A laser Raman spectroscopic study of the interaction of calf-thymus DNA with Cu(II) and Pb(II) ions: metal ion binding and DNA conformational changes

Tajmir-Riahi, H.A.; Langlais, Marc; Savoie, Rodrigue

doi:10.1093/nar/16.2.751

Cited by 106 publications

(80 citation statements)

References 27 publications

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“…This peak is relatively insensitive to the conformation of the polynucleotide and many investigators have followed the suggestion of Tsuboi et al (9) that it be used as an internal standard in conformational studies. On the other hand, Raman studies of aqueous DNA solutions have shown that this band can be affected in intensity as well as in frequency when metal ions are present in the solution (10)(11)(12). Just how these changes are related to the extent and type of metal binding by the phosphate groups is not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Vibrational spectroscopic study of the interaction of metal ions with diethyl phosphate, a model for biological systems

Stangret¹,

Savoie²

1992

Can. J. Chem.

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JANUSZ STANGRET and RODRIGUE SAVOIE. Can. J. Chem. 70, 2875Chem. 70, (1992. We have studied, using Raman and infrared spectroscopy, the interaction of various monovalent, divalent, and trivalent metal cations with the PO, group of diethyl phosphate in aqueous solution, at meta1:phosphate ratios ranging from 1 :40 to 6 : 1. The results show that the metal cations interact with the phosphate groups in various ways and to different extents. Spectral characteristics for some specific types of interactions are presented, namely, for direct metal binding by one of the oxygen atoms in the PO; group, the formation of water-separated ion pairs, and non-specific electrostatic interactions.JANUSZ STANGRET et RODRIGUE SAVOIE. Can. J. Chem. 70, 2875 (1 992). Nous avons etudii par spectroscopie Raman et infrarouge l'interaction de divers cations mttalliques monovalents, divalents et trivalents avec le groupe charge PO, du phosphate de diethyle en solution aqueuse, a des rapports metal : phosphate variant de 1 : 40 5 6 : 1. Les resultats demontrent que les ions se distinguent tant par leur affinite pour le groupement phosphate que par leur f a~o n d'interagir avec celui-ci. On prtsente diverses caracttristiques spectrales permettant d'identifier certains types d'interaction, notamment la fixation directe par l'un des atomes d'oxygkne du groupement phosphate, la formation de paires d'ions stparees par molCcules d'eau et les interactions non specifiques de nature electrostatique.

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Section: Introductionmentioning

confidence: 99%

Vibrational spectroscopic study of the interaction of metal ions with diethyl phosphate, a model for biological systems

Stangret¹,

Savoie²

1992

Can. J. Chem.

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“…Duguid et al (1996) have observed similar results. The 784 cm −1 band is the composite of two major bands, one due to the cytosine ring breathing mode (Tajmir-Riahi et al, 1988) and the other due to phosphosiester symmetric stretching vibration (Dam et al, 2002). The cytosine vibration is sensitive to deputation exocyclic substitution while the phosphodiester vibration is less sensitive to deputation.…”

Section: Resultsmentioning

confidence: 99%

Structural Analysis of DNA Interactions with Magnesium Ion Studied by Raman Spectroscopy

M.¹

2011

American Journal of Biochemistry and Biotechnology

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Problem statement:In the present study, FT Raman spectroscopy had been used to extend our knowledge about Magnesium ion -DNA interactions at various volume ratios (1:50, 1:20, 1:10 and 1:5). Approach: The analysis of FT Raman data supported the existence of structural specificities in the interaction and also the stability of DNA secondary structure. Results: Results from the Raman spectra clearly indicate that the interaction of Magnesium ion with DNA is mainly through the phosphate groups of DNA with negligible change of the B-conformation of DNA at all the volume ratios studied. For example, band at 1079 cm −1 is assigned to the symmetrical stretching vibration of the nucleic acid phosphodioxy (PO-2) group. This band in the order 1079→ 1075→ 1070→ 1066→ 1063 cmat all Magnesium ion DNA concentrations studied. Similarly, Raman band at 845 cm −1 due to antisymmetrical phosphodiester (O-P-O) stretching of DNA. Conclusion: Magnesium ion interaction with the DNA phosphate is weak in comparison to interactions with the bases. On the other hand, the Raman signature of B-DNA is largely unperturbed by magnesium ion, suggesting much weaker interactions.

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“…The values of the global relaxation time suggest also the existence of a vibrational relaxation time, because the reorientational movement is much more slower for the DNA macromolecule in aqueous solution. Particularly, the absence of reorientational broadening [13,[15][16][17], the cytosine ring breathing mode at 787 cm −1 [7,16,[18][19][20], the DNA backbone PO 2 − symmetric stretching vibration at 1094 cm −1 [12,16,19], the purines (dA, dG) and pyrimidines (dT, dC) residues band at 1376 cm −1 [17][18][19], the guanine (N-7) and adenine rings vibration at 1489 cm −1 [16,17] and the purines (dG, dA) 1578 cm −1 vibration ( [21] and references therein) of calf-thymus DNA, at different Mn 2+ ions concentrations, in the presence of Na + ions, are summarized in Tables 1, 2 and 4, 5, respectively.…”

Section: CM Muntean and I Bratu / (Sub)picosecond Dynamics In Mndnmentioning

confidence: 99%

Molecular relaxation processes in calf-thymus DNA, in the presence of Mn²⁺and Na⁺ions: A Raman spectroscopic study

Muntean

Bratu

2008

Spectroscopy

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Abstract. In this paper the Raman total half bandwidths of calf-thymus DNA vibrations have been measured as a function of Mn 2+ ion concentration (0-600 mM), in the presence of two concentrations of Na + cations, respectively. The dependencies of the half bandwidths and of the global relaxation times on DNA molecular subgroup structure, on Mn 2+ and Na + ions concentrations, respectively, are reported. It is shown that changes in the (sub)picosecond dynamics of molecular subgroups in calf-thymus DNA can be monitored with Raman spectroscopy.In this study the Raman band parameters for the vibrations at 729 cm1376 cm −1 (dA, dG, dT, dC), 1489 cm −1 (dG, dA) and 1578 cm −1 (dG, dA) of calf thymus DNA are presented. The fullwidths at half-height (FWHH) of the bands in calf-thymus DNA are typically in the wavenumber range from 9 to 33.5 cm −1 . It can be observed that the molecular relaxation processes studied in this work, have a global relaxation time smaller than 1.179 ps and larger than 0.317 ps.Mn 2+ -induced DNA structural changes result for the vibrations at 729 cm −1 and 787 cm −1 in smaller global relaxation times, and larger half bandwidths, respectively, as compared to the starting value of 0 mM Mn 2+ . The vibrational energy transfer processes of these two subgroups (dA, dC), respectively, are slower in the case of DNA samples at 10 mM NaCl, as compared to the corresponding DNA samples at 150 mM NaCl. However, the behaviour of the global relaxation times characteristic to the bands at 729 and 787 cm −1 is similar with respect to manganese(II) ions concentration, in the case of the two values of Na + ions content, respectively.On the contrary, the molecular dynamics is slower for the base vibrations at 1376, 1489 and 1578 cm −1 , in the case of DNA samples at 150 mM NaCl, as compared to the corresponding samples at lower Na + concentration, in almost all Mn 2+ ions concentration range. The molecular relaxation processes in these three cases, respectively, are quite different for the corresponding samples with different Na + ions content, upon increasing divalent manganese ions concentration.The molecular dynamics characterizing the band near 1094 cm −1 of the DNA backbone PO 2 − symmetric stretching vibration is faster upon increasing the Mn 2+ ions concentration between 0-600 mM and seems not to be influenced by the Na + ions content, specific to our experimental conditions.

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A laser Raman spectroscopic study of the interaction of calf-thymus DNA with Cu(II) and Pb(II) ions: metal ion binding and DNA conformational changes

Cited by 106 publications

References 27 publications

Vibrational spectroscopic study of the interaction of metal ions with diethyl phosphate, a model for biological systems

Vibrational spectroscopic study of the interaction of metal ions with diethyl phosphate, a model for biological systems

Structural Analysis of DNA Interactions with Magnesium Ion Studied by Raman Spectroscopy

Molecular relaxation processes in calf-thymus DNA, in the presence of Mn²⁺and Na⁺ions: A Raman spectroscopic study

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A laser Raman spectroscopic study of the interaction of calf-thymus DNA with Cu(II) and Pb(II) ions: metal ion binding and DNA conformational changes

Cited by 106 publications

References 27 publications

Vibrational spectroscopic study of the interaction of metal ions with diethyl phosphate, a model for biological systems

Vibrational spectroscopic study of the interaction of metal ions with diethyl phosphate, a model for biological systems

Structural Analysis of DNA Interactions with Magnesium Ion Studied by Raman Spectroscopy

Molecular relaxation processes in calf-thymus DNA, in the presence of Mn2+and Na+ions: A Raman spectroscopic study

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Molecular relaxation processes in calf-thymus DNA, in the presence of Mn²⁺and Na⁺ions: A Raman spectroscopic study