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…There are several principles for targeting oncology drugs: passive targeting (relying mainly on enhanced permeability and retention (EPR) effects) (Nichols & Bae, 2014 ; Wang et al., 2018 ), active targeting (using vectors modified with tumor-specific targeting antibodies, magnetic targeting) (Wu et al., 2018 ; Guo et al., 2019 ), or triggered release (nanocarrier release drugs under thermal, ultrasonic, or light effects) (Arranja et al., 2017 ; Wang et al., 2020 ). Previously, folic acid-targeted liposome nanoparticles and magnetic targeted gold nanocages have been applied to the imaging and treatment of Rb with initial success (Wu et al., 2018 ; Wang et al., 2020 ). However, considering the poor stability of liposome nanoparticles and the high cost of gold nanocages, the exploration of new nanomaterials has become a research direction.…”

Section: Introductionmentioning

confidence: 99%

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Multimodal imaging and photothermal synergistic immunotherapy of retinoblastoma with tuftsin-loaded carbonized MOF nanoparticles

Zou

et al. 2022

Drug Delivery

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Retinoblastoma (Rb) represents 3% of all childhood malignancies and seriously endangers children's lives and quality of life. Early diagnosis and treatment can save children's vision as much as possible. Multifunctional nanoparticles have become a research hotspot in recent years and are expected to realize the integration of early diagnosis and early treatment. Therefore, we report a nanoparticle with dual-mode imaging, photothermal therapy, and immune activation: carbonized MOF nanoparticles (CM NPs) loaded with the immune polypeptide tuftsin (CMT NPs). The dual-mode imaging ability, antitumor effect, and macrophage immunity activation ability of these nanoparticles combined with laser irradiation were studied. The biosafety of CMT NPs was detected. The multifunctional magnetic nanoparticles enhanced photoacoustic (PA) and magnetic resonance (MR) imaging in vivo and in vitro , facilitating diagnosis and efficacy evaluation. The combined effect of CMT NPs and laser irradiation was recorded and verified. Through the accumulation of magnetic field nanoparticles in tumors, the photothermal conversion of nanoparticles under laser irradiation led directly to tumor apoptosis/necrosis, and the release of tuftsin induced macrophage M1-type activation, resulting in antitumor immune effects. Enhanced PA/MR imaging CMT NPs have great potential in dual-mode image-guided laser/immune cotherapy. The nanoparticles have high biosafety and have potential in cancer treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multimodal imaging and photothermal synergistic immunotherapy of retinoblastoma with tuftsin-loaded carbonized MOF nanoparticles

Zou

et al. 2022

Drug Delivery

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“…Permeability was also increased, with wall pores approximately 380–780 nm in diameter. Therefore, TMUCA diameters were adjusted to approximately 500 nm for easy passage through vascular endothelial cells and improved molecular imaging (Liu and Huang, 2011 ; Song et al, 2015 ; Wu et al, 2018 ; Zheng et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Synergic fabrication of pembrolizumab loaded doxorubicin incorporating microbubbles delivery for ultrasound contrast agents mediated anti-proliferation and apoptosis

Liu

Chen

et al. 2021

Drug Delivery

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This study evaluated pembrolizumab-conjugated, doxorubicin (DOX)-loaded microbubbles (PDMs) in combination with ultrasound (US) as molecular imaging agents for early diagnosis of B cell lymphomas, and as a targeted drug delivery system. Pembrolizumab, a monoclonal CD20 antibody, was attached to the surfaces of DOX-loaded microbubbles. PDM binding to B cell lymphoma cells was assessed using immunofluorescence. The cytotoxic effects of PDMs in combination with ultrasound (PDMs þ US) were evaluated in vitro in CD20þ and CD20-cell lines, and its antitumor activities were assessed in Raji (CD20þ) and Jurkat (CD20-) lymphoma cell-grafted mice. PDMs specifically bound to CD20þ cells in vitro and in vivo. Contrast enhancement was monitored in vivo via US. PDM peak intensities and contrast enhancement durations were higher in Raji than in Jurkat cell-grafted mice (p < 0.05). PDMs þ US treatment resulted in improved antitumor effects and reduced systemic toxicity in Raji cell-grafted mice compared with other treatments (p < .05). Our results showed that PDMs þ US enhanced tumor targeting, reduced systemic toxicity, and inhibited CD20þ B cell lymphoma growth in vivo. Targeted PDMs could be employed as US molecular imaging agents for early diagnosis, and are an effective targeted drug delivery system in combination with US for CD20þ B cell malignancy treatment.

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“…When the absorbed optical energy is above a specific threshold, it leads to a local temperature increase required for the vaporization. ODV-based applications also include imaging and therapy [21,22]. Moreover, the applications can be extended to dual mode photoacoustic and ultrasound imaging and image-guided cancer therapy [19,23,24,25].…”

Section: Introductionmentioning

confidence: 99%

Synchronized Optical and Acoustic Droplet Vaporization for Effective Sonoporation

Liu

Huang

2019

Pharmaceutics

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Inertial cavitation-based sonoporation has been utilized to enhance treatment delivery efficacy. In our previous study, we demonstrated that tumor therapeutic efficacy can be enhanced through vaporization-assisted sonoporation with gold nanodroplets (AuNDs). Specifically, the AuNDs were vaporized both acoustically (i.e., acoustic droplet vaporization, ADV) and optically (i.e., optical droplet vaporization, ODV). A continuous wave (CW) laser was used for ODV in combination with an ultrasound pulse for ADV. Although effective for vaporization, the use of a CW laser is not energy efficient and may create unwanted heating and concomitant tissue damage. In this study, we propose the use of a pulsed wave (PW) laser to replace the CW laser. In addition, the PW laser was applied at the rarefaction phase of the ultrasound pulse so that the synergistic effects of ADV and ODV can be expected. Therefore, a significantly lower laser average power can be expected to achieve the vaporization threshold. Compared to the CW laser power at 2 W/cm2 from the previous approach, the PW laser power was reduced to only 0.2404 W/cm2. Furthermore, we also demonstrate in vitro that the sonoporation rate was increased when the PW laser was applied at the rarefaction phase. Specifically, the vaporization signal, the inertial cavitation signal, and the sonoporation rate all displayed a 1-µs period, which corresponded to the period of the 1-MHz acoustic wave used for ADV, as a function of the relative laser delay. The increased sonoporation rate indicates that this technique has the potential to enhance sonoporation-directed drug delivery and tumor therapy with a lower laser power while keeping the cell death rate at the minimum. Photoacoustic imaging can also be performed at the same time since a PW laser is used for the ODV.

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A laser-activated multifunctional targeted nanoagent for imaging and gene therapy in a mouse xenograft model with retinoblastoma Y79 cells

Cited by 21 publications

References 61 publications

Multimodal imaging and photothermal synergistic immunotherapy of retinoblastoma with tuftsin-loaded carbonized MOF nanoparticles

Multimodal imaging and photothermal synergistic immunotherapy of retinoblastoma with tuftsin-loaded carbonized MOF nanoparticles

Synergic fabrication of pembrolizumab loaded doxorubicin incorporating microbubbles delivery for ultrasound contrast agents mediated anti-proliferation and apoptosis

Synchronized Optical and Acoustic Droplet Vaporization for Effective Sonoporation

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