A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

Feng, Liang; Hui, Chen; Suyeoka, Genn; Liu, Xiaorong

doi:10.3791/50440-v

Cited by 1 publication

(1 citation statement)

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“…Rodent models of glaucoma have been widely used (Aihara et al, 2003;Grozdanic et al, 2003;Mabuchi et al, 2003;Feng et al, 2013a;Liu et al, 2020) because they share basic retinal structures with the human retina and offer genetic tools for in vivo manipulations (John, 2005;Howell et al, 2008). However, rodents have small eyes with thin layers, which impose challenges for investigating structural changes in retinal diseases.…”

Section: Introductionmentioning

confidence: 99%

Comparative In Vivo Imaging of Retinal Structures in Tree Shrews, Humans, and Mice

Grannonico,

Miller,

Liu

et al. 2024

eNeuro

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Rodent models, such as mice and rats, are commonly used to examine retinal ganglion cell damage in eye diseases. However, as nocturnal animals, rodent retinal structures differ from primates, imposing significant limitations in studying retinal pathology. Tree shrews (Tupaia belangeri) are small, diurnal paraprimates that exhibit superior visual acuity and color vision compared with mice. Like humans, tree shrews have a dense retinal nerve fiber layer (RNFL) and a thick ganglion cell layer (GCL), making them a valuable model for investigating optic neuropathies. In this study, we applied high-resolution visible-light optical coherence tomography to characterize the tree shrew retinal structure in vivo and compare it with that of humans and mice. We quantitatively characterize the tree shrew's retinal layer structure in vivo, specifically examining the sublayer structures within the inner plexiform layer (IPL) for the first time. Next, we conducted a comparative analysis of retinal layer structures among tree shrews, mice, and humans. We then validated our in vivo findings in the tree shrew inner retina using ex vivo confocal microscopy. The in vivo and ex vivo analyses of the shrew retina build the foundation for future work to accurately track and quantify the retinal structural changes in the IPL, GCL, and RNFL during the development and progression of human optic diseases.

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